Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1649-1656.doi: 10.3724/SP.J.1006.2012.01649

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Expression Analysis of GhSAMDC at Low Temperature Stress in Cotton (Gossypium hirsutum L.)

GENG Wei-Dong,LI Yan-Jun,ZHANG Xin-Yu,ZHU Hua-Guo*,SUN Jie*   

  1. College of Agriculture / The key Laboratory of Oasis Eco-agriculture Xinjiang Production and Construction Group, Shihezi University, Shihezi 832003, China
  • Received:2012-01-08 Revised:2012-04-20 Online:2012-09-12 Published:2012-06-04
  • Contact: 朱华国, Email: zhgroger@sohu.com; 孙杰, E-mail: sunjiezh@yahoo.com.cn

PDF

1299

Abstract: S-adenosylmethionine decarboxylase is one of the key enzymes in polyamine biosynthesis. With the increase of polyamine accumulation in plants, SAMDC can improve crop tolerance to environment stresses. We cloned the S-adenosylmethionine decarboxylase gene from Gossypium hirsutum by RACE and RT-PCR, named GhSAMDC (GenBank accession number: JN020148). The cDNA full length was 1 874 bp, with three open reading frames, i.e. tiny ORF (tORF), upstream ORF (uORF) and main ORF (mORF). The mORF was 1 064 bp encoding 355 amino acids with a molecular mass of 38.25 kD, and its deduced protein had two highly conserved function domains (proenzyme cleavage site and PEST domain). The complete genomic DNA sequence of GhSAMDC was 2 743 bp in length with three introns, all of which were located in 5' UTR, and one lay in theuORF. Phylogenetic analysis showed GhSAMDC was the closest to that in Vitis vinifera, and Real-time PCR suggested that GhSAMDC was induced by cold stress. It showed lower gene expression level in cold resistant cultivar Xinluzao 33 than in cold sensitive cultivar Xinluzao 1.

Key words: Cotton (Gossypium hirsutum L.), S-adenosylmethionine decarboxylase, Sequence analysis, qPCR, Expression at low temperature

[1]Alcázar R, Marco F, Cuevas J C, Patron M, Ferrando A, Carrasco P, Tiburcio A F, Altabella T. Involvement of polyamines in plant response to abiotic stress. Biotechnol Lett, 2006, 28: 1867–1876

[2]Kusano T, Berberich T, Tateda C, Takahashi Y. Polyamines: essential factors for growth and survival. Planta, 2008, 228: 367–381

[3]Shen W Y, Nada K, Tachibana S. Involvement of polyamines in the chilling tolerance of cucumber cultivars. Plant Physiol, 2000, 124: 431–439

[4]Cuevas J C, López-Cobollo R, Alcázar R, Zarza X, Koncz C, Altabella T, Salinas J, Tiburcio A F, Ferrando A. Putrescine is involved in Arabidopsis freezing tolerance and cold acclimation by regulating abscisic acid levels in response to low temperature. Plant Physiol, 2008, 148: 1094–1105

[5]Franceschftti M, Hanfrey C, Scaramagli S, Torrigiani P, Bagni N, Burtin D, Anthony J M. Characterization of monocot and dicot plant S-adenosyl-L-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames. Biochem J, 2001, 353: 403–409

[6]Bhavna W, Manchikatla V R. Effect of increased polyamine biosynthesis on stress responses in transgenic tobacco by introduction of human S-adenosylmethionine gene. Plant Sci, 2003, 164: 727–734

[7]Cheng L, Zou Y J, Ding S L, Zhang J J, Yu X L, Cao J S, Lu G. Polyamine accumulation in transgenic tomato enhances the tolerance to high temperature stress. J Integr Plant Biol, 2009, 51: 489–499

[8]Momtaz O A, Hussein E M, Fahmy E M, Ahmed S E. S-adenosylmethionine decarboxylase gene for polyamine accumulation in Egyptian cotton Giza 88 and Giza 90. GM Crops, 2010, 1: 257–266

[9]Zhao L L, Song L Q, You C X, Moriguchi T, Hao Y J. Functional characterization of the apple MdSAMDC2 gene by ectopic promoter analysis and over-expression in tobacco. Biol Plant, 2010, 54: 631–638

[10]Liu Z-Y(刘志勇), Wang X-X(王孝宣), Gao J-C(高建昌), Guo Y-M(国艳梅), Du Y-C(杜永臣), Ye X-L(叶雪凌). Cloning and sequence analysis of a S-adenosylmethionine decarboxylase gene (SlSAMDC1) in tomato. Acta Hort Sin (园艺学报), 2008, 35(8): 1137–1146 (in Chinese with English abstract)

[11]Liu J-X(刘金仙), Que Y-X(阙友雄), Guo J-L(郭晋隆), Xu L-P(许莉萍), Xu J-S(徐景升), Chen R-K(陈如凯). Molecular cloning of sugarcane S-adenosylmethionine decarboxylase gene (Sc-SAMDC) and its expression analysis. Sci Agric Sin (中国农业科学), 2010, 43(7): 1448–1457 (in Chinese with English abstract)

[12]Lu J-X(陆俊杏), Lu K(卢坤), Zhang K(张凯), Cao T(曹廷), Li J-N(李加纳), Liang Y(梁颖). Cloning and analysis of SAMDC3 genes and their promoters from Brassicanapus. Genom Appl Biol (基因组学与应用生物学), 2010, 29(2): 215–224 (in Chinese with English abstract)

[13]Zeng X-Q(曾兴权), Wang C-Y(王长有), Zhang H(张宏), Wei Z-X(韦泽秀), Liu X-L(刘新伦), Wang Y-J(王亚娟), Ji W-Q(吉万全). Cloning and characterization of an up-regulated S-adenosylmethionine decarboxylas (SAMDC) gene induced by stripe rust (Puccinia striiformisf. sp. tritici) in wheat. J Triticeae Crops (麦类作物学报), 2011, 31(5): 811–818 (in Chinese with English abstract)

[14]Jiang J-X(蒋建雄), Zhang T-Z(张天真). Extraction of total rna in cotton tissues with CTAB-acidic phenolic method. Cotton Sci (棉花学报), 2003, 15(3): 166–167 (in Chinese with English abstract)

[15]Zhao W-J(赵文静), Xu J(徐洁), Bao Q-H(包秋华), Chen Y-F(陈永福), Zhang H-P(张和平). Selection of reference genes for Real-time quantitative PCR. Microbiol China (微生物学通报), 2010, 37(12): 1825−1829 (in Chinese with English abstract)

[16]Livaka K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 2001, 25: 402–408

[17]Stanley B A, PeggS A E, Holm I. Site of pyruvate formation and processing of mammalian S-adenosy-lmethionine decarboxylase preenzyme. J Biol Chem, 1989, 264: 21073–21079

[18]Rogers S, Wells R, Rechsteiner M. Amino acid sequence conunon to rapid degraded proteins: The PEST hypotheis. Science, 1986, 234: 364–368

[19]Zhang J-J(张佳景), Ding S-L(丁淑丽), Zhou Y-J(邹宜静), Cheng L(程琳), Lu G(卢钢). Research advance on S-adenosylmethionine decarboxylase in Plants. Chin J Cell Biol (细胞生物学杂志), 2008, 30(5): 622–628 (in Chinese with English abstract)

[20]Wang X-L(王小利), Liu X-X(刘晓霞), Wang S-Y(王舒颖), Yang Y-C(杨义成), Wu J-H(吴佳海). Cloning and differential expression analysis of S–adenosylmethionine decarboxylase gene FaSAMDC in tall fescue. Acta Pratac Sin (草业学报), 2011, 20(4): 169–179 (in Chinese with English abstract)
[1] HUANG Cheng, LIANG Xiao-Mei, DAI Cheng, WEN Jing, YI Bin, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, MA Chao-Zhi. Genome wide analysis of BnAPs gene family in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(3): 597-607.
[2] Meng-Ting YANG, Chun ZHANG, Zuo-Ping WANG, Hua-Wen ZOU, Zhong-Yi WU. Cloning and functional analysis of ZmbHLH161 gene in maize [J]. Acta Agronomica Sinica, 2020, 46(12): 2008-2016.
[3] GAO Shi-Wu,FU Zhi-Wei,CHEN Yun,LIN Zhao-Li,XU Li-Ping,GUO Jin-Long. Cloning and expression analysis of metallothionein family genes in response to heavy metal stress in sugarcane (Saccharum officinarum L.) [J]. Acta Agronomica Sinica, 2020, 46(02): 166-178.
[4] TIAN Wen-Gang,ZHU Xue-Feng,SONG Wen,CHENG Wen-Han,XUE Fei,ZHU Hua-Guo. Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana [J]. Acta Agronomica Sinica, 2019, 45(7): 1017-1028.
[5] YIN Long-Fei,WANG Zhao-Yang,WU Zhong-Yi,ZHANG Zhong-Bao,YU Rong. Cloning and functional analysis of ZmGRAS31 gene in maize [J]. Acta Agronomica Sinica, 2019, 45(7): 1029-1037.
[6] HU Hai-Yan,LIU Di-Qiu,LI Yun-Jing,LI Yang,TU Li-Li*. Identification of Promoter GhFLA1 Preferentially Expressed during Cotton fiber Elongation [J]. Acta Agron Sin, 2017, 43(06): 849-854.
[7] XU Yu-Chao,HOU Xi-Lin,XU Wei-Wei,SHEN Lu-Lu,ZHANG Shi-Lin,LIU Shi-Tuo,HU Chun-Mei. Cloning and Expression Analysis of Anthocyanidin Synthase Gene BrcANS from Purple Non-heading Chinese Cabbage [J]. Acta Agron Sin, 2016, 42(06): 850-859.
[8] WANG Xiao-Hong, ZHU Pan-Pan, LIANG Yan-Mei, HAN Shu-Mei, ZHAO Ai-Chun, WANG Chuan-Hong, LU Cheng, YU Mao-De. Molecular Cloning and Functional Analysis of Polygalacturonase-Inhibiting Protein Gene MaPGIP1 from Mulberry (Morus atropurpurea Roxb.) [J]. Acta Agron Sin, 2015, 41(09): 1361-1371.
[9] XU Nai-Yin,LI Jian. Identification of Ideal Test Environments for Multiple Traits Selection in Cotton Regional Trials [J]. Acta Agron Sin, 2014, 40(11): 1936-1945.
[10] YANG Chang-Qin,LIU Rui-Xian,ZHANG Guo-Wei,YANG Fu-Qiang,ZHOU Zhi-Guo. Sugar Metabolism and its Relationship with Fiber Strength Affected by Waterlogging during Flowering and Bolling Stage in Cotton [J]. Acta Agron Sin, 2014, 40(09): 1612-1618.
[11] XU Nai-Yin,LI Jian. Ecological Regionalization of Cotton Fiber Quality Based on GGE Biplot in Yangtze River Valley [J]. Acta Agron Sin, 2014, 40(05): 891-898.
[12] LIU Jing-Ran,LIU Jia-Jie,MENG Ya-Li,WANG You-Hua,CHEN Bing-Lin,ZHANG Guo-Wei,ZHOU Zhi-Guo. Effect of 6-BA and ABA Applications on Yield, Quality and Photosynthate Contents in Subtending Leaf of Cotton with Different Planting Dates [J]. Acta Agron Sin, 2013, 39(06): 1078-1088.
[13] LUO Mao,PENG Hua,SONG Rui,GAO Jian,PAN Guang-Tang,ZHANG Zhi-Ming. Functional Analysis of miRNA Resistant to Banded Leaf-Sheath Blight in Maize [J]. Acta Agron Sin, 2013, 39(05): 837-844.
[14] LI Wei,SHANG Hai-Hong,WANG Shao-Gan,FAN Sen-Miao,LI Jun-Wen,LIU Ai-Ying,SHI Yu-Zhen,GONG Ju-Wu,GONG Wan-Kui,WANG Tao,BAI Zhi-Chuan,YUAN You-Lu. Cloning and Expression Analysis of Three Aquaporin Genes in Upland Cotton (Gossypium hirsutum L.) [J]. Acta Agron Sin, 2013, 39(02): 222-229.
[15] XU Nai-Yin,ZHANG Guo-Wei,LI Jian,ZHOU Zhi-Guo. Evaluation of Cotton Regional Trial Environments Based on HA-GGE Biplot in the Yangtze River Valley [J]. Acta Agron Sin, 2012, 38(12): 2229-2236.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd