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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (03): 429-435.doi: 10.3724/SP.J.1006.2012.00429

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of a Short Root-Related Gene OsKSR2 in Rice (Oryza sativa L.)

LUO Li-Li,SHI Jun-Ying,XIANG Xian-Bo,DING Wo-Na*,ZHU Shi-Hua   

  1. Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China
  • Received:2011-07-11 Revised:2011-10-15 Online:2012-03-12 Published:2012-01-04
  • Contact: 丁沃娜, E-mail: dwn@zju.edu.cn

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Abstract: Theroot system of plant plays an important role in supporting plants and uptaking nutrients and water in soil. In this study, a rice mutant with significantly short roots was isolated from an EMS (ethyl methane sulfonate)-generated mutant library of Kasalath and named as Osksr2(Oryza sativa kasalath short root 2). Osksr2 showed a dwarf phenotype and the elongation of primary roots, adventitious roots and lateral roots in the mutant was severely impaired. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene, and named as OsKSR2. To map OsKSR2, we generated an F2 population by crossing Osksr2 with Nipponbare wild type. By using the published SSR markers and newly designed STS markers, OsKSR2 was mapped to a 101 kb region between the markers S27887 and S27988 on chr.8. Within this region there were seventeen predicted genes, and none of them had been reported to be involved in root development. The study will be helpful for the cloning of OsKSR2 and characterization of the molecular mechanism of root elongation in rice.

Key words: Rice (Oryza sativa L.), Short root mutant, Gene mapping, Genetic analysis

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