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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1410-1417.doi: 10.3724/SP.J.1006.2009.01410

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of cDNAs for a Noval Sugar Transporter Gene,ZmERD6,from Maize and Its Expression Analysis under Abiotic Stresses

MA Xiao-Long1,2, LIU Ying-Hui2,3,**, YUAN Zu-Li1,*, SHI Yun-Su2, SONG Yan-Chun2, WANG Tian-Yu2, and LI Yu2,*   

  1. 1 College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Hebei North University, Zhangjiakou 075000, China
  • Received:2009-02-16 Revised:2009-04-22 Online:2009-08-12 Published:2009-06-10
  • Contact: LI Yu，E-mail: yuli@mail.caas.net.cn；YUAN Zu-Li，E-mail: zuliyuan@yahoo.com.cn

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Abstract:

Drought is one of the most important limiting factors for crop yield in the majority of agricultural regions around the world. Plants respond to drought stress at physiological, cellular and molecular levels. Carbohydrate substances as a nutrient and signal substances play an important role in plants throughout the life course. Carbohydrate has a variety of functions, such as providing energy for the cell life, a framework for proteins and nucleic acid molecules, and raw materials for new cells, regulating osmotically in plants to enhance tolerance, activating different signal transduction pathways and inhibition or activation of certain plant genes which regulate many physiological processes. Previous studies reported that ERD6 is a sugar transporter which widely exists in plants, belonging to Major Facilitator Super-family (MFS) which has a typical structure of MFS domain and sugar transport proteins signature. An EST with high similarity to ERD6 in Arabidopsis was found previously in maize. In the present study cDNAs for the gene homologous to ERD6, designated ZmERD6, was obtained through in silico and homology-based cloning techniques. ZmERD6 had two transcripts, i.e. ZmERD6-L (the large one) and ZmERD6-S (the small one). ZmERD6-L had an ORF of 1 515 bp and encoded 505 amino acids (AA) while ZmERD6-S had an ORF of 1 386 bp and encoded 463 AA. The deduced protein of ZmERD6-L and ZmERD6-S was predicted to contain 12 and 11 membrane spanning helices, respectively. Both of the two proteins had two MFS structural domain belonging to the sugar transporter sub-family of the MFS. Reverse transcription-PCR analysis was performed to investigate the expression pattern of the ZmERD6 in maize under various abiotic stresses. ZmERD6 was expressed at different stages through maize development and was also induced by different abiotic stresses. The promoter of ZmERD6 was cloned, which was about 2.5 kb upstream of ZmERD6 and was predicted to contain important regulatory elements including core promoter elements, enhancer elements, repressor elements and low-temperature and MeJA-responsive elements. These results suggest that the gene is a novel sugar transporter gene in maize and has important and diverse roles in tolerance to abiotic stresses.

Key words: ZmERD6, Sugar transporter, Promoter, Gene expression, Zea mays

[1] Taji T, Seki M, Yamaguchi-Shinozaki K, Kamada H, Giraudat J, Shinozaki K. Mapping of 25 drought-inducible genes, RD and ERD, in Arabidopsis thaliana. Plant Cell Physiol, 1999, 40: 119-123
[2] Kyiosue T, Shinozaki K. Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidapsis thaliana L. FEBS, 1993, 335: 189-192
[3] Kiyosue T, Abe H, Yamaguchi-Shinozaki K, Shinozaki K. ERD6, a cDNA clone for an early dehydration-induced gene of Arabidopsis, encodes a putative sugar transporter. Biochem Biophys Acta /Biomembranes, 1998, 1370: 187-191
[4] Chiou T J, Bush D R. Sucrose is a signal molecule in assimilate partitioning. Proc Natl Acad Sci USA, 1998, 95: 4784-4788
[5] Sinha A K, Hofmann M G. Metabolizable and non-metabolizable sugars activate different signal transduction pathways in tomato. Plant Physiol, 2002, 128: 1480-1489
[6] Riesmeier J W, Willmitzer L, Frommer W B. Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO J, 1992, 11: 4705-4713
[7] Sauer N, Stolz J. SUC1 and SUC2: Two sucrose transporters from Arabidopsis thaliana. Expression and characterization in baker’s yeast and identification of the histidine-tagged protein. Plant J, 1994, 6: 67-77
[8] Kuhn C, Quick W P, Schulz A, Riesmeier J W, Sonnewald U, Frommer W B. Companion cell-specific inhibition of the potato sucrose transporter SUT1. Plant Cell Environ, 1996, 19: 1115-1123
[9] Gahrtz M, Stolz J, Sauer N A. phloem-specific sucrose-H1 symporter from Plantago major L. supports the model of apoplastic phloem loading. Plant J, 1994, 6: 697-706
[10] Hirose T, Imaizumi N, Scofield G N, Furbank R T, Ohsugi R. cDNA cloning and tissue specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol, 1997, 38: 1389-1396
[11] Scofield G N, Aoki N, Hirose T, Takano M, Jenkins C L D, Furbank R T. The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants. J Exp Bot, 2007, 58: 483-495
[12] Aoki N, Whitfeld P, Hoeren F, Scofield G, Newell K, Patrick J, Offler C, Clarke B, Rahman S, Furbank R T. Three sucrose transporter genes are expressed in the developing grain of hexaploid wheat. Plant Mol Biol, 2002, 50: 453-462
[13] Aoki N, Hirose T, Takahashi S, Ono K, Ishimaru K, Ohsugi R. Molecular cloning and expression analysis of a gene for a sucrose transporter in maize (Zea mays L.). Plant Cell Physiol, 1999, 40: 1072-1078
[14]Aoki N, Hirose T, Scofield G N, Whitfeld P R, Furbank R T. The sucrose transporter gene family in rice. Plant Cell Physiol, 2003, 44:223-232
[15] Braun D M, Slewinski T L. Genetic control of carbon partitioning in grasses: roles of sucrose transporters and Tie-dyed loci in phloem loading. Plant Physiol, 2009, 149: 71-81
[16] Barker L, Kuhn C, Weise A, Schulz A, Gebhardt C, Hirner B, Hellmann H, Schulze W, Ward J M, Frommer W B. SUT2, a putative sucrose sensor in sieve elements. Plant Cell, 2000, 12: 1153-1164
[17] Gottwald J R, Krysan P J, Young J C, Evert R F, Sussman M R. Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters. Proc Natl Acad Sci USA, 2000, 97: 13979-13984
[18] Li H Y, Wang T Y, Shi Y S, Fu J J, Song Y C, Wang G Y, Li Y. Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). DNA Seq, 2007, 18: 445-460
[19] Sandra N, Oliver, Elizabeth S, Dennis, Rudy D. ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice. Plant Cell Physiol, 2007, 48: 1319-1330
[20] Singh K B. Transcriptional regulation in plants: The importance of combinatorial control. Plant Physiol, 1998, 118: 1111-1120
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