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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (07): 1164-1173.doi: 10.3724/SP.J.1006.2014.01164

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

In silico Expression Profile of Maize Genes in Response to Osmotic Stress

GE Shu-Juan1,**, SUN Ai-Qing2,**, LIU Peng2, ZHANG Jie-Dao1,*,DONG Shu-Ting2,*   

  1. 1 State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China;
    2 College of Agriculture, Shandong Agricultural University, Tai’an 271018, China
  • Received:2014-01-21 Online:2014-07-12 Published:2014-07-12

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Abstract:

In this study, a germplasm POB21 was used to analyze leaf cDNA library of maize treated with 15% PEG by in silico expression profile. The results indicated the POB21 genome shared significantly high similarity with reference genome. The gene expression in maize transcriptome presented strong nonhomogeneity and partial redundancy. A total of 1097 differentially expressed genes (DEGs) were screened out, of which 795 DEGs were up-regulated and 302 DEGs were down-regulated. GO enrichment analysis of these DEGs showed that three cellular components and three molecular functions of glycosyl transferase activity were enriched. The KEGG pathway analysis showed these DEGs were involved in metabolisms of carbohydrate, protein, nucleic acid, lipid, secondary metabolites, hormones and energy. The DEGs involving in proline metabolism indicated glutamate pathway is the predominant accumulation way of proline under osmotic stress in maize. The result lay a foundation for further study of molecular mechanism in response to osmotic response and functional genes screening of maize.

Key words: Maize, Osmotic stress, Gene expression profile, Differentially expressed genes

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