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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 855-861.doi: 10.3724/SP.J.1006.2017.00855

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Breeding New Peanut Line with High Oleic Acid Content Using Backcross Method

YU Ming-Yang1,SUN Ming-Ming1,GUO Yue1,JIANG Ping-Ping1,LEI Yong2,HUANG Bing-Yan3,FENG Su-Ping4,GUO Bao-Zhu5,SUI Jiong-Ming1,WANG Jing-Shan1,QIAO Li-Xian1,*   

  1. 1 Key Lab of Plant Biotechnology in Universities of Shandong Province / College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China; 2 Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China; 3 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 4 College of Life Sciences and ecology, Hainan Tropical Ocean University, Sanya 572022, China; 5 USDA-Agricultural Research Service, Tifton, GA, 31793-074, USA
  • Received:2016-04-06 Revised:2017-01-21 Online:2017-06-12 Published:2017-02-17
  • Contact: Qiao Lixian，E-mail: lxqiao73@163.com, Tel: 0532-86080640 E-mail:ymy960571691@163.com
  • Supported by:

    This program was financially supported by the National Natural Science Foundation of China (31471524, 31571705), the Mars-China High Oleic Acid Peanut Breeding Project, and the Shandong Province Science and Technology Development Plan Project (2014GNC110002).

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Abstract:

F1 hybrids and BC1F1–BC4F1 backcross generation were obtained by cross and backcross with peanut variety HY22 as female parent (recurrent parent) and Kainong176 with high oleic acid content as the donor parent. The contents of oleic acid and linoleic acid of F1 and BC1F1 to BC4F1 were determined by Near infrared spectrometer. The seeds with oleic acid content higher than 60% were selected and their partial cotyledon was cut off for DNA extraction. The loci FAD2a and FAD2b in these seeds were detected by sequencing absorption peaks of PCR products with F0.7/R3 as primers, and those seeds with both FAD2a and FAD2b were used as the male parent in following backcross. The incision in these seeds whose cotyledon was cut off was sealed by paraffin oil, and these seeds were soaked in water for sprouting at 40°C. Those seeds that did not germinate within 12 hours were soaked in 100 mg L–1 ethephon solution for four hours, then immersed into water till 24 hours at 40°C by which 98% seeds could germinate. Four to five times of backcross every two years were finished in spring in Qingdao, 1–2 times in autumn and winter in Sanya, by which the breeding process might be accelerated. The backcrossing work began in the spring of 2013 and BC4F2 seeds were sowed in Qingdao in the spring of 2016. The young leaves were used for genotyping and these plants with aabb genotype and similar agronomic characters with Huayu 22 were selected and harvested, whose oleic content was then confirmed by NIR. Twenty single plants with similar characters of Huayu 22 whose oleic content was higher than 70% and oleic/linoleic ratio was higher than 7.0 were regarded as a new improved HY22 strain with high oleic acid content.

Key words: Peanut (Arachis hypogaea L.), High oleic acid content, Backcross, Sequencing, F0.7/R3

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