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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (06): 979-991.doi: 10.3724/SP.J.1006.2013.00979

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences

LIU Yan,CHENG Xu-Zhen*,WANG Li-Xia,WANG Su-Hua,BAI Peng   

  1. Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences
  • Received:2012-11-16 Revised:2013-01-15 Online:2013-06-12 Published:2013-03-20
  • Contact: 程须珍, E-mail: chengxz@caas.net.cn, Tel: 010-62180535

Abstract:

China has abundant germplasm resources of subgenus Ceratotropis, but the related study of species within this subgenus is much lagged. In order to investigate genetic relationship and evolution process among different species, 110 entries of Ceratotropis were analyzed by using intron rpl16, rps16 and intergenic regions psbA-trnH, rpoB-trnC, and trnL-trnF of chloroplast genome. The results revealed that parsim-info site percentage was from 3.63% to 24.28%, with the highest for rps16 and the lowest for rpoB-trnC. A higher level conservation of sequence was observed within species than inter-species. Kimura-2-parameter distance was between 0 and 0.057 calculated based on five chloroplast assembling sequences. V. minima complex (including V. minima, V. riukinensis and V. nakashime) and V. subramaniana had the longest distance. Phylogenetic trees constructed by neighbor-joining (NJ) and maximum parsimony (MP) methods in MEGA 5.1 and PAUP 4.0 revealed that eighteen species and subspecies were divided into two branches. The group I contained nine species and subspecies grouped into three sections complex, including Angulares, Ceratotropis and Aconitifoliae. The group II contained nine species and subspecies which completely belonged to section Angulares. Partition homogeneity test of phylogenetic trees of the five sequences showed that their topology was highly consistent (P=0.87). The present study provides the important information for clarifying genetic relationship Ceratotropis. among different species within

Key words: Subgenus Ceratotropis, Chloroplas DNA, Sequence difference, Genetic evolution

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