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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1405-1409.doi: 10.3724/SP.J.1006.2009.01405


Genetic Analysis and Molecular Mapping of a Yellow green Leaf Gene(YGL4) in Rice (Oryza sativa L.)

LIU Meng-Meng,SANG Xian-Chun,LING Ying-Hua,DU Peng,ZHAO Fang-Ming,YANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Key Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Chongqing 400716, China
  • Received:2008-11-22 Revised:2009-02-17 Online:2009-08-12 Published:2009-06-10
  • Contact: HE Guang-Hua,E-mail: hegh@swu.edu.cn


A leaf color mutant was obtained by EMS treating seeds of restorer line Jinhui 10, this mutation showed complete yellow green leaves during the life, and could be regenerated and inherited stably according to the observation of 5 generations. The content of its total chlorophyll ranged from 2.01 to 2.28 mg g-1, which was only 38.2% to 50.5% of the original parent. Compared with the original parent, the mutation had no significant difference in the traits of main panicle length, first branch number, filled grain number of main panicle, seed setting rate and 1000-grain weight, except the effective panicle and plant height which were decreased significantly. Genetic analysis of F2 populations confirmed that the mutational character was controlled by a single recessive nuclear gene, temporarily designated as YGL4. The gene was mapped between two microsatellite markers RM3123 and RM590, with genetic distances of 7.6 cM and 7.8 cM to the two markers respectively. New microsatellite markers were designed between RM3123 and RM590, and the YGL4 gene was final mapped between RM1162 and RM7093, with genetic distances of 1.8 cM and 4.0 cM to each of them respectively. This result provided a foundation of molecular marker-assisted breeding and map-based cloning of YGL4 gene.

Key words: Rice(Oryza sativa L.), Yellow green Leaf, Genetic Analysis, Molecular Mapping

[1] Leister D. Chloroplast research in the genomic age. Trends Genet, 2003, 19: 47-56

[2] Mochizuki N, Brusslan J A, Larkin R, Nagatani A, Chory J. Arabidopsis genomes uncoupled 5(GUN5) mutant reveals the involvement of mg-chelatase H subunit in plastid-to-nucleus signal transduction. Proc Natl Acad Sci USA, 2001, 98: 2053-2058

[3] Robert M L, Jose M A, Joseph R E. GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science, 2003, 299: 902-906

[4] Stern D B, Hanson M R, Barkan A. Genetics and genomics of chloroplast biogenesis: Maize as a model system. Trends Plant Sci, 2004, 9: 293-301

[5] Beale S. Green genes gleaned. Trends Plant Sci, 2005, 10: 309-312

[6] Dong F-G(董凤高), Zhu X-D(朱旭东), Xiong Z-M(熊阵民), Cheng X-H(程式华), Sun Z-X(孙宗修), Min S-K(闵绍楷). Breeding of a photo-thermoperiod sensitive genie male sterile indica rice with a pale-green-leaf marker. Chin Rice Sci (中国水稻科学), 1995, 9(2): 65-70 (in Chinese with English abstract)

[7] Wu D X, Shu Q Y, Xia Y W. In vitro mutagenesis induced novel thermo photoperiod sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker. Euphytica, 2002, 123: 195-202

[8] Yutaka S, Ryouhei M, Minoru N, Hiroyasu Y, Makoto K.Mendel’s green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway. Proc Natl Acad Sci USA, 2007, 104: 14169-14174

[9] Gong H-B(龚红兵), Chen L-M (陈亮明), Diao L-P(刁立平), Sheng S-L(盛生兰), Lin T-Z(林添资), Yang T-N(杨图南), Zhang R-X(张荣铣), Cao S-Q(曹树青), Zhai H-Q(翟虎渠), Dai X-B(戴新宾), Lu W(陆巍), Xu X-M(许晓明). Genetic analysis of chlorophyll-b less mutant in rice and its related characteristics. Sci Agric Sin (中国农业科学), 2001, 34(6): 686-689 (in Chinese with English abstract)

[10] ABE T, Matsuyama T, Sekido S, Yamaguchi I, Yoshida S, Kameya T. Chlorophyll deficient mutants of rice demonstrated the deletion of a DNA fragment by heavy-ion irradiation. J Radiat Res, 2002, 43: 157-161

[11] Wang C, He B, Xun M, Wan J. Tagging and mapping of a gene controlling yellowish-green leaf. Rice Genet Newslett, 2003, 20: 35

[12] Wang J(王军), Wang B-H(王宝和), Zhou L-H(周丽慧), Xu J-F(徐洁芬), Gu M-H(顾铭洪), Liang G-H(梁国华). Genetic analysis and molecular mapping of a new yellow-green leaf gene ygl-2 in rice. Chin Rice Sci (中国水稻科学), 2006, 20: 455-459(in Chinese with English abstract)

[13] Huang X-Q(黄晓群), Wang P-R(王平荣), Zhao H-X(赵海新), Dong X-J(邓晓建). Genetic analysis and molecular mapping of a novel chlorophyll-deficit mutant gene in rice. Chin Rice Sci (中国水稻科学), 2007, 21(4): 355-359 (in Chinese with English abstract)

[14] Jung K H, Hur J, Ryu C H,Choi Y, Chung Y Y, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463-472

[15] Lee S, Kim J H, Enu S Y, Lee C H, Hirochika H, Gynheung A. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57: 805-818

[16] Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M.A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29-40

[17] Lichtenthaler H K. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol, 1987, 48: 350-382

[18] Wang G-L(王关林), Fang H-Y(方宏筠). Plant Gene Engineering (植物基因工程), 2nd edn. Beijing: Science Press, 2002. pp 742-744 (in Chinese)

[19] Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705-707 (in Chinese with English abstract)

[20] Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259: 297-607

[21] Ayumi T, Ryouichi T. Chlorophyll metabolism. Curr Opin Plant Biol, 2006, 9: 248-255

[22] Hirochika H, Guiderdoni E, An G, Hsing Y, Moo Y E, Han C D, Upadhyaya N, Ramachandran S, Zhang Q F, Pereira A, Sundaresan V, Heilenug. Rice mutant resources for gene discovery. Plant Mol Biol, 2004, 54: 325-334

[23] He B(何冰), Liu L-L(刘玲珑), Zhang W-W(张文伟), Wan J-M(万建民). Plant leaf color mutants. Plant Physiol Commun (植物生理学通讯), 2006, 42(1): 1-9 (in Chinese with English abstract)

[24] Dong Y J, Dong W Q, Shi S Y, Jin Q. Identification and genetic analysis of a thermo sensitive seeding colour mutant in rice. Breed Sci, 2001, 51: 1-4

[25] Liu Y-J(刘友杰) Chlorotic mutants induced by laser He-Ne and its genetic expression. Appl Laser (应用激光), 1988, 8(4): 177-187 (in Chinese with English abstract)

[26] Iwata N, Satoh H, Omura T. Studies on the trisomics in rice plants (Oryza sativa L.): VII. On some maker genes located on chromosome 2 and their sequence on the linkage map. J Fac Agric Kyushu Univ, 1984, 29: 139-144

[27] Iwata N, Omura T. Studies on the trisomics in rice plants (Oryza sativa L.): III. Relation between trisomics and genetic linkage groups. Jpn J Breed, 1975, 25: 363-368

[28] Zhang H, Li J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Paek N C. Rice chlorine-1 and chlorine-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 6: 325-337

[29] Morita R, Kusaba M, Yamaguchi H, Amano E, Miyao A, Hirochika H, Nishimura M. Characterization of chlorophyllide a oxygenase (CAO) in rice. Breed Sci, 2005, 55: 361-364

[30] Koumoto Y, Shimada T, Kondo M, Nishimura I H, Nishimura M. Chloroplasts Have a Novel Cpn10 in Addition to Cpn20 as Co-chaperonins in Arabidopsis thaliana. J Biol Chem, 2001, 276: 29688-29694
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