应用基因芯片分析红蚰麦白粉菌胁迫条件下的基因表达谱

doi:10.3724/SP.J.1006.2009.01188

Abstract

Abstract:

Wheat landrace Hongyoumai carries one dominant resistance gene for powdery mildew (Blumeria graminis), tentatively designated Pmhym. To further elucidate molecular mechanism of the resistance to powdery mildew in Hongyoumai, a resistant pool was constructed with four homogenous resistant lines from the F₃ generation of Yumai 13/Hongyoumai. The wheat seedlings were inoculated with single spore isolates of B. graminis f. sp. tritici, and at 0 and 24 h after inoculation the gene expression profile was analyzed using Affymetrix wheat microarray. There were approximately 5 282 expressed genes (with Log ratio ≥ 2 or ≤ 0.5) among total 61 127 genes set in the microarray plate, including 2 553 up-regulated and 2729 down-regulated genes or expressed sequence tags (ESTs). In the up-regulated ESTs whose functions have been known, 39.81% were disease/defense genes. In the down-regulated ESTs, energy genes and disease/defense genes accounted for 26.71% and 19.65%, respectively. A total of 81 ESTs were up-regulated more than eight times, in which most were related to disease resistance, such as pathogenesis-related protein, defense genes, genes producing or eliminating reactive oxygen species, and genes involved in signal transduction. Quantitative RT-PCR (qRT-PCR) was carried out with 13 probe sets up-regulated and down-regulated more than eight times which validated a good reproducibility of microarray analysis.

Key words: Wheat, Powdery mildew resistance gene, Gene chip, Expressed sequence Tag（EST）, Real-time quantitative RT-PCR(q-PCR)

WANG Jun-Mei,LIU Hong-Yan,XU Hong-Ming,WANG Fei,GAO Su-Xia,. Expression Profile of Landrace Hongyoumai Infected by Blumeria graminis f.sp.tritici Using Gene Microarray[J].Acta Agron Sin, 2009, 35(7): 1188-1193.

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Expression Profile of Landrace Hongyoumai Infected by Blumeria graminis f.sp.tritici Using Gene Microarray

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