Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 855-861.doi: 10.3724/SP.J.1006.2013.00855

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Xiaoyan 9323

TANG Ming-Shuang,MA Dong-Fang,WANG Hai-Ge,YIN Jun-Liang,HOU Lu,YAO Qiang,JING Jin-Xue*   

  1. State Key Laboratory of Crop Stress Biology for Arid Areas / College of Plant Protection, Northwest A&F University, Yangling 712100, China
  • Received:2012-08-27 Revised:2012-12-15 Online:2013-05-12 Published:2013-02-19
  • Contact: 井金学, E-mail: jingjinxue@163.com, Tel: 029-87092434

Abstract:

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Xiaoyan 9323, the homology material of Xiaoyan 6, has early maturity, strong resistance, wide adaptability, higher resistance to stripe rust, excellent biological characteristics. To identify the resistance gene(s), we crossed Xiaoyan 9323 with susceptible genotype Mingxian 169, and tested F1, F2, F3, and BC1 progenies with Chinese race CYR32 at seedling stage in greenhouse. Then, SSR techniques were used to screen molecular markers linked to the gene conferring resistance to CYR32 in Xiaoyan 9323. The genetic analysis results showed that Xiaoyan 9323 displayed resistance to epidemic yellow rust races in China when tested with five races ofstripe rust and one recessive gene conferring resistance to races CYR32. The mapping population of F2 generation was inoculated with CYR32. Six SSR markers, Xwmc807, Xbarc3, Xwmc684, Xwmc201, Xwmc553,and Xwmc179 were linked to the resistance gene, which were all located on chromosome arm 6AL. The closest flanking makers were Xwmc201 and Xwmc553 with genetic distances of 2.6 cM and 3.7 cM, respectively. According to SSR markers’ chromosome location, the gene was located at chromosome 6AL. Based on chromosomal location, reactions to various pathotypes andpedigree analysis, the gene may be a new one different from the known stripe rust resistance genes, designated as YrXY9323 temporarily. Two flanking SSR markers, Xwmc201 and Xwmc553, were used to test 42 wheat cultivars from Huang-Huai River Valleys wheat region. The results showed that 19% cultivars had the same polymorphic bands as YrXY9323, indicating that the polymorphic SSR markers can be useful in wheat molecular marker selection and breeding.

Key words: Xiaoyan 9323, Puccinia striiformis f. sp. tritici, Recessive resistance gene, Molecular mapping

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