应用基因芯片分析红蚰麦白粉菌胁迫条件下的基因表达谱

doi:10.3724/SP.J.1006.2009.01188

Abstract

Abstract:

Wheat landrace Hongyoumai carries one dominant resistance gene for powdery mildew (Blumeria graminis), tentatively designated Pmhym. To further elucidate molecular mechanism of the resistance to powdery mildew in Hongyoumai, a resistant pool was constructed with four homogenous resistant lines from the F₃ generation of Yumai 13/Hongyoumai. The wheat seedlings were inoculated with single spore isolates of B. graminis f. sp. tritici, and at 0 and 24 h after inoculation the gene expression profile was analyzed using Affymetrix wheat microarray. There were approximately 5 282 expressed genes (with Log ratio ≥ 2 or ≤ 0.5) among total 61 127 genes set in the microarray plate, including 2 553 up-regulated and 2729 down-regulated genes or expressed sequence tags (ESTs). In the up-regulated ESTs whose functions have been known, 39.81% were disease/defense genes. In the down-regulated ESTs, energy genes and disease/defense genes accounted for 26.71% and 19.65%, respectively. A total of 81 ESTs were up-regulated more than eight times, in which most were related to disease resistance, such as pathogenesis-related protein, defense genes, genes producing or eliminating reactive oxygen species, and genes involved in signal transduction. Quantitative RT-PCR (qRT-PCR) was carried out with 13 probe sets up-regulated and down-regulated more than eight times which validated a good reproducibility of microarray analysis.

Key words: Wheat, Powdery mildew resistance gene, Gene chip, Expressed sequence Tag（EST）, Real-time quantitative RT-PCR(q-PCR)

WANG Jun-Mei,LIU Hong-Yan,XU Hong-Ming,WANG Fei,GAO Su-Xia,. Expression Profile of Landrace Hongyoumai Infected by Blumeria graminis f.sp.tritici Using Gene Microarray[J].Acta Agron Sin, 2009, 35(7): 1188-1193.

References

[1] Han J, Lee H, Nguyen N Y, Beaucage S L, Puri R K. Novel multiple 5'-amino-modified primer for DNA microarrays. Genomics, 2005, 86: 252-258

[2] Veerasingham S J, Sellers K W, Raizada M K. Functional genomics as an emerging strategy for the investigation of central mechanisms in experimental hypertension. Prog Biophys Mol Biol, 2004, 84: 107-123

[3] Wang X-F(王锡锋), Zhang Z-S(张忠山), Liu H-Y(刘红彦), He W-L(何文兰). Evaluation of resistance and slow-mildewing of some wheat varieties in Henan Province. Acta Agric Univ Henanensis (河南农业大学学报), 1996, 30(2): 160-163 (in Chinese with English abstract)

[4] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method. Methods, 2001, 25: 402-408

[5] Bevan M, Bancroft I, Bent E, LoveK, GoodmanH,DeanC, Bergkamp R, DirkseW,Van StaverenM, Stiekema W,DrostL, RidleyP,Hudson S A,PatelK, Murphy G, PiffanelliP, WedlerH, WedlerE, WambuttR, WeitzeneggerT, PohlT M, TerrynN, GielenJ, VillarroelR, De ClerckR, Van MontaguM, LecharnyA, AuborgS, Gy I, KreisM, LaoN, Kavanagh T, Hempel S, KotterP, Entian K D, Rieger M, Schaeffer M, Funk B, Mueller-Auer S, Silvey M, James R, Montfort A, PonsA, PuigdomenechP, DoukaA, Voukelatou E, MilioniD, Hatzopoulos P, PiravandiE, ObermaierB, HilbertH, Düsterhöft A, Moores T, Jones J D G, Eneva T, Palme K, Benes V, RechmanS, AnsorgeW, CookeR, Berger C, Delseny M, Voet M, Volckaert G, Mewes H W, Klosterman S, Schueller C, Chalwatzis N. Analysis of l.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature, 1998, 391: 485-488

[6] Li A L, Wang M L, Zhou R H, Kong X Y, Huo N X, Wang W S, Jia J Z. Comparative analysis of early H₂O₂ accumulation in compatible and incompatible wheat powdery mildew interaction. Plant Pathol, 2005, 54: 308-316

[7] Luo M(骆蒙), Kong X-Y(孔秀英), Huo N-X(霍纳新), Zhou R-H(周荣华), Jia J-Z(贾继增). ESTs analysis of resistance to powdery mildew in wheat at primary infected stage. Acta Genet Sin (遗传学报), 2002, 29(6): 525-530 (in Chinese with English abstract)

[8] Cao A-Z(曹爱忠), Li Q(李巧), Chen Y-P(陈雅平), Zou X-W(邹晓文), Wang X-E(王秀娥), Chen P-D(陈佩度). Screening resistance-related genes to powdery mildew in Haynaldia villosa using barley genechip and studying its mechanism of resistance. Acta Agron Sin (作物学报), 2006, 32(10): 1444-1452 (in Chinese with English abstract)

[9] Selitrennikoff C P. Antifungal proteins.Appl Environ Microbiol, 2001, 67: 2883-2894

[10] Klarzynaski O, Plesse B, Joubert J M, Yvin J C, Kopp M, Kloareg B, Fritig B. Linear β-1,3-glucanase are elicitors of defense responses in tobacco. Plant Physiol, 2000, 124: 1027-1037

[11] Alosi M C, Melroy D L, Park R B. The regulation of gelation of phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase. Plant Physiol, 1988, 86: 1089-1094

[12] Walz C, Juenger M, Schad M, Kehr J. Evidence for the presence and activity of a complete antioxidant defence system in mature sieve tubes. Plant J, 2002, 31: 189-197

[13] Machida Y, Nishihama R, Kitakura S. Progress in studies of plant homologs of mitogen-activated protein (MAP) kinase and potential upstream component in kinase cascades. Crit Rev Plant Sci, 1997, 16: 481-496

[14] Frye C A, Tang D, Innes R W. Negative regulation of defense responses in plants by a conserved MAPKK kinase. Proc Natl Acad Sci USA, 2001, 98: 373-378

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Expression Profile of Landrace Hongyoumai Infected by Blumeria graminis f.sp.tritici Using Gene Microarray

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