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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 862-867.doi: 10.3724/SP.J.1006.2013.00862

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Mapping of Rice Early Senescent Leaf (esl3) Mutant

MIAO Run-Long,JIANG Yu-Dong,LIAO Hong-Xiang,XU Fang-Fang,HE Guang-Hua,YANG Zheng-Lin,ZHAO Fang-Ming,SANG Xian-Chun*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Received:2012-10-12 Revised:2012-12-15 Online:2013-05-12 Published:2013-02-19
  • Contact: 桑贤春, E-mail: sangxianchun@163.com

Abstract:

Leaf early senescence can directly decrease crop photosynthesis, yield and quality. Therefore, the identifications of mutants with early senescence and its gene function play key roles in crop genetic improvement. A mutant with early senescent leaf blades, named as esl3, was identified from a EMS-induced progeny in restorer line of Jinhui 10. The middle-upper leaf in mutation displayed a brown and withered phenotype from seedling stage to maturity. Correspondingly, the chlorophyll content and net photosynthetic rate declined in the abnormal leaves while enhanced in the normal green part of leaves compared with the wild type. Agronomic traits, such as panicle number, panicle length, grains per panicle, 1000-grain weight, plant height and weight of dry matter, were reduced significantly or very significantly except for seed setting rate as compared with those of wild type. Genetic analysis showed the senescence was controlled by a recessive nuclear gene. Using 391 mutation individuals in a F2 generation of Nippomese/esl3, ESL3 was mapped between SSR marker RM19085 and Indel marker Ind05-2 on the chromosome 5 with physical distance 91 kb including 14 annotated genes. All of these results provide a foundation for the further gene cloning and functional analysis of ESL3 gene.

Key words: Rice (Oryza sativa), Early senescence, Gene mapping, Photosynthetic rate

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