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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1410-1417.doi: 10.3724/SP.J.1006.2009.01410

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of cDNAs for a Noval Sugar Transporter Gene,ZmERD6,from Maize and Its Expression Analysis under Abiotic Stresses

MA Xiao-Long1,2, LIU Ying-Hui2,3,**, YUAN Zu-Li1,*, SHI Yun-Su2, SONG Yan-Chun2, WANG Tian-Yu2, and LI Yu2,*   

  1. 1 College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Hebei North University, Zhangjiakou 075000, China
  • Received:2009-02-16 Revised:2009-04-22 Online:2009-08-12 Published:2009-06-10
  • Contact: LI Yu，E-mail: yuli@mail.caas.net.cn；YUAN Zu-Li，E-mail: zuliyuan@yahoo.com.cn

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Abstract:

Drought is one of the most important limiting factors for crop yield in the majority of agricultural regions around the world. Plants respond to drought stress at physiological, cellular and molecular levels. Carbohydrate substances as a nutrient and signal substances play an important role in plants throughout the life course. Carbohydrate has a variety of functions, such as providing energy for the cell life, a framework for proteins and nucleic acid molecules, and raw materials for new cells, regulating osmotically in plants to enhance tolerance, activating different signal transduction pathways and inhibition or activation of certain plant genes which regulate many physiological processes. Previous studies reported that ERD6 is a sugar transporter which widely exists in plants, belonging to Major Facilitator Super-family (MFS) which has a typical structure of MFS domain and sugar transport proteins signature. An EST with high similarity to ERD6 in Arabidopsis was found previously in maize. In the present study cDNAs for the gene homologous to ERD6, designated ZmERD6, was obtained through in silico and homology-based cloning techniques. ZmERD6 had two transcripts, i.e. ZmERD6-L (the large one) and ZmERD6-S (the small one). ZmERD6-L had an ORF of 1 515 bp and encoded 505 amino acids (AA) while ZmERD6-S had an ORF of 1 386 bp and encoded 463 AA. The deduced protein of ZmERD6-L and ZmERD6-S was predicted to contain 12 and 11 membrane spanning helices, respectively. Both of the two proteins had two MFS structural domain belonging to the sugar transporter sub-family of the MFS. Reverse transcription-PCR analysis was performed to investigate the expression pattern of the ZmERD6 in maize under various abiotic stresses. ZmERD6 was expressed at different stages through maize development and was also induced by different abiotic stresses. The promoter of ZmERD6 was cloned, which was about 2.5 kb upstream of ZmERD6 and was predicted to contain important regulatory elements including core promoter elements, enhancer elements, repressor elements and low-temperature and MeJA-responsive elements. These results suggest that the gene is a novel sugar transporter gene in maize and has important and diverse roles in tolerance to abiotic stresses.

Key words: ZmERD6, Sugar transporter, Promoter, Gene expression, Zea mays

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