小麦卷叶突变体RL1的生理特性及遗传研究

doi:10.3724/SP.J.1006.2023.31004

Abstract

Abstract:

Wheat leaves tend to fold or curl when exposure to stresses, the dehydration avoidance in morphology can reduce the damage of abiotic stress. At present, the physiological and genetic regulation mechanism associated with leaf curling is not clear in wheat. This study reported a novel rolled leaf mutant (RL1) from the ethyl methyl sulphonate (EMS) mutagenesis cultivar Jinmai 47. The leaves of the mutant RL1 were curled during the growth period, and the primary leaves were slightly curled along axial vein to paraxial plane. Leaf curling was accelerated with the growth until the leaf was tubular. Compared to wild type (WT), plant height, ear length, flag leaf narrowing, and 1000-grain weight were decreased in mutant RL1. Triphenyltetrazolium Chloride (TTC) staining showed that seed vigor of RL1 was low, together with the decreased germination rate by 22%. Additionally, there was no significant differences in chlorophyll content, net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO₂ concentration between RL1 and WT, while water utilization rate was decreased in RL1 after heading for 10 days. Low temperature, high temperature, and drought led to the leaf rolling in RL1. RL1 showed fewer leaf/lobular veins via paraffin section assay, and the number of abaxial sclerenchyma and adaxial parenchyma cells were reduced in midrib region of RL1. Moreover, the area and counts of vesicular cells between the vascular bundles were significantly reduced in RL1, together with the vesicular cells at the midvein region of leaves compared with WT. Vesicular cells and vascular bundles shrunk and decreased, respectively, resulting in the situation that the entire blade was extremely crimped to the adaxial plane. Genetic analysis demonstrated that the mutant trait was localized on the short arm of chromosome 1D, regulated by a pair of nuclear genes with incomplete dominance and fine mapping analysis further locked the target interval at 9.42 Mb.

Key words: wheat, rolled leaf mutant, cytological analysis, physiological characteristics, genetic analysis

LIU Ye, LI Yue, YUAN Ming-Yang, WEI Nai-Cui, GUAN Pan-Feng, ZHAO Jia-Jia, WU Bang-Bang, ZHENG Xing-Wei, HAO Yu-Qiong, QIAO Ling, ZHENG Jun. Physiological characteristics and genetic research of rolled leaf mutant1 (RL1) in wheat (Triticum aestivum L.)[J].Acta Agronomica Sinica, 2023, 49(12): 3399-3410.

Figures/Tables 12

Table 1

Table S1

42 pairs of SSR primers (GB: NY/T 2859-2015)"

引物名称 Primer name	染色体 Chr.	退火温度 Annealing temperature (℃)	引物序列 Primer sequence (5'-3')
cwm65	1A	65	F: TCATTGGTGTCATCCCTCGTGT; R: GAATAATGCCTTGACCCTGGAC
barc80	1BL	65	F: GCGAATTAGCATCTGCATCTGTTTGAG; R: CGGTCAACCAACTACTGCACAAC
cfd72	1DL	60	F: CTCCTTGGAATCTCACCGAA; R: TCCTTGGGAATATGCCTCCT
gwm294	2AL	55	F: GGATTGGAGTTAAGAGAGAACCG; R: GCAGAGTGATCAATGCCAGA
gwm429	2BS	55	F: TTGTACATTAAGTTCCCATTA; R: TTTAAGGACCTACATGACAC
gwm261	2DS	55	F: CTCCCTGTACGCCTAAGGC; R: CTCGCGCTACTAGCCATTG
gwm155	3AL	55	F: CAATCATTTCCCCCTCCC; R: AATCATTGGAAATCCATATGCC
gwm285	3BS	65	F: ATGACCCTTCTGCCAAACAC; R: ATCGACCGGGATCTAGCC
gdm72	3DS	55	F: TGGTTTTCTCGAGCATTCAA; R: TGCAACGATGAAGACCAGAA
gwm610	4AS	65	F: CTGCCTTCTCCATGGTTTGT; R: AATGGCCAAAGGTTATGAAGG
ksum62	4B	60	F: GGAGAGGATAGGCACAGGAC; R: GAGAGCAGAGGGAGCTATGG
barc91	4DL	55	F: TTCCCATAACGCCGATAGTA; R: GCGTTTAATATTAGCTTCAAGATCAT
gwm304	5AS	55	F: AGGAAACAGAAATATCGCGG; R: AGGACTGTGGGGAATGAATG
gwm67	5BL	60	F: ACCACACAAACAAGGTAAGCG; R: CAACCCTCTTAATTTTGTTGGG
cfd29	5DL	65	F: GGTTGTCAGGCAGGATATTTG; R: TATTGATAGATCAGGGCGCA
gwm459	6AS	55	F: AATTTCAAAAAGGAGAGAGA; R: AACATGTGTTTTTAGCTATC
barc198	6BS	55	F: CGCTGAAAAGAAGTGCCGCATTATGA; R: CGCTGCCTTTTCTGGATTGCTTGTCA
cfd76	6DL	65	F: GCAATTTCACACGCGACTTA; R: CGCTCGACAACATGACACTT
cfa2028	7AS	55	F: TGGGTATGAAAGGCTGAAGG; R: ATCGCGACTATTCAACGCTT
gwm333	7BS	60	F: GCCCGGTCATGTAAAACG; R: TTTCAGTTTGCGTTAAGCTTTG
gwmn437	7DL	55	F: GATCAAGACTTTTGTATCTCTC; R: GATGTCCAACAGTTAGCTTA
wmc312	1AS	55	F: TGTGCCCGCTGGTGCGAAG; R: CCGACGCAGGTGAGCGAAG
barc240	1BL	55	F: AGAGGACGCTGAGAACTTTAGAGAA; R: GCGATCTTTGTAATGCATGGTGAAC
gdm111	1DL	55	F: CACTCACCCCAAACCAAAGT; R: GATGCAATCGGGTCGTTAGT
wmc522	2AS	55	F: AAAAATCTCACGAGTOGGGC; R: CCCGAGCAGGAGCTACAAAT
cfd51	2DS	55	F: GGAGGCTTCTCTATGGGAGG; R: TGCATCTTATCCTGTGCAGC
barc324	3AS	55	F: CCAATTCTGCCCATAGGTGA; R: GAGGAAATAAGATTCAGCCAACTG
barc164	3BS	55	F: TGCAAACTAATCACCAGCGTAA; R: CGCTTTCTAAAACTGTTCGGGATTTCTAA
cfd9	3DL	55	F: TTGCACGCACCTAAACTCTG; R: CAAGTGTGAGCGTCGG
gwm161	3DS	55	F: GATCGAGTGATGGCAGATGG; R: TGTGAATTACTTGGACGTGG
barc170	4AL	55	F: CGCTTGACTTTGAATGGCTGAACA; R: CGCCCACTTTTTACCTAATCCTTTTGAA
gwm495	4BL	55	F: GAGAGCCTCGCGAAATATAGG; R: TGCTTCTGGTGTTCCTTCG
winc720	4DS	55	F: CACCATGGTTGGCAAGAGA; R: CTGGTGATACTGCCGTGACA
gwm186	5AL	55	F: GCAGAGCCTGGTTCAAAAAG; R: CGCCTCTAGCGAGAGCTATG
cfa2155	5AL	55	F: TTTGTTACAACCCAGGGGG; R: TTGTGTGGCGAAAGAAACAG
cfd8	5DS	60	F: ACCACCGTCATGTCACTGAG; R: GTGAAGACGACAAGACGCAA
gwml69	6AL	55	F: ACCACTGCAGAGAACACATACG; R: GTGCTCTGCTCTAAGTGTGGG
barc345	6BL	55	F: CGCCAGACTGCTAGGATAATACTTT; R: GCGGCTAGTGCTCCCTCATAAT
barcl121	6DL	60	F: GCGAGCAAACTGATCCCAAAAAG; R: TATCGGTGAGTACGCCAAAAACA
cfa2123	7AS	60	F: CGGTCTTTGTTTGCTCTAAACC; R: ACCGGCCATCTATGATGAAG
wmc476	7BS	55	F: TACCAACCACACCTGCGAGT; R: CTAGATGAACCTTCGTGCGG
gwm44	7DS	65	F: GTTGAGCTTTTCAGTTCGGC; R: ACTGGCATCCACTGAGCTG

Table S1

Fig. 1

Fig. 2

Table 2

Fig. 3

Fig. 4

Fig. 5

Table S2

Table 3

Fig. 6

Table 4

Homologous genes of rice leaf curl related genes in wheat"

水稻基因 Rice gene	基因注释 Gene annotation	部分同源群 Homoeologous groups	小麦的同源基因 Wheat gene
OsACL1	Os04g33860	2	TraesCS2A01G296600 TraesCS2B01G312800 TraesCS2D01G294500
OsDEK1	Os02g47970	6	TraesCS6A01G275500 TraesCS6B01G302900 TraesCS6D01G255800
OsCFL1	Os02g31140	4	TraesCS4A01G407300 TraesCS4B01G306500 TraesCS4D01G304700
OsMYB103L	Os08g05520	7	TraesCS7A01G304500 TraesCS7B01G204800 TraesCS7D01G300000
OsVIL3	Os02g05840	6	TraesCS6A02G123400 TraesCS6B02G151600 TraesCS6D02G113600
OsAGO7	Os03g33650	2	TraesCS2A01G414800 TraesCS2B01G434000 TraesCS2D01G412100
OsCOW1	Os03g06654	4	TraesCS4A01G027500 TraesCS4B01G278300 TraesCS4D01G276600
OsCSLD4	Os12g36890	5	TraesCS5A01G090500 TraesCS5B01G096200 TraesCS5D01G102600
OsHOX32	Os03g43930	5	TraesCS5A01G375800 TraesCS5B01G378000 TraesCS5D01G385300
OsYAB1	Os07g06620	5	TraesCS5A01G371500 TraesCS5B01G373600 TraesCS5D01G380900
OsZHD1	Os09g29130	5	TraesCS5A01G246500 TraesCS5B01G243800 TraesCS5D01G253100
OsPSL1	Os01g19170	1	TraesCS1A01G311100 TraesCS1B01G322500 TraesCS1D01G311000
OsREL1	Os01g64380	2	TraesCS2A01G065100 TraesCS2B01G077300 TraesCS2D01G063200
OsREL2	Os10g41310	1	TraesCS1A01G218200 TraesCS1B01G231600 TraesCS1D01G219800
Osrl14	Os10g40960	1	TraesCS1A01G221200 TraesCS1B01G234500 TraesCS1D01G222800
OsROC5	Os02g45250	6	TraesCS6A01G255800 TraesCS6B01G269700 TraesCS6D01G237000
OsRoc8	Os06g10600	6	TraesCS6A01G324500 TraesCS6B01G354900 TraesCS6D01G304300
OsSLL1	Os09g23200	5	TraesCS5A01G203200 TraesCS5B01G201900 TraesCS5D01G209600
OsSRL2	Os03g19520	4	TraesCS4A01G121600 TraesCS4B01G181700 TraesCS4D01G184300
OsSrl1	Os07g01240	2	TraesCS2A01G256400 TraesCS2B01G286100 TraesCS2D01G347200
OsOFP1	Os01g12690	3	TraesCS3A01G151800 TraesCS3B01G178800 TraesCS3D01G159700
OsMKB3	Os03g52320	4	TraesCS4A01G250600 TraesCS4B01G064000 TraesCS4D01G062900
OsLBD3-7	Os03g57670	5	TraesCS5A01G515300 TraesCS5B01G282700 TraesCS5D01G291300
OsARVL4	Os04g33580	1	TraesCS1A01G036100 TraesCS1B01G231600 TraesCS1D01G219800
OsARF18	Os06g47150	7	TraesCS7A01G446900 TraesCS7B01G346700 TraesCS7D01G436800
OsWOX3A	Os12g01120	5	TraesCS5A01G157300 TraesCS5B01G156400 TraesCS5D01G162600

Table 4

References 46

[1]	Bogard M, Hourcade D, Piquemal B, Gouache D, Deswartes J C, Throude M, Cohan J P. Marker-based crop model-assisted ideotype design to improve avoidance of abiotic stress in bread wheat. J Exp Bot, 2021, 72: 1085-1103. doi: 10.1093/jxb/eraa477 pmid: 33068400
[2]	Merrium S, Ali Z, Tahir M H N, Habib-Ur-Rahman M, Hakeem S. Leaf rolling dynamics for atmospheric moisture harvesting in wheat plant as an adaptation to arid environments. Environ Sci Pollut Res Int, 2022, 29: 48995-49006. doi: 10.1007/s11356-022-18936-2
[3]	Sirault X R R, Condon A G, Wood J T, Farquhar G D, Rebetzke G J. ‘Rolled-upness’: phenotyping leaf rolling in cereals using computer vision and functional data analysis approaches. Plant Methods, 2015, 11: 52. doi: 10.1186/s13007-015-0095-1 pmid: 26583042
[4]	Zhang X Y, Jia H Y, Li T, Wu J Z, Nagarajan R, Lei L, Powers C, Kan C C, Hua W, Liu Z Y, Chen C, Carver B F, Yan L L. TaCol-B5 modifies spike architecture and enhances grain yield in wheat. Science, 2022, 376: 180-183. doi: 10.1126/science.abm0717
[5]	Sun J, Cui X A, Teng S Z, Zhao K N, Wang Y W, Chen Z H, Sun X H, Wu J X, Ai P F, Quick W P, Lu T G, Zhang Z G. HD-ZIP IV gene Roc8 regulates the size of bulliform cells and lignin content in rice. Plant Biotechnol J, 2020, 18: 2559-2572. doi: 10.1111/pbi.v18.12
[6]	Li L, Shi Z Y, Li L, Shen G Z, Wang X Q, An L S, Zhang J L. Overexpression of ACL1 (abaxially curled leaf 1) increased bulliform cells and induced abaxial curling of leaf blades in rice. Mol Plant, 2010, 3: 807-817. doi: 10.1093/mp/ssq022
[7]	Zou L P, Sun X H, Zhang Z G, Liu P, Wu J X, Tian C J, Qiu J L, Lu T G. Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice. Plant Physiol, 2011, 156: 1589-1602. doi: 10.1104/pp.111.176016
[8]	Zhang G H, Xu Q, Zhu X D, Qian Q, Xue H W. SHALLOT- LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21: 719-735. doi: 10.1105/tpc.108.061457
[9]	邹良平. 水稻卷叶突变体的细胞形成机制以及OUL1基因的克隆和功能研究. 中国农业科学院博士学位论文,北京, 2012.
	Zou L P. Cytological Mechanism of Rolled-feaf Formation and Functional Analysis of OUL1 Controlling Leaf Roll in Rice. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2012. (in Chinese with English abstract)
[10]	Wu R H, Li S B, He S, Wassmann F, Yu C H, Qin G J, Schreiber L, Qu L J, Gu H Y. CFL1, a WW domain protein, regulates cuticle development by modulating the function of HDG1, a class IV homeodomain transcription factor, in rice and Arabidopsis. Plant Cell, 2011, 23: 3392-3411. doi: 10.1105/tpc.111.088625
[11]	Juarez M T, Twigg R W, Timmermans M C P. Specification of adaxial cell fate during maize leaf development. Development, 2004, 131: 4533-4544. doi: 10.1242/dev.01328 pmid: 15342478
[12]	Canales C, Grigg S, Tsiantis M. The formation and patterning of leaves: recent advances. Planta, 2005, 221: 752-756. pmid: 15909148
[13]	Zhu Z, Wang J Y, Li C N, Li L, Mao X G, Hu G, Wang J P, Chang J Z, Jing R L. A transcription factor TaMYB5 modulates leaf rolling in wheat. Front Plant Sci, 2022, 13: 897623. doi: 10.3389/fpls.2022.897623
[14]	Verma A, Niranjana M, Jha S K, Mallick N, Agarwal P, Vinod. QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat. Sci Rep, 2020, 10: 18696. doi: 10.1038/s41598-020-75703-4 pmid: 33122772
[15]	Yang X, Wang J Y, Mao X G, Li C N, Li L, Xue Y H, He L H, Jing R L. A locus controlling leaf rolling degree in wheat under drought stress identified by bulked segregant analysis. Plants, 2022, 11: 2076. doi: 10.3390/plants11162076
[16]	Bian R L, Liu N, Xu Y Z, Su Z Q, Chai L L, Bernardo A, Amand P St, Fritz A, Zhang G R, Rupp J, Akhunov E, Jordan K W, Bai G H. Quantitative trait loci for rolled leaf in a wheat EMS mutant from Jagger. Theor Appl Genet, 2023, 136: 52. doi: 10.1007/s00122-023-04284-3
[17]	赵佳佳, 乔玲, 武棒棒, 葛川, 乔麟轶, 张树伟, 闫素仙, 郑兴卫, 郑军. 山西省小麦苗期根系性状及抗旱特性分析. 作物学报, 2021, 47: 714-727. doi: 10.3724/SP.J.1006.2021.01048
	Zhao J J, Qiao L, Wu B B, Ge C, Qiao L Y, Zhang S W, Yan S X, Zheng X W, Zheng J. Seedling root characteristics and drought resistance of wheat in Shanxi province. Acta Agron Sin, 2021, 47: 714-727. (in Chinese with English abstract) doi: 10.3724/SP.J.1006.2021.01048
[18]	李浩然, 李慧玲, 王红光, 李东晓, 李瑞奇, 李雁鸣. 冬小麦叶面积测算方法的再探讨. 麦类作物学报, 2018, 38: 455-459.
	Li H R, Li H L, Wang H G, Li X D, Li R Q, Li Y M. Further study on the method of leaf area calculation in winter wheat. J Triticeae Crops, 2018, 38: 455-459. (in Chinese with English abstract)
[19]	Shi Z Y, Wang J, Wan X S, Shen G Z, Wang X Q, Zhang J L. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta, 2007, 226: 99-108. doi: 10.1007/s00425-006-0472-0
[20]	Selim D A H, Zayed M, Ali M M E, Eldesouky H S, Bonfill M, El-Tahan A M, Ibrahim O M, El-Saadony M T, El-Tarabily K A, AbuQamar S F, Elokkiah S. Germination, physio-anatomical behavior, and productivity of wheat plants irrigated with magnetically treated seawater. Front Plant Sci, 2022, 13: 923872. doi: 10.3389/fpls.2022.923872
[21]	张礼霞, 刘合芹, 于新, 王林友, 范宏环, 金庆生, 王建军. 水稻卷叶突变体rl15(t)的生理学分析及基因定位. 中国农业科学, 2014, 47: 2881-2888. doi: 10.3864/j.issn.0578-1752.2014.14.018
	Zhang L X, Liu H Q, Yu X, Wang L Y, Fan H H, Jin Q S, Wang J J. Molecular mapping and physiological characterization of a novel mutant rl15(t) in rice. Sci Agric Sin, 2014, 47: 2881-2888. (in Chinese with English abstract) doi: 10.3864/j.issn.0578-1752.2014.14.018
[22]	严长杰, 严松, 张正球, 梁国华, 陆驹飞, 顾铭洪. 一个新的水稻卷叶突变体rl9(t)的遗传分析和基因定位. 科学通报, 2005, 50: 2757-2762.
	Yan C J, Yan S, Zhang Z Q, Liang G H, Lu J F, Gu M H. Genetic analysis and gene fine mapping for a rice novel mutant rl9(t) with rolling leaf character. Sci Bull, 2005, 50: 2757-2762. (in Chinese with English abstract)
[23]	Duan P G, Ni S, Wang J M, Zhang B L, Xu R, Wang Y X, Chen H Q, Zhu X D, Li Y H. Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice. Nat Plants, 2015, 2: 15203. doi: 10.1038/nplants.2015.203
[24]	Li Y Y, Shen A, Xiong W, Sun Q L, Luo Q, Song T, Li Z L, Luan W J. Overexpression of OsHox32 results in pleiotropic effects on plant type architecture and leaf development in rice. Rice, 2016, 9: 46. doi: 10.1186/s12284-016-0118-1
[25]	Liu X F, Li M, Liu K, Tang D, Sun M F, Li Y F, Shen Y, Du G J, Cheng Z K. Semi-Rolled Leaf2 modulates rice leaf rolling by regulating abaxial side cell differentiation. J Exp Bot, 2016, 67: 2139-2150. doi: 10.1093/jxb/erw029
[26]	Shimano S, Hibara K I, Furuya T, Arimura S I, Tsukaya H, Itoh J I. Conserved functional control, but distinct regulation, of cell proliferation in rice and Arabidopsis leaves revealed by comparative analysis of GRF-INTERACTING FACTOR 1 orthologs. Development, 2018, 145: 159624.
[27]	Jane W N, Chiang S H T. Morphology and development of bulliform cells in Arundo formosana Hack. Taiwania Int J Life Sci, 1991, 36: 85-97.
[28]	Hibara K L, Obara M, Hayashida E, Abe M, Ishimaru T, Satoh H, Itoh J L, Nagato Y. The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice. Dev Biol, 2009, 334: 345-354. doi: 10.1016/j.ydbio.2009.07.042
[29]	Xu Y, Wang Y H, Long Q Z, Huang J X, Wang Y L, Zhou K N, Zheng M, Sun J, Chen H, Chen S H, Jiang L, Wang C M, Wan J M. Overexpression of OsZHD1, a zinc finger homeodomain class homeobox transcription factor, induces abaxially curled and drooping leaf in rice. Planta, 2014, 239: 803-816. doi: 10.1007/s00425-013-2009-7
[30]	Li C, Zou X H, Zhang C Y, Shao Q H, Liu J, Liu B, Li H Y, Zhao T. OsLBD3-7 overexpression induced adaxially rolled leaves in rice. PLoS One, 2016, 11: e0156413. doi: 10.1371/journal.pone.0156413
[31]	Yang C H, Li D Y, Liu X, Ji C J, Hao L L, Zhao X F, Li X B, Chen C Y, Cheng Z K, Zhu L H. OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.). BMC Plant Biol, 2014, 14: 158. doi: 10.1186/1471-2229-14-158
[32]	Kinoshita T. Gene analysis and linkage map. Tokyo: Japan Scientific Societies Press, 1984. pp 187-274.
[33]	Khush G S, Kinoshita T. Rice karyotype, marker genes, and linkage groups. In: Khush G S, Toenniessen G H, eds. Rice Biology. Wallingford: CAB International and International Rice Research Institute, 1991. pp 83-108.
[34]	Wang J, Hu J, Qian Q, Xue H W. LC2 and OsVIL2 promote rice flowering by photoperoid-induced epigenetic silencing of OsLF. Mol Plant, 2013, 6: 514-527. doi: 10.1093/mp/sss096 pmid: 22973062
[35]	Woo Y M, Park H J, Su'udi M, Yang J I, Park J J, Back K, Park Y M, An G. Constitutively wilted 1, a member of the rice YUCCA gene family, is required for maintaining water homeostasis and an appropriate root to shoot ratio. Plant Mol Biol, 2007, 65: 125-136. doi: 10.1007/s11103-007-9203-6
[36]	Hu J, Zhu L, Zeng D L, Gao Z Y, Guo L B, Fang Y X, Zhang G Z, Dong G J, Yan M X, Liu J, Qian Q. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol, 2010, 73: 283-292. doi: 10.1007/s11103-010-9614-7
[37]	Dai M Q, Zhao Y, Ma Q, Hu Y F, Hedden P, Zhang Q F, Zhou D X. The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism. Plant Physiol, 2007, 144: 121-133.
[38]	Zhang G H, Hou X, Wang L, Xu J, Chen J, Fu X, Shen N W, Nian J Q, Jiang Z Z, Hu J, Zhu L, Rao Y C, Shi Y F, Ren D Y, Dong G J, Gao Z Y, Guo L B, Qian Q, Luan S. PHOTO- SENSITIVE LEAF ROLLING 1 encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice. New Phytol, 2021, 229: 890-901. doi: 10.1111/nph.v229.2
[39]	Chen Q L, Xie Q J, Gao J, Wang W Y, Sun B, Liu B H, Zhu H T, Peng H F, Zhao H B, Liu C H, Wang J, Zhang J L, Zhang G Q, Zhang Z M. Characterization of Rolled and Erect Leaf 1 in regulating leave morphology in rice. J Exp Bot, 2015, 66: 6047-6058. doi: 10.1093/jxb/erv319
[40]	Yang S Q, Li W Q, Miao H, Gan P F, Qiao L, Chang Y L, Shi C H, Chen K M. REL2, a gene encoding an unknown function protein which contains DUF630 and DUF632 domains controls leaf rolling in rice. Rice, 2016, 9: 37. doi: 10.1186/s12284-016-0105-6
[41]	Fang L K, Zhao F M, Cong Y F, Sang X C, Du Q, Wang D Z, Li Y F, Ling Y H, Yang Z L, He G H. Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves. Plant Biotechnol J, 2012, 10: 524-532. doi: 10.1111/pbi.2012.10.issue-5
[42]	Xiang J J, Zhang G H, Qian Q, Xue H W. Semi-rolled leaf1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells. Plant Physiol, 2012, 159: 1488-1500. doi: 10.1104/pp.112.199968
[43]	Xiao Y H, Liu D P, Zhang G X, Tong H N, Chu C C. Brassinosteroids regulate OFP1, a DLT interacting protein, to modulate plant architecture and grain morphology in rice. Front Plant Sci, 2017, 8: 1698. doi: 10.3389/fpls.2017.01698 pmid: 29021808
[44]	Wang L, Xu J, Nian J Q, Shen N W, Lai K K, Hu J, Zeng D L, Ge C W, Fang Y X, Zhu L, Qian Q, Zhang G G. Characterization and fine mapping of the rice gene OsARVL4 regulating leaf morphology and leaf vein development. Plant Growth Regul, 2016, 78: 345-356. doi: 10.1007/s10725-015-0097-z
[45]	Huang J, Li Z Y, Zhao D Z. Deregulation of the OsmiR160 target gene OsARF18 causes growth and developmental defects with an alteration of auxin signaling in rice. Sci Rep, 2016, 6: 29938. doi: 10.1038/srep29938 pmid: 27444058
[46]	Cho S H, Yoo S C, Zhang H T, Pandeya D, Koh H J, Wang J Y, Kim G T, Paek N C. The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol, 2013, 198: 1071-1084. doi: 10.1111/nph.2013.198.issue-4

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Li Shaoqing, Li Yangsheng, Wu Fushun, Liao Jianglin, Li Damo. Optimum Fertilization and Its Corresponding Mechanism under Complete Submergence at Booting Stage in Rice[J]. Acta Agronomica Sinica, 2002, 28(01): 115 -120 .
[2]	Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[3]	YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[4]	Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[5]	Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[6]	WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[7]	TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[8]	HU Xi-Yuan;LI Jian-Ping;SONG Xi-Fang. Efficiency of Spatial Statistical Analysis in Superior Genotype Selection of Plant Breeding[J]. Acta Agron Sin, 2008, 34(03): 412 -417 .
[9]	ZHENG Xi;WU Jian-Guo;LOU Xiang-Yang;XU Hai-Ming;SHI Chun-Hai. Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments[J]. Acta Agron Sin, 2008, 34(03): 369 -375 .
[10]	XING Guang-Nan, ZHOU Bin, ZHAO Tuan-Jie, YU De-Yue, XING Han, HEN Shou-Yi, GAI Jun-Yi. Mapping QTLs of Resistance to Megacota cribraria (Fabricius) in Soybean[J]. Acta Agronomica Sinica, 2008, 34(03): 361 -368 .

标记 Marker	SNP	F₁	F₂	R
RLB	T/C	CCAACCCTAGCTTGTGATCCC	TCCAACCCTAGCTTGTGATCCT	AGGGGCCCACAAGCCTCCTG
RLC	C/G	GAATCACGAAAGCACCCTCGC	GAATCACGAAAGCACCCTCGG	GGCAAACGTGTAGATCGTCTCCC
RLF	A/C	CGTAACATACAACCATGCCTACCT	GTAACATACAACCATGCCTACCG	CGTTCAAGGGTTCACATAAGGCTTTTG
RLH	A/C	GTGGCATGTCAACTCGCGATTTATA	GGCATGTCAACTCGCGATTTATC	CAACTGAGTGGCATATATATAGTCTGTTATA
RLI	A/C	CAGAATCACTTCTTGACTACCCCAA	GAATCACTTCTTGACTACCCCAC	CAGCCTTCTAGAGCCACATTCTAGTA
RLJ	T/C	GTGCTCCTAGGAAGTAGGAGC	ATGTGCTCCTAGGAAGTAGGAGT	CCAAAAGCAAGGACGCTTCCAGG
RLK	T/C	TATTTTGAGACGAAGAGAGAATCGTG	ATTTATTTTGAGACGAAGAGAGAATCGTA	AGGCGGGTGCTTGCCCTTACAT
RLO	C/G	TTATACTAAAGCTGCATCAATTAACTTGC	TTATACTAAAGCTGCATCAATTAACTTGG	GGGCAGTACAACAAACATCAGAAATAAC

性状Trait	种子萌发15 d地上部鲜重 Aboveground fresh weight at 15 days of germination (g)	种子萌发15 d地下部鲜重 Subsurface fresh weight at 15 days of germination (g)	种子萌发15 d 根数目 Root number at 15 days of germination	种子萌发15 d 最大根长 Maximum root length at 15 days of germination (cm)	抽穗期 Heading stage (d)	成熟期 Maturity stage (d)	株高 Plant height (cm)	旗叶面积 Flag leaf area (cm²)	旗叶长 Flag leaf length (cm)	旗叶宽 Flag leaf width (cm)	穗粒数 Grain number per spike	小穗数 Spikelet number	千粒重 1000-grain weight (g)
WT	0.22±0.01^**	0.08±0.01	7±1.3	8.13±0.84	199±1	248±1	78.00±0.28^**	24.62±5.14	21.02±2.65	1.51±0.12^**	56.75±6.65^**	19.25±1.25	52.25±0.91^**
RL1	0.14±0.00	0.08±0.01	7±1.5	8.08±1.26	199±1	247±1	67.67±2.19	19.72±2.51	23.4±2.45	1.09±0.02	35.5±1.91	19.00±0.81	33.70±0.21

杂交组合 Cross combination	F₁表型及卷曲指数 Phenotype of F₁ and leaf rolled index	F₁株数 Number of plants in F₁
RL1×晋麦47 RL1/Jinmai 47	微卷(抽穗期LRIs: 0.64-0.76) Slightly curl (Heading date LRIs: 0.64-0.76)	17
晋麦47×RL1 Jinmai 47/RL1	微卷(抽穗期LRIs: 0.66-0.78) Slightly curl (Heading date LRIs: 0.66-0.78)	18
RL1×临汾5064 RL1/Linfen 5064	微卷(抽穗期LRIs: 0.61-0.76) Slightly curl (Heading date LRIs: 0.61-0.76)	17
临汾5064×RL1 Linfen 5064/RL1	微卷(抽穗期LRIs: 0.68-0.80) Slightly curl (Heading date LRIs: 0.68-0.80)	18

杂交组合 Cross combination	总株数 Number of plants	平展叶 Spread leaf	轻微卷叶 Slightly coiled leaf	高度卷叶 Highly rolled leaf	理论比例 Ratio of theory	卡方 χ²
RL1/晋麦47 RL1/Jinmai 47	176	45	94	37	1:2:1	0.03
RL1/临汾5064 RL1/Linfen 5064	173	44	95	34	1:2:1	0.01

Physiological characteristics and genetic research of rolled leaf mutant1 (RL1) in wheat (Triticum aestivum L.)

RichHTML418

PDF

Abstract

Cite this article

share this article

Figures/Tables 12

References 46

Related Articles 15

Metrics

Comments

Recommended 10

[1]	LI Yu-Jia, XU Hao, YU Shi-Nan, TANG Jian-Wei, LI Qiao-Yun, GAO Yan, ZHENG Ji-Zhou, DONG Chun-Hao, YUAN Yu-Hao, ZHENG Tian-Cun, YIN Gui-Hong. Genetic analysis of elite stripe rust resistance genes of founder parent Zhou8425B in its derived varieties [J]. Acta Agronomica Sinica, 2024, 50(1): 16-31.
[2]	ZHANG Li-Hua, ZHANG Jing-Ting, DONG Zhi-Qiang, HOU Wan-Bin, ZHAI Li-Chao, YAO Yan-Rong, LYU Li-Hua, ZHAO Yi-An, JIA Xiu-Ling. Effect of water management on yield and its components of winter wheat in different precipitation years [J]. Acta Agronomica Sinica, 2023, 49(9): 2539-2551.
[3]	ZHANG Diao-Liang, YANG Zhao, HU Fa-Long, YIN Wen, CHAI Qiang, FAN Zhi-Long. Effects of multiple cropping green manure on grain quality and yield of wheat with different irrigation levels [J]. Acta Agronomica Sinica, 2023, 49(9): 2572-2581.
[4]	SU Zai-Xing, HUANG Zhong-Qin, GAO Run-Fei, ZHU Xue-Cheng, WANG Bo, CHANG Yong, LI Xiao-Shan, DING Zhen-Qian, YI Yuan. Identification of wheat dwarf mutant Xu1801 and analysis of its dwarfing effect [J]. Acta Agronomica Sinica, 2023, 49(8): 2133-2143.
[5]	YANG Xiao-Hui, WANG Bi-Sheng, SUN Xiao-Lu, HOU Jin-Jin, XU Meng-Jie, WANG Zhi-Jun, FANG Quan-Xiao. Modeling the response of winter wheat to deficit drip irrigation for optimizing irrigation schedule [J]. Acta Agronomica Sinica, 2023, 49(8): 2196-2209.
[6]	LI Yu-Xing, MA Liang-Liang, ZHANG Yue, QIN Bo-Ya, ZHANG Wen-Jing, MA Shang-Yu, HUANG Zheng-Lai, FAN Yong-Hui. Effects of exogenous trehalose on physiological characteristics and yield of wheat flag leaves under high temperature stress at grain filling stage [J]. Acta Agronomica Sinica, 2023, 49(8): 2210-2224.
[7]	LIU Qiong, YANG Hong-Kun, CHEN Yan-Qi, WU Dong-Ming, HUANG Xiu-Lan, FAN Gao-Qiong. Effect of nitrogen application rate on grain quality, wine quality and volatile flavor compounds of waxy and no-waxy wheat [J]. Acta Agronomica Sinica, 2023, 49(8): 2240-2258.
[8]	LIN Fen-Fang, CHEN Xing-Yu, ZHOU Wei-Xun, WANG Qian, ZHANG Dong-Yan. Hyperspectral remote sensing detection of Fusarium head blight in wheat based on the stacked sparse auto-encoder algorithm [J]. Acta Agronomica Sinica, 2023, 49(8): 2275-2287.
[9]	LIU Shi-Jie, YANG Xi-Wen, MA Geng, FENG Hao-Xiang, HAN Zhi-Dong, HAN Xiao-Jie, ZHANG Xiao-Yan, HE De-Xian, MA Dong-Yun, XIE Ying-Xin, WANG Li-Fang, WANG Chen-Yang. Effects of water and nitrogen application on root characteristics and nitrogen utilization in winter wheat [J]. Acta Agronomica Sinica, 2023, 49(8): 2296-2307.
[10]	CHEN Li, WANG Jing, QIU Xiao, SUN Hai-Lian, ZHANG Wen-Hao, WANG Tian-Zuo. Differences of physiological responses and transcriptional regulation of alfalfa with different drought tolerances under drought stresses [J]. Acta Agronomica Sinica, 2023, 49(8): 2122-2132.
[11]	DING Hong-Yan, FENG Xiao-Xi, WANG Bai-Yu, ZHANG Ji-Sen. Evolution and relative expression pattern of LRRII-RLK gene family in sugarcane Saccharum spontaneum [J]. Acta Agronomica Sinica, 2023, 49(7): 1769-1784.
[12]	ZHANG Zhen, SHI Yu, ZHANG Yong-Li, YU Zhen-Wen, WANG Xi-Zhi. Effects of different soil water content on water consumption by wheat and analysis of senescence characteristics of root and flag leaf [J]. Acta Agronomica Sinica, 2023, 49(7): 1895-1905.
[13]	ZHANG Lu-Lu, ZHANG Xue-Mei, MU Wen-Yan, HUANG Ning, GUO Zi-Kang, LUO Yi-Nuo, WEI Lei, SUN Li-Qian, WANG Xing-Shu, SHI Mei, WANG Zhao-Hui. Grain Mn concentration of wheat in main wheat production regions of China: Effects of cultivars and soil factors [J]. Acta Agronomica Sinica, 2023, 49(7): 1906-1918.
[14]	DONG Zhi-Qiang, LYU Li-Hua, YAO Yan-Rong, ZHANG Jing-Ting, ZHANG Li-Hua, YAO Hai-Po, SHEN Hai-Ping, JIA Xiu-Ling. Yield and quality of strong gluten wheat Shiluan 02-1 under water and nitrogen interaction [J]. Acta Agronomica Sinica, 2023, 49(7): 1942-1953.
[15]	LI Ling-Yu, ZHOU Qi-Rui, LI Yang, ZHANG An-Min, WANG Bei-Bei, MA Shang-Yu, FAN Yong-Hui, HUANG Zheng-Lai, ZHANG Wen-Jing. Transcriptome analysis of exogenous 6-BA in regulating young spike development of wheat after low temperature at booting stage [J]. Acta Agronomica Sinica, 2023, 49(7): 1808-1817.