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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 423-428.doi: 10.3724/SP.J.1006.2012.00423

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of a Rolled Leaf Gene RL13 in Rice (Oryza sativa L.)

TIAN Xiao-Qing, SANG Xian-Chun, ZHAO Fang-Ming, LI Yun-Feng, LING Ying-Hua,YANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 4007145, China
  • Received:2011-07-13 Revised:2011-10-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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Abstract: Rolled 1eaf mutants are good materials rice in breeding for leaf and plant type. A rolled 1eaf mutant rl13 was derived from an EMS-treated mutation in an indica line (Jinhui 10). The chlorophyll content of rl13 was significantly higher than that of wild type Jinhui10. There was a highly significant difference in the curl of the three functional mutant leaves compared with those with wild-type. However, there were no significant differences between the three functional mutants. Through the analysis of paraffin, mesophyll cells per layer in rl13 thinned, wild-type cells contain a large bubble into two similar size vesicular cells of mutants, which resulted in the character of rolled leaf. We made the combination of Xinong1A/rl13 to establish genetic population for genetic analysis, the results confirmed that the mutant trait was controlled by a single recessive nuclear gene. In the F2, the gene of the rolled leaf trait was mapped between two SSR markers, RM276 and SWU6-1, with distances of 1.1 cM and 0.2 cM, respectively.

Key words: Rice (Oryza sativa L.), Rolled leaf mutant, Genetic analysis, Molecular mapping

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