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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 423-428.doi: 10.3724/SP.J.1006.2012.00423

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of a Rolled Leaf Gene RL13 in Rice (Oryza sativa L.)

TIAN Xiao-Qing, SANG Xian-Chun, ZHAO Fang-Ming, LI Yun-Feng, LING Ying-Hua,YANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 4007145, China
  • Received:2011-07-13 Revised:2011-10-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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Abstract: Rolled 1eaf mutants are good materials rice in breeding for leaf and plant type. A rolled 1eaf mutant rl13 was derived from an EMS-treated mutation in an indica line (Jinhui 10). The chlorophyll content of rl13 was significantly higher than that of wild type Jinhui10. There was a highly significant difference in the curl of the three functional mutant leaves compared with those with wild-type. However, there were no significant differences between the three functional mutants. Through the analysis of paraffin, mesophyll cells per layer in rl13 thinned, wild-type cells contain a large bubble into two similar size vesicular cells of mutants, which resulted in the character of rolled leaf. We made the combination of Xinong1A/rl13 to establish genetic population for genetic analysis, the results confirmed that the mutant trait was controlled by a single recessive nuclear gene. In the F2, the gene of the rolled leaf trait was mapped between two SSR markers, RM276 and SWU6-1, with distances of 1.1 cM and 0.2 cM, respectively.

Key words: Rice (Oryza sativa L.), Rolled leaf mutant, Genetic analysis, Molecular mapping

[1]Yuan L-P(袁隆平). Breeding for super for high-yielding in hybrid rice. Hybrid Rice (杂交水稻), 1997, 12(6): 1–3 (in Chinese with English abstract)

[2]Chen W-F(陈温福), Xu Z-J(徐正进), Zhang W-Z(张文忠), Zhang L-B(张龙步), Yang S-R(杨守仁). Creation of new plant type and breeding rice for super high yield. Acta Agron Sin (作物学报), 2001, 27(5): 665–667 (in Chinese with English abstract)

[3]Chen Z-X(陈宗祥), Chen G(陈刚), Hu J(胡俊), Dai L-C(戴留春), Tao G-Y(陶国英), Pan X-B(潘学彪). Genetic expression and effects of rolled leaf gene Rl(t) in hybrid rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2002, 28(6): 847–851 (in Chinese with English abstract)

[4]Zhu D-F(朱德峰), Lin X-Q(林贤青). Comparison of leaf photosynthetic characteristics among rice hybrids with different leaf rolling index. Acta Agron Sin(作物学报), 2001, 27(3): 329–333 (in Chinese with English abstract)

[5]Shao Y-J(邵元健), Chen Z-X(陈宗祥), Zhang Y-F(张亚芳), Chen E-H(陈恩会), Qi X-CH(祁顶成), Miao J(缪进), Pan X-B(潘学彪). One major QTL mapping and physical map construction for rolled leaf in rice. Acta Genet Sin (遗传学报), 2005, 32(5): 501–506 (in Chinese with English abstract)

[6]Shi Z Y, Wang J, Wan X, Shen G, Wang X, Zhang J. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta, 2007, 226: 99–108

[7]Zhang G H, Xu Q, Zhu X D, Qian Q, Xue H W. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21: 719–735

[8]Vaucheret H, Vazquez F, Crete P, Bartel D P. The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant. Genes Dev, 2004, 43: 1187–1197

[9]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10(t), in rice (Oryza sativa L.). Genome, 2007, 50: 811–817

[10]Yan C-J(严长杰), Yan S(严松), Zhang Z-Q(张正球), Liang G-H(梁国华), Lu J-F(陆驹飞), Gu M-H(顾铭洪). Genetic analysis and gene mapping of a new rolled leaf mutant rl9(t) in rice. Chin Sci Bull (科学通报), 2006, 51(1): 2757–2762

[11]Reich P B. Effects of low concentrations of O3 on net photosynthesis, dark respiration, and chlorophyll contents in aging hybrid popular leaves. Plant Physiol, 1983, 73: 291–296

[12]Gao Y-H(高艳红), Lü C-G(吕川根), Wang M-Q(王茂青), Wang P(王澎), Yan X-Y(闫晓燕), Xie K(谢坤), Wan J-M(万建民). QTL mapping for rolled leaf gene in rice. Jiangsu J Agric Sci (江苏农业学报), 2007, 23(1): 5–10 (in Chinese with English abstract)

[13]Wang G-L(王关林), Fang H-Y(方宏筠). Plant Gene Engineering (植物基因工程), 2nd edn. Beijing: Science Press, 2002. pp 742–744 (in Chinese)

[14]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)

[15]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259: 297–607

[16]Xing G-N(邢光南), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Application technique of marker grouping and ordering in genetic linkage map construction using Mapmaker/Exp. Acta Agron Sin (作物学报), 2008, 34(2): 217–223 (in Chinese with English abstract)

[17]Lü C-G(吕川根), Zou J-S(邹江石). Comparative analysis on plant type of two super hybrid rice and Shanyou 63. Sci Agric Sin (中国农业科学), 2003, 36(6): 633–639 (in Chinese with English abstract)

[18]Lang Y-ZH(郎有忠), Zhang F-J(张祖建), Gu X-Y(顾兴友), Yang X-J(杨建昌), Zhu Q-S(朱庆森). Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.): II. Photosynthetic character, Dry mass production and yield forming. Acta Agron Sin(作物学报), 2004, 30(9): 883–887 (in Chinese with English abstract)

[19]Kinoshita T. Gene Analysis and Linkage Map. Tokyo: Japan Science Society Press, 1984. pp 187–274

[20]Khush G S, Kinoshita T. Rice karyotype, marker genes, and linkage groups. In: Khush G S, Toenniessen G H, eds. Rice Biology. Wallingford: CAB International and IRRI, 1991. pp 83–108

[21]Yu D(余东), Wu H-B(吴海滨), Yang W-T(杨文韬), Gong P-T(巩鹏涛), Li Y-Z(李有志), Zhao D-G(赵德刚). Genetic analysis and mapping of the unilateral rolled leaf trait of rice mutant B157. Mol Plant Breed (分子植物育种), 2008, 6(2): 220–226 (in Chinese with English abstract)

[22]Li S-G(李仕贵), Ma Y-Q(马玉清), He P(何平), Li H-Y(黎汉云), Chen Y(陈英), Zhou K-D(周开达), Zhu L-H(朱立煌). Genetic analysis and mapping the flag leaf roll in rice (Oryza sativa L.). J Sichuan Agric Univ (四川农业大学学报), 1998, 16(4): 391–393 (in Chinese with English abstract)

[23]Yan S, Yan C J, Zeng X H, Yang Y, Fang Y, Tian C, Sun Y, Cheng Z, Gu M. ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice. Plant Mol Biol, 2008, 68: 239–250

[24]Wang D-Z(王德仲), Sang X-C(桑贤春), You X-Q(游小庆), Wang Z(王增), Wang Q-S(王秋实), Zhao F-M(赵芳明), Ling Y-H(凌英华), Li Y-F(李云峰), He G-H(何光华). Genetic analysis and gene mapping of a novel narrow and rolled leaf mutant nrl2(t) in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2011, 37(7): 1159–1166 (in Chinese with English abstract)

[25]Luo Y-Z(罗远章), Zhao F-M(赵芳明), Sang X-C(桑贤春), Lin Y-H(凌英华), Yang Z-L(杨正林), He G-H(何光华). Genetic analysis and gene mapping of a novel rolled leaf mutant rl12(t) in rice. Acta Agron Sin (作物学报), 2009, 35(11): 1967–1972 (in Chinese with English abstract)

[26]Xia L(夏令), Chen L(陈亮), Guo C-M(郭迟鸣), Zhang H-X(张红心), Zhao Z(赵政), Shen M-S(沈明山). Genetic analysis and mapping of rice (Oryza sativa L.) sd-sl mutant. J Xiamen Univ (厦门大学学报), 2007, 46(6): 847–851 (in Chinese with English abstract)

[27]Wang D K, Liu H Q, Li K L. Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.). Chin Sci Bull, 2009, 54: 752–758
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