Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 845-854.doi: 10.3724/SP.J.1006.2013.00767

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Differentially Expressed Protein Profiling during Ear Floral Development between Maize Hybrid and Its Parents

GUO Bao-Jian,SONG Fang-Wei,FENG Wan-Jun,SUI Zhi-Peng,SUN Qi-Xin,NI Zhong-Fu*   

  1. State Key Laboratory for Agrobiotechnology / Key Laboratory of Crop Heterosis and Utilization, Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University / National Plant Gene Research Centre (Beijing), Beijing 100193, China
  • Received:2012-09-05 Revised:2012-12-16 Online:2013-05-12 Published:2013-02-22
  • Contact: 倪中福, E-mail: nzhf2002@yahoo.com.cn

Abstract:

To gain an insight into the molecular basis of heterosis related to kernel number per ear in maize, we established a differentially expressed protein profiling between highly heterotic hybrid Yuyu 22 and its parental lines Zong 3, 87-1 during ear floral development by using a combined methods of 2-DE and MALDI TOF MS. A total of 1290 protein spots were detected, among which 114 were found to be differentially expressed with a significant difference at P<0.05. The number of protein spots belongs to the models of expression in hybrid and uniparent but not in another parent (UPF1), hybrid is equal to the highly expressed parent (HDH), hybrid is equal to the mid-parent (MPE), hybrid is equal to the lowly expressed parent (LDH), up-regulated in hybrid (URH), down-regulated in hybrid (DRH), dominant expression of uniparental proteins but not in hybrids (UPnF1), hybrid-specific expressed protein spots (F1nBP) was 27, 25, 15, 13, 11, 11, 10, and 2, respectively. In addition, 104 proteins were identified by using MALDI TOF MS, which are involved in 12 functional categories, including metabolism, signal transduction, energy, gene transcription, protein synthesis, protein transport and storage, cell growth, cell division, cell structure, disease and defense, secondary metabolism, transposons, unknown and putative proteins. Taken together, expression differences between hybrid and its parents at protein abundances and multiple functions of in hybrid may contribute to the heterosis related to kernel number per ear.

Key words: Maize, Ear, Heterosis, Differentially expressed protein profiling

[1]Shull G H. Duplicate genes for capsule form in Bursa bursa-pastoris. Mol Gen Genet, 1914, 12: 97–149



[2]Romagnoli S, Maddaloni M, Livini C, Motto M. Relationship between gene expression and hybrid vigor in primary root tips of young maize (Zea mays L.) plantlets. Theor Appl Genet, 1990, 80: 769–775



[3]Sun Q X, Wu L M, Ni Z F, Meng F R, Wang Z K, Lin Z. Differential gene expression patterns in leaves between hybrids and their parental inbreds are correlated with heterosis in a wheat diallel cross. Plant Sci, 2004, 166: 651–657



[4]Zhang Y-R(张义荣), Yao Y-Y(姚颖垠), Peng H-R(彭惠茹), Zhang Q-B(张庆波), Ni Z-F(倪中福), Sun Q-X(孙其信). Progress in molecular genetic mechanism of plant heterosis. Prog Nat Sci (自然科学进展), 2009, 19(7): 697–703 (in Chinese with English abstract)



[5]Hoecker N, Keller B, Muthreich N, Chollet D, Descombes P, Piepho H P, Hochholdinger F. Comparison of maize (Zea mays L.) F1-hybrid and parental inbred line primary root transcriptomes suggests organ-specific patterns of nonadditive gene expression and conserved expression trends. Genetics, 2008, 179:1275–1283



[6]Fu Z Y, Jin X N, Ding D, Li Y L, Fu Z J, Tang J H. Proteomic analysis of heterosis during maize seed germination. Proteomics, 2011, 11: 1462–1472



[7]Bortiri E, Chuck G, Vollbrecht E, Rocheford T, Martienssen R, Hake S. Ramosa2 encodes a lateral organ boundary domain protein that determines the fate of stem cells in branch meristems of maize. Plant Cell, 2006, 18: 574–585



[8]Hoecker N, Lamkemeyer T, Sarholz B, Paschold A, Fladerer C, Madlung J, Wurster K, Stahl M, Piepho H P, Nordheim A, Hochholdinger F. Analysis of nonadditive protein accumulation in young primary roots of a maize (Zea mays L.) F(1)-hybrid compared to its parental inbred lines. Proteomics, 2008, 8: 3882–3894.



[9]Tang J H, Yan J B, Ma X Q, Teng W T, Wu W R, Dai J R, Dhillon B S, Melchinger A E, Li J S. Dissection of the genetic basis of heterosis in an elite maize hybrid by QTL mapping in an immortalized F2 population. Theor Appl Genet, 2010, 120: 333–340



[10]Ma X Q, Tang J H, Teng W T, Yan J B, Meng Y J, Li J S. Epistatic interaction is an important genetic basis of grain yield and its components in maize. Mol Breed, 2007, 20: 41–51



[11]Stupar R M, Springer N M. Cis-transcriptional variation in maize inbred Lines B73 and Mo17 leads to additive expression patterns in the F1 hybrid. Genetics, 2006, 173: 2199–2210



[12]Li B, Zhang D F, Jia G Q, Dai J R, Wang S C. Genome-wide comparisons of gene expression for yield heterosis in maize. Plant Mol Biol Rep, 2009, 27: 162–176



[13]Pea G, Ferron S, Gianfranceschi L, Krajewski P, Pè E. Gene expression nonadditivity in immature ears of a heterotic F1 maize hybrid. Plant Sci, 2008, 174: 17–24



[14]Bradford M M. A rapid and sensitive method for the quantization of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem, 1976, 72: 248–254



[15]Song X, Ni Z F, Yao Y Y, Xie C J, Li Z X, Wu H Y, Zhang Y H, Sun Q X. Wheat (Triticum aestivum L.) root proteome and differentially expressed root proteins between hybrid and parents. Proteomics, 2007, 7: 3538–3557



[16]Stuber C W, Lincoln S E, Wolff D W, Helentjaris T, Lander E S. Identi?cation of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics, 1992, 132: 823–839



[17]Lu H, Romero-Severson J, Bernarbo R. Genetic basis of heterosis explored by simple sequence repeat markers in a random-mated maize population. Theor Appl Genet, 2003, 107: 494–502



[18]Frascaroli E, Canè MA, Landi P, Pea G, Gianfranceschi L, Villa M, Morgante M, Pè M E. Classical genetic and quantitative trait loci analyses of heterosis in a maize hybrid between two elite inbred lines. Genetics, 2007, 176: 625–644



[19]Satoh-Nagasawa N, Nagasawa N, Malcomber S, Sakai H, Jackson D. A trehalose metabolic enzyme controls inflorescence architecture in maize. Nature, 2006, 441: 227–230



[20]Bao W-K(鲍文奎). Chance and challenge-40 years breeding research thinking. Plant J (植物杂志), 1990, (4): 4–5 (in Chinese)



[21]Manning K, Tor M, Poole M, Hong Y, Thompson A J, King G J, Giovannoni J J, Seymour G B. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nat Genet, 2006, 38: 948–952



[22]Shindo C, Lister C, Crevillen P, Nordborg M, Dean C. Variation in the epigenetic silencing of FLC contributes to natural variation in Arabidopsis vernalization response. Genes Dev, 2006, 20: 3079–3083



[23]Ni Z F, Kim E D, Ha M S, Lackey E, Liu J X, Zhang Y R, Sun Q X, Chen Z J. Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids. Nature, 2009, 457: 327–331



[24]He G, Zhu X, Elling A A, Chen L, Wang X, Guo L, Liang M, He H, Zhang H, Chen F, Qi Y, Chen R, Deng X W. Global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids. Plant Cell, 2010, 22: 17–33



[25]Groszmann M, Greaves I K, Albertyn Z I, Scofield G N, Peacock W J, Dennis E S. Changes in 24-nt siRNA levels in Arabidopsis hybrids suggest an epigenetic contribution to hybrid vigor. Proc Natl Acad Sci USA, 2011, 108: 2617–2622



[26]Shen H S, He H, Li J G, Chen W, Wang X C, Guo L, Peng Z Y, He G M, Zhong S W, Qi Y J, Terzaghi W, Deng X W. Genome-wide analysis of DNA methylation and gene expression changes in two Arabidopsis ecotypes and their reciprocal hybrids. Plant Cell, 2012, 24: 875–892



[27]Kumar A, Bennetzen J L. Plant retrotransposons. Annu Rev Genet, 1999, 33: 479–532



[28]Wu L M, Ni Z F, Meng F R, Lin Z, Sun Q X. Cloning and characterization of leaf cDNAs that are differentially expressed between wheat hybrids and their parents. Mol Genet Genomics, 2003, 270: 281–286



[29]Hoecker N, Keller B, Muthreich N, Chollet D, Descombes P, Piepho H P, Hochholdinger F. Comparison of maize (Zea mays L.) F1-hybrid and parental inbred line primary root transcriptomes suggests organ-speci?c patterns of nonadditive gene expression and conserved expression trends. Genetics, 2008, 179:1275–1283

[1] Liu En-Bo, Chen Jing, Li Hong-Xing, Yu Ning-Ning, Ren Bai-Zhao, Zhao Bin, Liu Peng, Zhang Ji-Wang. Shading inhibits ear and tassel development of summer maize by altering source-sink balance and regulating carbohydrate metabolism [J]. Acta Agronomica Sinica, 2026, 52(6): 1891-1901.
[2] Liang Jin-Yu, Yin Jia-De, Wang Hong-Li, Zhang Guo-Ping, Hou Hui-Zhi, Dong Bo, Ma Ming-Sheng. Estimation of leaf nitrogen content in dryland forage maize using UAV-based hyperspectral imaging and machine learning [J]. Acta Agronomica Sinica, 2026, 52(6): 1788-1801.
[3] Sun Shu-Feng, Xu Zhen-Nan, Huang Jia-Xin, Weng Jian-Feng, Li Xin-Hai. Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection [J]. Acta Agronomica Sinica, 2026, 52(5): 1291-1308.
[4] Zhang Ning-Ning, Teng Yu-Fei, Ren Na-Na, Wei Xing-Zhuo, Yan Shu-Hao, Fan Ke-Xin, Wang Yong-Hong, Chen Wen-Kang, Zhang Xing-Hua, Zhu Wan-Chao, Xu Shu-Tu, Xue Ji-Quan. Phenotypic evaluation and plasticity analysis of drought resistance in 201 maize inbred lines [J]. Acta Agronomica Sinica, 2026, 52(5): 1309-1325.
[5] Yang Xin-Yu, Cui Wen-Tao, Dilinigeer Alimu, Wang Kai-Xiang, Wu Peng-Hao, Ren Jiao-Jiao. Genome-wide association and genomic selection analysis of the number of leaves above the ear in maize [J]. Acta Agronomica Sinica, 2026, 52(5): 1573-1590.
[6] Han Ya-Xin, He Guan-Hua, Zhang Xiao-Qiong, Zhang Deng-Feng, Li Yong-Xiang, Liu Xu-Yang, Wang Tian-Yu, Li Yu, Zou Hua-Wen, Li Chun-Hui. Identification of maize lateral root density genes resources through integrated RNA-seq and BSA-seq analyses [J]. Acta Agronomica Sinica, 2026, 52(5): 1341-1352.
[7] Liu Xin-Meng, Ren Hao, Zhang Ji-Bo, Zhang Ji-Wang, Zhao Bin, Ren Bai-Zhao, Liu Peng, Wang Hong-Zhang. Physiological mechanisms of methyl jasmonate (MeJA) alleviating the effects of heat stress on ear differentiation in maize [J]. Acta Agronomica Sinica, 2026, 52(5): 1561-1572.
[8] Zhang Hong-Rong, Wang Fei-Er, Li Pan, Qiu Hai-Long, Zhu Jing, Zhao Lian-Hao, Nan Yun-You, He Wei, Fan Zhi-Long, Hu Fa-Long, Chai Qiang, Yin Wen. Photosynthetic characteristics of 20% reduced irrigation combined with 25% organic substitution for chemical fertilizer in increasing silage maize yield [J]. Acta Agronomica Sinica, 2026, 52(5): 1487-1500.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[11] Cai Hong-Wei, Yu Ai-Zhong, Jiang Ke-Qiang, Wang Peng-Fei, Wang Yu-Long, Huo Jian-Zhe, Pang Xiao-Neng, Yin Bo, Shang Yong-Pan. Key mechanisms underlying the enhancement of sweet maize yield through partial substitution of chemical fertilizers with organic manure in arid irrigation districts [J]. Acta Agronomica Sinica, 2026, 52(4): 1166-1180.
[12] Tian Hong-Li, Yang Yang, Fan Ya-Ming, Yi Hong-Mei, Guo Dan-Dan, Wang Feng-Ge, Zhao Jiu-Ran. A novel set of tri-allelic variant SNP loci suitable for maize variety identification [J]. Acta Agronomica Sinica, 2026, 52(4): 993-1005.
[13] Yang Rui, Chen Jing-Dong, Huang Ying, Zhang Xue-Kun, Zhou Deng-Wen, Liu Qing-Yun, Xu Jin-Song, Xie Ling-Li, Xu Ben-Bo. Study on breeding and cultivation strategies for winter rapeseed to cope with climate change in the lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2026, 52(4): 1153-1165.
[14] Jiang Jia-Hui, Jiang Bing-Zhi, Liu Guan-Ming, Wang Zhang-Ying, Tang Chao-Chen. Establishment and optimization of near-infrared spectroscopy models for quality traits of purple-fleshed sweet potato [J]. Acta Agronomica Sinica, 2026, 52(4): 1088-1102.
[15] Guo Xiang-Yang, Tu Liang, Wang Dong, Liu Peng-Fei, Wang An-Gui, Yi Qiang, Ren Hong, Li Gang, Zhu Yun-Fang, Wu Xun, Jiang Yu-Lin, Tian Feng, Chen Ze-Hui. Application and prospects of Suwan germplasm in maize breeding in China [J]. Acta Agronomica Sinica, 2026, 52(3): 655-664.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!