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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1649-1656.doi: 10.3724/SP.J.1006.2012.01649

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Expression Analysis of GhSAMDC at Low Temperature Stress in Cotton (Gossypium hirsutum L.)

GENG Wei-Dong,LI Yan-Jun,ZHANG Xin-Yu,ZHU Hua-Guo*,SUN Jie*   

  1. College of Agriculture / The key Laboratory of Oasis Eco-agriculture Xinjiang Production and Construction Group, Shihezi University, Shihezi 832003, China
  • Received:2012-01-08 Revised:2012-04-20 Online:2012-09-12 Published:2012-06-04
  • Contact: 朱华国, Email: zhgroger@sohu.com; 孙杰, E-mail: sunjiezh@yahoo.com.cn

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Abstract: S-adenosylmethionine decarboxylase is one of the key enzymes in polyamine biosynthesis. With the increase of polyamine accumulation in plants, SAMDC can improve crop tolerance to environment stresses. We cloned the S-adenosylmethionine decarboxylase gene from Gossypium hirsutum by RACE and RT-PCR, named GhSAMDC (GenBank accession number: JN020148). The cDNA full length was 1 874 bp, with three open reading frames, i.e. tiny ORF (tORF), upstream ORF (uORF) and main ORF (mORF). The mORF was 1 064 bp encoding 355 amino acids with a molecular mass of 38.25 kD, and its deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain). The complete genomic DNA sequence of GhSAMDC was 2 743 bp in length with three introns, all of which were located in 5' UTR, and one lay in theuORF. Phylogenetic analysis showed GhSAMDC was the closest to that in Vitis vinifera, and Real-time PCR suggested that GhSAMDC was induced by cold stress. It showed lower gene expression level in cold resistant cultivar Xinluzao 33 than in cold sensitive cultivar Xinluzao 1.

Key words: Cotton (Gossypium hirsutum L.), S-adenosylmethionine decarboxylase, Sequence analysis, qPCR, Expression at low temperature

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