Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (5): 1273-1278.doi: 10.3724/SP.J.1006.2022.14070

• RESEARCH NOTES • Previous Articles     Next Articles

Identification of chromosome loss and rearrangement in potato and eggplant somatic hybrids by rDNA and telomere repeats

WANG Hai-Bo1,2(), YING Jing-Wen1, HE Li1,3, YE Wen-Xuan1, TU Wei1, CAI Xing-Kui1, SONG Bo-Tao1,*(), LIU Jun1   

  1. 1Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs / Key Laboratory of Horticultural Plant Biology, Ministry of Education / Potato Engineering and Technology Research Center of Hubei Province / Huazhong Agricultural University, Wuhan 430070, Hubei, China
    2College of Biological Science and Technology, Hubei Minzu University, Enshi 445000, Hubei, China
    3Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
  • Received:2021-04-20 Accepted:2021-09-10 Online:2022-05-12 Published:2021-10-18
  • Contact: SONG Bo-Tao E-mail:wanghaibo0519@126.com;songbotao@mail.hzau.edu.cn
  • Supported by:
    China Agriculture Research System (Potato, CARS-09-P07)


Somatic hybridization is an important way to create new germplasm. Somatic hybrids produced plenty of genetic variation during protoplast regeneration. In this study, to analyze the chromosome composition and variation of potato and eggplant somatic hybrids, rDNAs and telomeric repeats were used as probes for FISH (fluorescence in situ hybridization), combined with GISH (Genomic in situ hybridization). The results showed that rearranged chromosomes and dicentric chromosomes existed in somatic hybrids, and the parts of the rearranged chromosomes was derived from the end-to-end fusion of potato and eggplant chromosomes 2. One centromere of the rearranged dicentric chromosomes was derived from potato and the other was from eggplant. Eggplant 5S rDNA sites were lost in somatic hybrids to homogenize the rDNA of somatic hybrids. The results of this study indicated that the chromosomes were unstable during the somatic hybridization of potato and eggplant, which can easily cause dicentric and chromosomal rearrangements in somatic hybrids. The chromosomes of somatic hybrids tended to be stable through various ways such as chromosome rearrangement, dicentric and rDNA homogenization.

Key words: potato, eggplant, somatic hybrids, rDNA, chromosome rearrangement

Fig. 1

rDNAs locations on AC142 and 508.3 a: the distribution of rDNAs on AC142; b: the distribution of rDNAs location on 508.3. Red signals denote 5S rDNA signals, green signalsdenote 25S rDNA signals. Bar: 10 μm."

Fig. 2

Recognition of the chromosome constitution though rDNA probes and GISH in somatic hybrid derived from potato and eggplant a: recognition of chromosomes containing rDNA sites in somatic hybrid 60-13 (red signals denote 5S sites, green signals denote 25S sites); b: recognition of parental chromosome origins by GISH performed after washing (Red signals denote chromosomes of S. tuberosum. Green signals denote chromosomes of S. melongena.). Arrowheads denote 25S sites, arrows denote 5S sites. Bar: 10 μm."

Fig. 3

Recognition of the chromosome constitution though rDNA probes, telomeric probe, and GISH in somatic hybrid derived from potato and eggplant a: the recognition of chromosomes containing rDNA sites in somatic hybrid PE60-10, red blocks denote 5S rDNA sites, green blocks denote 25S rDNA sites; b: the recognition of parental chromosome origins in (a) by GISH performed after washing, red denoted chromosomes from potato, green denoted chromosomes from eggplant.; c: the distribution of telomeric probe signals in PE60-10, red denoted the telomeric sites; d: the results of GISH performed after washing telomeric probe signals, red denoted chromosomes from potato, green denoted the chromosomes from eggplant. Arrowheads denoted 25S rDNA sites; arrows denoted 5S rDNA sites. White ellipse and rounded frames denote rearrangement chromo-somes containing 25S rDNA sites. The enlarged chromosomes in Fig. a, b, c, d denote chromosomes with white frames. Bar: 10 μm."

Fig. 4

Schematic diagram showing the formation of an end-to- end fused chromosome in somatic hybrid PE60-10 The apparent lightly stained areas on chromosomes denote nucleo-lar organizing regions (NOR). Chromosomes in the picture are from parents for somatic fusion between potato and eggplant, and their somatic hybrid PE60-10."

Fig. 5

Dicentric chromosome derived from chromosomal recombination in somatic hybrid PE60-10 a and b are two chromosome spreads of somatic hybrid 60-10. Arrows denote chromosomes with two centromeres. The white frames indicate chromosomes in black and white for better observation of constrictions. White lines denote primary constrictions, yellow lines denote another apparent constriction on chromosomes, respectively. Bar: 10 μm."

[1] Xiang F N, Xia G M, Chen H M. Effect of UV dosage on somatic hybridization between common wheat (Triticum aestivum L.) and Avena sativa L. Plant Sci, 2003, 164:697-707.
doi: 10.1016/S0168-9452(03)00021-9
[2] 王晶, 向凤宁, 夏光敏, 陈惠民. 利用不对称体细胞杂交向小麦转移高冰草染色体小片段. 中国科学, 2004, 34:113-120.
Wang J, Xiang F N, Xia G M, Chen H M. Using asymmetric somatic hybridization to transfer small chromosome fragments from tall wheatgrass to wheat. Sci China, 2004, 34:113-120 (in Chinese).
[3] Greplová M, Polzerová H, Vlastníková H. Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum. Acta Physiol Plant, 2008, 30:787-796.
doi: 10.1007/s11738-008-0183-1
[4] Tarwacka J, Polkowska-Kowalczyk L, Kolano B, Kolano B, Śliwka J, Wielgat B. Interspecific somatic hybrids Solanum villosum (+) S. tuberosum, resistant to Phytophthora infestans. J Plant Physiol, 2013, 170:1541-1548.
doi: 10.1016/j.jplph.2013.06.013
[5] Fock I, Collonnier C, Purwito A, Luisetti J, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D. Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja. Plant Sci, 2000, 160:165-176.
pmid: 11164589
[6] Yu Y, Ye W X, He L, Cai X K, Liu T, Liu J. Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. Plant Cell Rep, 2013, 32:1687-1701.
doi: 10.1007/s00299-013-1480-8
[7] Austin S, Baer M A, Helgeson J P. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci, 1985, 39:75-81.
doi: 10.1016/0168-9452(85)90195-5
[8] Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T. Novel somatic hybrids (Solanum tuberosum L.+ Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet, 2008, 116:691-700.
doi: 10.1007/s00122-007-0702-2
[9] Bastia T, Carotenuto N, Basile B, Zoina A, Cardi T. Induction of novel organelle DNA variation and transfer of resistance to frost and Verticillium wilt in Solanum tuberosum through somatic hybridization with 1EBN S. commersonii. Euphytica, 2000, 116:1-10.
doi: 10.1023/A:1003943704037
[10] Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tissue Organ Cult, 2007, 91:179-189.
doi: 10.1007/s11240-007-9284-6
[11] Raina S, Rani V. GISH technology in plant genome research. Methods Cell Sci, 2001, 23:83-104.
doi: 10.1023/A:1013197705523
[12] Zluvova J, Lengerova M, Markova M, Hobza R, Nicolas M, Vyskot B, Charlesworth D, Negrutiu I, Janousek B. The inter-specific hybrid Silene latifolia × S. viscosa reveals early events of sex chromosome evolution. Evol Dev, 2005, 7:327-336.
pmid: 15982369
[13] 何礼. 茄属种及其种间体细胞杂种的染色体特征分析. 华中农业大学博士学位论文, 湖北武汉, 2013.
He L. Optimization of Potato Fluorescence in Situ Hybridization and Chromosome Characteristics of Solanum Genomes and the Inter-specific Somatic Hybrids. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2013 (in Chinese with English abstract).
[14] Wang H B, Cheng Z N, Wang B S, Dong J K, Ye W X, Yu Y, Liu T, Cai X K, Song B T, Liu J. Combining genome composition and differential gene expression analyses reveals that SmPGH1 contributes to bacterial wilt resistance in somatic hybrids. Plant Cell Rep, 2020, 39:1235-1248.
doi: 10.1007/s00299-020-02563-7
[15] Dong F, Song J, Naess S K, Helgeson J P, Gebhardt C, Jiang J. Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato. Theor AppI Genet, 2000, 101:1001-1007.
doi: 10.1007/s001220051573
[16] He L, Liu J, Torres G A, Zhang H Q, Jiang J M, Xie C H. Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species. Chromosome Res, 2012, 21:5-13.
doi: 10.1007/s10577-012-9332-x
[17] Lou Q, Iovene M, Spooner D M, Buell C R, Jiang J. Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping. Chromosoma, 2010, 119:435-442.
doi: 10.1007/s00412-010-0269-6
[18] Pendinen G, Gavrilenko T, Jiang J, Spooner D M. Allopolyploid speciation of the Mexican tetraploid potato species Solanum stoloniferum and S. hjertingii revealed by genomic in situ hybridization. Genome, 2008, 51:714-720.
doi: 10.1139/G08-052 pmid: 18772949
[19] Wu F, Tanksley S D. Chromosomal evolution in the plant family Solanaceae. BMC Genomics, 2010, 11:182.
doi: 10.1186/1471-2164-11-182
[20] Malinska H, Tate J, Matyasek R, Leitch A, Soltis D, Soltis P, Kovarik A. Similar patterns of rDNA evolution in synthetic and recently formed natural populations of Tragopogon(Asteraceae) allotetraploids. BMC Evol Biol, 2010, 10:291.
doi: 10.1186/1471-2148-10-291 pmid: 20858289
[21] Mlinarec J, Satovic Z, Malenica N, Ivancic-Bace I, Besendorfer V. Evolution of the tetraploid Anemone multifida (2n = 32) and hexaploid A. baldensis (2n = 48) (Ranunculaceae) was accompanied by rDNA loci loss and intergenomic translocation: evidence for their common genome origin. Ann Bot, 2012, 110:703-712.
doi: 10.1093/aob/mcs128
[22] Pontes O, Neves N, Silva M, Lewis M S, Madlung A, Comai L, Viegas W, Pikaard C S. Chromosomal locus rearrangements are a rapid response to formation of the allotetraploid Arabidopsis suecica genome. Proc Natl Acad Sci USA, 2004, 101:18240-18245.
doi: 10.1073/pnas.0407258102
[23] Metcalfe C J, Bulazel K V, Ferreri G C, Schroeder-Reiter E, Wanner G, Rens W, Obergfell C, Eldridge M D, O'Neill R J. Genomic instability within centromeres of interspecific marsupial hybrids. Genetics, 2007, 177:2507-2517.
doi: 10.1534/genetics.107.082313 pmid: 18073443
[24] Sato H, Masuda F, Takayama Y, Takahashi K, Saitoh S. Epigenetic inactivation and subsequent heterochromatinization of a centromere stabilize dicentric chromosomes. Curr Biol, 2012, 22:658-667.
doi: 10.1016/j.cub.2012.02.062
[1] SHI Yan-Yan, MA Zhi-Hua, WU Chun-Hua, ZHOU Yong-Jin, LI Rong. Effects of ridge tillage with film mulching in furrow on photosynthetic characteristics of potato and yield formation in dryland farming [J]. Acta Agronomica Sinica, 2022, 48(5): 1288-1297.
[2] FENG Ya, ZHU Xi, LUO Hong-Yu, LI Shi-Gui, ZHANG Ning, SI Huai-Jun. Functional analysis of StMAPK4 in response to low temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(4): 896-907.
[3] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[4] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[5] TAN Xue-Lian, GUO Tian-Wen, HU Xin-Yuan, ZHANG Ping-Liang, ZENG Jun, LIU Xiao-Wei. Characteristics of microbial community in the rhizosphere soil of continuous potato cropping in arid regions of the Loess Plateau [J]. Acta Agronomica Sinica, 2022, 48(3): 682-694.
[6] ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[7] JIAN Hong-Ju, SHANG Li-Na, JIN Zhong-Hui, DING Yi, LI Yan, WANG Ji-Chun, HU Bai-Geng, Vadim Khassanov, LYU Dian-Qiu. Genome-wide identification and characterization of PIF genes and their response to high temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 86-98.
[8] XU De-Rong, SUN Chao, BI Zhen-Zhen, QIN Tian-Yuan, WANG Yi-Hao, LI Cheng-Ju, FAN You-Fang, LIU Yin-Du, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of StDRO1 gene polymorphism and association analysis with root traits in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 76-85.
[9] ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[10] SONG Tian-Xiao, LIU Yi, RAO Li-Ping, Soviguidi Deka Reine Judesse, ZHU Guo-Peng, YANG Xin-Sun. Identification and expression analysis of cell wall invertase IbCWIN gene family members in sweet potato [J]. Acta Agronomica Sinica, 2021, 47(7): 1297-1308.
[11] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[12] LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping. Key genes mining of DNA methylation involved in regulating drought stress response in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 599-612.
[13] QIN Tian-Yuan, LIU Yu-Hui, SUN Chao, BI Zhen-Zhen, LI An-Yi, XU De-Rong, WANG Yi-Hao, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of StIgt gene family and expression profile analysis of response to drought stress in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 780-786.
[14] JIANG Wei, PAN Zhe-Chao, BAO Li-Xian, ZHOU Fu-Xian, LI Yan-Shan, SUI Qi-Jun, LI Xian-Ping. Genome-wide association analysis for late blight resistance of potato resources [J]. Acta Agronomica Sinica, 2021, 47(2): 245-261.
[15] WANG Cui-Juan, CHAI Sha-Sha, SHI Chun-Yu, ZHU Hong, TAN Zhong-Peng, JI Jie, REN Guo-Bo. Anatomy characteristics and IbEXP1 gene expression of tuberization under ammonia nitrogen treatment in sweet potato [J]. Acta Agronomica Sinica, 2021, 47(2): 305-319.
Full text



No Suggested Reading articles found!