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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 837-844.doi: 10.3724/SP.J.1006.2013.00837


Functional Analysis of miRNA Resistant to Banded Leaf-Sheath Blight in Maize

LUO Mao1,**,PENG Hua2,**,SONG Rui2,GAO Jian2,PAN Guang-Tang2,*,ZHANG Zhi-Ming2,*   

  1. 1 Research Center for Drug Discovery of Luzhou Medical College, Luzhou 646000, China; 2 Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang 611130, China
  • Received:2012-09-03 Revised:2012-12-11 Online:2013-05-12 Published:2013-02-19
  • Contact: 潘光堂, 张志明, E-mail: panlab605@gmail.com, Tel: 028-86290917


microRNAs (miRNAs) are an extensive class of endogenous, non-coding, short (19–24 nt) RNA molecules, which directly involve in regulating gene expression at the post-transcriptional level and play important roles in the regulation of plant growth and development, and in response to various abiotic and biotic stress. However, the miRNAs-regulated networks and function in response to BLSB are not very clear in Zea mays. In our previous studies, miRNAs and their target genes in maize were excavated by bioinformatic prediction, and a total of 23 conserved miRNAs and 89 miRNA targets were predicted and identified by a web-based integrated computing system and WMD 3. In this paper, based on previous studies, we profiled the responsive functions of miRNAs under BLSB stress, preliminarily identified the precursor of miRNAs in BLSB-stressed Zea mays (R15 and Ye478) by using semi-quantitative RT-PCR and detected the level of mature miRNAs under BLSB stress, showing that some miRNAs play very important roles in response to BLSB in Zea mays.

Key words: miRNA, Semi-quantitative RT-PCR, QPCR, Zea mays

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