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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1181-1187.doi: 10.3724/SP.J.1006.2009.01181

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis of a Salt Stress Related Gene TaMYB32 in Wheat

ZHANG Li-Chao,ZHAO Guang-Yao,JIA Ji-Zeng,KONG Xiu-Ying*   

  1. Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture/Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences Beijing 100081,China
  • Received:2009-02-06 Revised:2009-03-14 Online:2009-07-12 Published:2009-05-18
  • Contact: KONG Xiu-Jing,E-mail:xykong@mail.caas.net.cn

Abstract:

Transcriptional factors play an important role in plant adapt ability to abiotic stress at molecular level. MYB transcriptional factor family is a multifunctional gene family that have been found some of them take part in response to plant abiotic stress.In the large-scale sequencing of the wheat full length cDNAs cloned in our laboratory and functional analysising of transcriptional factors, a salt stress related gene was screened out and named TaMYB32. TaMYB32 is 1 250 bp in full length with a 732 bp ORF, encoding a R2R3-MYB transcriptional factor with 244 amino acids. The sequences of TaMYB32 were cloned from the diploid ancestors of Triticum urartu UR206, Aegilops speltoides Y2006 and Aegilops tauschii Y2282 and hexaploid wheat of Chinese Spring and Chadianhong using the primers designed based on the cDNA sequence of TaMYB32. Sequence analysis indicated that two types of sequences existed in the diploid ancestors and four in hexaploid wheat. One of the sequences was the same in the diploid and hexaploid wheats which implied that TaMYB32 was very conservative during the evolution of wheat. After comparing the genomic sequences with their cDNA sequences of TaMYB32, we found that it was a non-intron gene. TaMYB32 was mapped onto wheat homoeologous group 6 using electronic mapping strategy; there were two copies in each genome of hexaploid wheat, which was consistent with the sequencing results. Homologous analysis found thatTaMYB32 had a similarity with R2R3-MYB proteins from rice and maize as high as 72.4% and 73.7%, respectively. Tissue specific analysis indicated that TaMYB32 expressed in root, stem, leaf, pistil and anther. Semi-quantitative and real-time RT-PCR revealed that the expression of TaMYB32 was induced by salt stress.

Key words: Wheat, MYB transcriptional factor, Salt-tolerance related gene

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