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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (02): 238-248.doi: 10.3724/SP.J.1006.2013.00238

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Recombination in Wheat Using SSR Markers

LI Xiao-Jun,FENG Su-Wei,LI Gan,DONG Na,CHEN Xiang-Dong,SONG Jie,RU Zhen-Gang*   

  1. Center of Wheat Breeding, Henan Institute of Science and Technology / Key Discipline Open Laboratory on Crop Molecular Breeding of Henan Institute, Xinxiang 453003, China
  • Received:2012-07-30 Revised:2012-10-09 Online:2013-02-12 Published:2012-12-11
  • Contact: 茹振钢, E-mail: rzgh58@sohu.com

Abstract:

To understand the characteristics of inheritance and recombination of parental chromosome fragments in wheat progenies, we screened the genomes of 23 genotypes derived from Zhoumai 18 and Bainong AK58 with 340 SSR markers covering the whole wheat genome, together with the parents. The average recombination frequency in cultivars from single-cross was 12.3, which was smaller than that in cultivars from single backcross (13.9). Recombination mostly occurred on chromosomes 4A, 5A, 7A, 1B, 3B, 4B, 7B, 1D, 2D, 3D, 5D, 6D, and 7D. The distal and central chromosomal regions had similar frequencies of recombination which were 6.1, and 6.0, respectively. Some chromosomal regions were hot in recombination, such as marker intervals gwm358–wmc357 on chromosome 5D, cfd49–barc196 on chromosome 6D, wmc158–barc23 on chromosome 7A, and gwm274–gwm146 on chromosome 7B, with 35, 19, 15, and 14 recombination events, respectively. The analysis for inheritance of large linkage blocks indicated that large chromosome fragments inherited from one parent varied from 14 to 29 in each derivative, with 2–8 consecutive and informative SSR loci in a fragment. These large fragments were mainly distributed on chromosomes 4A, 5A, 5B, 5D, and 7D, which might harbor genes controlling important agronomic traits.

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