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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1181-1187.doi: 10.3724/SP.J.1006.2009.01181


Cloning and Analysis of a Salt Stress Related Gene TaMYB32 in Wheat

ZHANG Li-Chao,ZHAO Guang-Yao,JIA Ji-Zeng,KONG Xiu-Ying*   

  1. Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture/Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences Beijing 100081,China
  • Received:2009-02-06 Revised:2009-03-14 Online:2009-07-12 Published:2009-05-18
  • Contact: KONG Xiu-Jing,E-mail:xykong@mail.caas.net.cn


Transcriptional factors play an important role in plant adapt ability to abiotic stress at molecular level. MYB transcriptional factor family is a multifunctional gene family that have been found some of them take part in response to plant abiotic stress.In the large-scale sequencing of the wheat full length cDNAs cloned in our laboratory and functional analysising of transcriptional factors, a salt stress related gene was screened out and named TaMYB32. TaMYB32 is 1 250 bp in full length with a 732 bp ORF, encoding a R2R3-MYB transcriptional factor with 244 amino acids. The sequences of TaMYB32 were cloned from the diploid ancestors of Triticum urartu UR206, Aegilops speltoides Y2006 and Aegilops tauschii Y2282 and hexaploid wheat of Chinese Spring and Chadianhong using the primers designed based on the cDNA sequence of TaMYB32. Sequence analysis indicated that two types of sequences existed in the diploid ancestors and four in hexaploid wheat. One of the sequences was the same in the diploid and hexaploid wheats which implied that TaMYB32 was very conservative during the evolution of wheat. After comparing the genomic sequences with their cDNA sequences of TaMYB32, we found that it was a non-intron gene. TaMYB32 was mapped onto wheat homoeologous group 6 using electronic mapping strategy; there were two copies in each genome of hexaploid wheat, which was consistent with the sequencing results. Homologous analysis found thatTaMYB32 had a similarity with R2R3-MYB proteins from rice and maize as high as 72.4% and 73.7%, respectively. Tissue specific analysis indicated that TaMYB32 expressed in root, stem, leaf, pistil and anther. Semi-quantitative and real-time RT-PCR revealed that the expression of TaMYB32 was induced by salt stress.

Key words: Wheat, MYB transcriptional factor, Salt-tolerance related gene

[1]Nakashima K, Ito Y, Yamaguchi-Shinozaki K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol, 2009, 149: 88-95

[2]Yamaguchi-Shinozaki K. Gene networks involved in drought stress response and tolerance. J Exp Bot, 2007, 58: 221-227

[3]Kreps J A, Wu Y, Chang H S, Zhu T, Wang X, Harper J. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol, 2002, 130: 2129-2141

[4]Seki M, Narusaka M, Ishida J. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J, 2002, 31: 279-292

[5]Pabo C O, Sauer R T. Transcription factors: structural families and principles of DNA recognition. Annu Rev Biochem, 1992, 61: 1053-1095

[6]Riechmann J L, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe O J, Samaha R R, Creelman R, Pilgrim M, Broun P, Zhang J Z, Ghandehari D, Sherman B K, Yu G. Arabidopsistranscription factors: Genome-wide comparative analysis among eukaryotes. Science, 2000, 290: 2105-2110

[7]Rosinski J A,Atchley W R. Molecular evolution of the Myb family of transcription factors: Evidence for polyphyletic origin. Mol Evol, 1998, 46: 74-83

[8]Jin H,Martin C. Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol, 1999, 41: 577-585

[9]Ogata K, Morikawa S, Nakamura H, Hojo H, Yoshimura S, Zhang R, Aimolo S, Ametani Y, Hirata Z, Sarai A, Ishii S,Nishimura Y. Comparison of the free and DNA-complexed forms of the DNA-binding domain of c-Myb. Nat Struct Biol, 2005, 2: 309-320

[10]Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kana H, Sarai A, Ishii S, Nishimura Y. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices. Cell, 1997, 79: 639-648

[11]Urao T, Yamaguchi-Shinozaki K, Urao S, Shinozaki K. An Arabidopsis MYB homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell, 1993, 5: 1529-1539

[12]Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15: 63-78

[13]Cominelli E, Sala T, Calvi D, Gusmaroli G, Tonelli C. Over-expression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability. Plant J, 2008, 53: 53-64

[14]Jung C K, Seo J S, Han S W, Koo Y, Kim C H, Song S I, Nahm B H, Choi Y D, Cheong J. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol, 2008, 146: 623-635

[15]Denekamp M, Smeekens S C. Integration of wounding and osmotic stress signals determines the expression of the AtMYB102 transcription factor gene. Plant Physiol, 2003, 132: 1415-1423

[16]Vannini C, Locatelli F, Bracale M, Magnani E, Marsoni M, Osnato M, Mattana M, Baldoni E, Coraggio I.Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants. Plant J, 2004, 37: 115-127

[17]Dai X, Xu Y, Ma Q, Xu W, Wang T, Xue Y, Chong K. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiol, 2007, 143: 1739-1751

[18]Lee T G, Jang C S, Kim J Y, Kim D S, Park J H, Kim D Y, Seo Y W. A Mybtranscription factor (TaMyb1) from wheat roots is expressed during hypoxia: roles in response to the oxygen concentration in root environment and abiotic stresses. Physiol Plant, 2007, 129: 375-385

[19]Qi L L, Echalier B, Chao S, Lazo G R, Butler G E, Anderson O D, Akhunov E D, Dvorák J, Linkiewicz A M, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis C E, Greene R A, Kantety R, La Rota C M, Munkvold J D, Sorrells S F, Sorrells M E, Dilbirligi M, Sidhu D, Erayman M, Randhawa H S, Sandhu D, Bondareva S N, Gill K S, Mahmoud A A, Ma X F, Miftahudin, Gustafson J P, Conley E J, Nduati V, Gonzalez-Hernandez J L, Anderson J A, Peng J H, Lapitan N L V, Hossain K G, Kalavacharla V, Kianian S F, Pathan M S, Zhang D S, Nguyen H T, Choi D W, Fenton R D, Close T J, McGuire P E, Qualset C O, Gill B S. A chromosome bin map of 16 000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics, 2004, 168: 701-712

[20]Randhawa H S, Dilbirligi M, Sidhu D, Erayman M, Sandhu D, Bondareva S, Chao S, Lazo G R, Anderson O D, Miftahudin Gustafson J P, Echalier B, Qi L L, Gill B S, Akhunov E D, Dvorák J, Linkiewicz A M, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis C E, Greene R A, Sorrells M E, Conley E J, Anderson J A, Peng J H, Lapitan N L V, Hossain K G, Kalavacharla V, Kianian S F, Pathan M S, Nguyen H T, Endo T R, Close T J, McGuire P E, Qualset C O, Gill K S. Deletion mapping of homoeologous group 6-specific wheat expressed sequence tags. Genetics, 2004, 168: 677-686

[21]Akhunov E D, Goodyear A W, Geng S, Qi L L, Echalier B, Gill B S, Miftahudin Gustafson J P, Lazo G, Chao S M, Anderson O D, Linkiewicz A M, Dubcovsky J, La Rota M, Sorrells M E, Zhang D S, Nguyen H T, Kalavacharla V, Hossain K, Kianian S F, Peng J H, Lapitan N L V, Gonzalez-Hernandeiz J L, Anderson J A, Choi D W, Close T J, Dilbirligi M, Gill K S, Walker-Simmons M K, Steber C, McGuire P E, Qualset C O, Dvorak J. The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms.Genome Res, 2003, 13: 753-763

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