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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 855-861.doi: 10.3724/SP.J.1006.2013.00855


Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Xiaoyan 9323

TANG Ming-Shuang,MA Dong-Fang,WANG Hai-Ge,YIN Jun-Liang,HOU Lu,YAO Qiang,JING Jin-Xue*   

  1. State Key Laboratory of Crop Stress Biology for Arid Areas / College of Plant Protection, Northwest A&F University, Yangling 712100, China
  • Received:2012-08-27 Revised:2012-12-15 Online:2013-05-12 Published:2013-02-19
  • Contact: 井金学, E-mail: jingjinxue@163.com, Tel: 029-87092434


Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Xiaoyan 9323, the homology material of Xiaoyan 6, has early maturity, strong resistance, wide adaptability, higher resistance to stripe rust, excellent biological characteristics. To identify the resistance gene(s), we crossed Xiaoyan 9323 with susceptible genotype Mingxian 169, and tested F1, F2, F3, and BC1 progenies with Chinese race CYR32 at seedling stage in greenhouse. Then, SSR techniques were used to screen molecular markers linked to the gene conferring resistance to CYR32 in Xiaoyan 9323. The genetic analysis results showed that Xiaoyan 9323 displayed resistance to epidemic yellow rust races in China when tested with five races ofstripe rust and one recessive gene conferring resistance to races CYR32. The mapping population of F2 generation was inoculated with CYR32. Six SSR markers, Xwmc807, Xbarc3, Xwmc684, Xwmc201, Xwmc553,and Xwmc179 were linked to the resistance gene, which were all located on chromosome arm 6AL. The closest flanking makers were Xwmc201 and Xwmc553 with genetic distances of 2.6 cM and 3.7 cM, respectively. According to SSR markers’ chromosome location, the gene was located at chromosome 6AL. Based on chromosomal location, reactions to various pathotypes andpedigree analysis, the gene may be a new one different from the known stripe rust resistance genes, designated as YrXY9323 temporarily. Two flanking SSR markers, Xwmc201 and Xwmc553, were used to test 42 wheat cultivars from Huang-Huai River Valleys wheat region. The results showed that 19% cultivars had the same polymorphic bands as YrXY9323, indicating that the polymorphic SSR markers can be useful in wheat molecular marker selection and breeding.

Key words: Xiaoyan 9323, Puccinia striiformis f. sp. tritici, Recessive resistance gene, Molecular mapping

[1]Li Z-Q(李振岐), Zeng S-M(曾士迈). China Wheat Rust (中国小麦锈病). Beijing: China Agricultural Press, 2002. pp 41–50, 164–173 (in Chinese)

[2]Line R F. Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol, 2002, 40: 75–118

[3]Li Z-Q(李振岐), Zeng S-M(曾士迈). China Wheat Rust (中国小麦锈病). Beijing: China Agriculture Press, 2002. pp 325–329 (in Chinese)

[4]Wan A-M(万安民), Niu Y-C(牛永春), Wu L-R(吴立人), Yuan W-H(袁文焕), Li G-B(李高宝), Jia Q-Z(贾秋珍), Jin S-L(金社林), Yang J-X(杨家秀), Li X-F(李艳芳), Bi Y-Q(毕云清). Physiology specialization of stripe rust of wheat in China during 1991–1996. Acta Phytopathol Sin (植物病理学报), 1999, 29(1): 15–20 (in Chinese with English abstract)

[5]Wan A-M(万安民), Wu L-R(吴立人), Jia Q-Z(贾秋珍), Jin S-L(金社林), Li G-B(李高宝), Wang B-T(王保通), Yao G(姚革), Yang J-X(杨家秀), Yuan Z-Y(原宗英), Bi Y-Q(毕云青). Pathogenic changes of stripe rust fungus of wheat in China during 1997-2001. Acta Phytopathol Sin (植物病理学报), 2003, 33(3): 261–266 (in Chinese with English abstract)

[6]Yang Z-M(杨作民), Xie C-J(解超杰), Sun Q-X(孙其信). Situation of the source of stripe rust resistance of wheat in the post CY32 era in China. Acta Agron Sin (作物学报), 2003, 29(2): 161–168 (in Chinese with English abstract)

[7]Yang Z-M(杨作民), Tang B-R(唐伯让), Shen K-Q(沈克全), Xia X-C(夏先春). A strategic problem in wheat resistance breeding building and utilization of sources of second line resistance against rusts and mildew in China. Acta Agron Sin (作物学报), 1994, 20(4): 385–394 (in Chinese with English abstract)

[8]Lü W-D(吕伟东), Xu P-B(徐鹏彬), Pu X(蒲训). Summary of the situation for applying genetic resources from Elytrigia in Triticum aestivum breeding. Acta Pratacult Sin (草业学报), 2007, 16(6): 136–140 (in Chinese with English abstract)

[9]Ma J-X(马渐新), Zhou R-H(周荣华), Dong Y-C(董玉琛), Jia J-Z(贾继增). Molecular marker location of yellow rust resistance gene derived from Elytrigia elongate (Host) Nevski. Chin Sci Bull (科学通报), 1999, 44(1): 65–69 (in Chinese with English abstract)

[10]Qin L(秦琳), Li S(李杉), Cui K-R(崔凯荣), Wang Y-F(王亚馥), Ni J-F(倪建福), Zhou W-L(周文麟). RAPD Verification of rust resistance wheat variants. Acta Bot Boreal-Occident Sin (西北植物学报), 1997, 17(6): 22–26 (in Chinese with English abstract)

[11]Liu D-C(刘登才), Zheng Y-L(郑有良), Wang Z-R(王志容), Hou Y-C(侯永翠), Lan X-J(兰秀锦), Wei Y-M(魏育明). Distribution of chromosomes in diploid Lophopyrum elongatum (Host) A. Löve that influences resistance to head scab of common wheat. J Sichuan Agric Univ (四川农业大学学报), 2001, 19(2): 200–205 (in Chinese with English abstract)

[12]Hou Y-C(侯永翠), Zheng Y-L(郑有良), Liu D-C(刘登才), Lan X-J(兰秀锦). Resistance to powdery mildew of the Ee chromosomes of Lophopyrum elongatum (Host) A. Löve in Chinese Spring background. J Sichuan Agric Univ (四川农业大学学报), 2000, 18(1): 25–27 (in Chinese with English abstract)

[13]Liu X-K(刘孝坤). A preliminary study on the inheritance of resistance to stripe rust in wheat. Acta Phytophyl Sin (植物保护学报), 1988, 15(1): 33–39 (in Chinese)

[14]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69–76

[15]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832

[16]Röder M S, Korzun V, Wendehake K, Plaschke J. A microsatellite map of wheat. Genetics, 1998, 149: 2007–2023

[17]Pestsovca E, Ganal M W, Röder M S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 2000, 43: 689–697

[18]Song Q J, Shi J R, Sigh S, Fickus E W. Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet, 2005, 110: 550–560

[19]Liu R-H(刘仁虎), Meng J-L(孟金陵). Map Draw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (遗传), 2003, 25(3): 317–321 (in Chinese with English abstract)

[20]Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105–1114

[21]Marais G F, McCallum B, Marais A S. Leaf rust and stripe rust resistance genes derived from Aegilops sharonensis. Euphytica, 2006, 149: 373–380

[22]Jing J-X(井金学), Xu Z-B(徐智斌), Wang D-B(王殿波), Wang M-N(王美南), Yao Q-Y(姚秋燕), Shang H-S(商鸿生), Li Z-Q(李振岐). Genetic analysis of gene conferring resistance tostripe rust in Xiaoyan 6. Sci Agric Sin (中国农业科学), 2007, 40(3): 499–504 (in Chinese with English abstract)

[23]Chen X-H(陈新宏), Zhao J-X(赵继新), Wu J(武军), Liu S-H(刘淑会). The breeding of new super yielding and developing of matching grown of Xiaoyan 216. Bull Agric Sci Technol (农业科技通讯), 2008, (7): 141–143 (in Chinese with English abstract)

[24]Shang H-S(商鸿生). High temperature resistance of wheat to stripe rust. Sci Agric Sin (中国农业科学), 1988, 31(4): 46–50 (in Chinese with English abstract)

[25]Li-Q(李强), He M-M(贺苗苗), Dong H-L(董海丽), Yao Q(姚强), Jing J-X(井金学), Wang B-T(王宝通). Genetic analysis and molecular mapping for stripe rust resistance gene(s) in wheat cultivar Guinong 22. Acta Phytopathol Sin (植物病理学报), 2011, 41(5): 495–501 (in Chinese with English abstract)

[26]Zhou X L, Wang W L, Wang L L, Hou D Y, Jing J X, Wang Y, Xu Z Q, Yao Q, Yin J L, Ma D F. Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54. Theor Appl Genet, 2011, 123: 431–438

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