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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1173-1180.doi: 10.3724/SP.J.1006.2009.01173


Identification and Molecular Mapping of Xa32(t),a Novel Resistance Gene for Bacterial Blight(Xanthomonas oryzae pv.oryzae)in Rice

ZHENG Chong-Ke1,2,**,WANG Chun-Lian2,3,**, YU Yuan-Jie1, LIANG Yun-Tao2,ZHAO Kai-Jun23*   

  1. 1College of Agronomy,Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai'an 271018,China;2Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China
  • Received:2009-01-21 Revised:2009-03-13 Online:2009-07-12 Published:2009-05-18
  • Contact: ZHAO Kai-Jun,E-mail:zhaokj@mail.caas.net.cn


The rice bacterial blight (BB), caused by Xanthmonas oryzae pv. oryzae (Xoo),is the most devastating bacterial disease of rice worldwide. Use of resistant varieties has been thought the most economical and environment-friendly approach to control BB of rice. Since the rapid changes of the pathogenicity of the pathogen (Xoo), new BB resistance genes are always needed for rice breeding. We, here, report the identification and molecular mapping of a new BB resistance gene from Oryzae ustraliensis. Based on the resistance spectrum analysis, we identified a new rice germplasm, C4064, for bacterial blight (BB) resistance. Leaf-cutting innoculation assays showed that C4064 was resistant to Xoo strains P1 (PXO61), P4 (PXO71), P5 (PXO112), P6 (PXO99), P7 (PXO145), P8 (PXO280),P9 (PXO339), and KX085, but susceptible to P2 (PXO86) and P3 (PXO79). Comparison of resistance spectrum with that of all the known BB resistance genes and genetic analysis revealed that the rice germplasm C4064harbored a new BB-resistance gene, designated as Xa32(t). To molecularly map Xa32(t) gene,Bulked Segregant Analysis (BSA) was adopted to survey SSR and EST molecular markers. Out of 299 markers tested, six markers RM27256, RM27274, RM2064, ZCK24, RM6293, and RM5926 on rice chromosome 11 displayed polymorphism between the S-pool and R-pool. By analyzing the F2 and F3 populations, the gene Xa32 (t) was mapped on the long arm of rice chromosome 11. Linkage analysis revealed that molecular markers RM27256, RM27274, RM2064, and ZCK24 were located between Xa32 (t) and the centromere of the chromosome, with genetic distances of 2.1, 1.0, 1.0, and 0.5 cM to Xa32 (t), respectively; while RM6293 and RM5926 were located onthe othersideof Xa32(t), with genetic distances of 1.5 and 2.6 cM to Xa32 (t), respectively. Thus, the new BB-resistance gene Xa32(t) was mapped within a length of 2.0 cM on the long arm of rice chromosome 11. The results of this study will be useful in fine mapping of Xa32(t) and marker-assisted breeding for BB resistant rice varieties.

Key words: Rice bacterial blight, Resistance spectrum analysis, Xa32(t), Molecular mapping

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