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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 849-854.doi: 10.3724/SP.J.1006.2017.00849


Identification of Promoter GhFLA1 Preferentially Expressed during Cotton fiber Elongation

HU Hai-Yan,LIU Di-Qiu,LI Yun-Jing,LI Yang,TU Li-Li*   

  1. National Key Laboratory of Crop Genetic Improvement / Huazhong Agricultural University, Wuhan 430070, China
  • Received:2016-08-08 Revised:2017-01-21 Online:2017-06-12 Published:2017-02-17
  • Contact: 涂礼莉, E-mail: lilitu@mail.hzau.edu.cn, Tel: 027-87283955
  • Supported by:

    This work supported by the National Natural Science Foundation of China (31071465).


Cotton (Gossypium hirsutum L.) fiber is a perfect model cell for studying cell initiation and growth, so it is critical to find new promoters specifically or preferentially expressed in fiber for functional genomics of fiber and improvement of cotton fiber yield and quality. We found a gene GhFLA1 with very high expression level in fiber elongation stage and cloned its promoter (pGhFLA1). pGhFLA1::GUS was transferred into cotton YZ1 (Gossypium hirsutum) and tobacco (Nicotiana tabacum) to identify its expression pattern in different plant tissues. GUS activity in transgenic plant was obviously detected in fiber from 0 DPA (days post anthesis) to 20 DPA, also in stamens, pistils, sepal, radicle, cotyledon and root, but not in petal, leaf and bract. Quantitative analysis of GUS activity showed that GUS expression level driven by pGhFLA1 was highest in 20 DPA fiber and 22-fold higher than that in CaMV 35S. In transgenic tobacco, GUS activity was detected in leaf and trichome and reproductive organs like ovary, stigma, anther, bract, petal and seed. In general, the promoter pGhFLA1 fusion with GUS highly expresses in cotton fiber and tobacco trichome, which could be used in fiber functional genomics research and improvement of cotton fiber quality.

Key words: Cotton (Gossypium hirsutum L.), GhFLA1, Promoter, Fiber

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