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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1273-1281.doi: 10.3724/SP.J.1006.2016.01273

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Global Identification and Comparison of MicroRNA in Wild and Cultivated Adzuki Bean

MA Yan-Ming1,CHEN Chun-Hai2,YANG Kai1,LI Yi-Song1,ZHAO Bo1,LI Jiang2,LI Yong-Qiang1,WAN Ping1,*   

  1. 1 Beijing University of Agriculture /Beijing Key Laboratory of New Technology in Agricultural Application, Beijing 102206, China; 2BGI-Shenzhen, Shenzhen 518083, China
  • Received:2016-01-27 Revised:2016-05-04 Online:2016-09-12 Published:2016-05-09
  • Contact: 万平, E-mail: pingwan3@163.com, Tel: 010-80799134 E-mail:574088090@qq.com
  • Supported by:

    This study was supported byNational Natural Science Foundation of China (31371694)

Abstract:

MicroRNAs (miRNAs) are a class of small regulatory RNAs with thefunction as crucial regulators that repress the expression of their target genes at the transcriptional or post-transcriptional level. Global identification of miRNA is important for the functional study. Adzuki bean (Vigna angularis) genome sequencing has beenfinished recently, but lack of a systematical analysis of miRNA. In this study, we performed deep miRNA sequencing in wild (CW108) and cultivated (JN6) adzuki beans. Our investigation not only identified the miRNAs that shared by these two species, but also found miRNAs that specifically existed in individual accession. Through comparison of the miRNA expression and their target gene predictions between the wild and cultivated adzuki beans, we found that the miRNA was diverged between them, which may be associated with plant-pathogen interaction pathway. Our study provided a valuable resource for functional analysis in adzuki bean.

Key words: miRNA, Wild adzuki bean(Vignaangularis), Cultivated adzuki bean(Vignaangularisvar. nipponensis)

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