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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (4): 628-634.doi: 10.3724/SP.J.1006.2019.84086

• RESEARCH NOTES • Previous Articles     Next Articles

Allohexaploid synthesis and its characteristic identification between cotton species Gossypium hirsutum and G. sturtianum

SHEN Zhuang-Zhuang,LI Yu-Ying,RONG Er-Hua,WU Yu-Xiang()   

  1. College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
  • Received:2018-06-22 Accepted:2019-01-12 Online:2019-04-12 Published:2019-02-03
  • Contact: Yu-Xiang WU E-mail:yuxiangwu2009@hotmail.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31171599);National Foundation Fostering Matching Project of Shanxi Agricultural University(2017GPY04);Key Research and Development Program Projects in Shanxi Province.(201803D221018-7)

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Abstract:

Gossypium hirsutum is an important cultivated cotton species and G. sturtianum is a wild cotton species with many excellent characteristics. In this study, we obtained fertile offsprings by interspecific distant hybridization and chromosome doubling between G. hirsutum and G. sturtianum. Through flow cytometry and meiosis observation of pollen mother cells, two heterohexaploid plants with complete chromosomes were selected. Further, the characteristic identification of their leaves, flowers and seeds was carried out, showing that the leaf shape of hexaploid was between their parents with increased leaf area, and the number of chloroplasts in guard cells obviously more than that of their parents and triploid. The flowers were significantly different between distant hybrids and their parents, and the pollen grain diameter of hexaploid was larger than that of parents and triploid. The fiber color of hexaploid was white, and the fiber length was shorter than that of G. hirsutum, and the traits of delayed seed gland development coming from G. sturtianum were expressed in the allohexaploid hybrids. These results of synthesized new fertile allohexaploid germplasms between G. hirsutum and G. sturtianum provide new valuable materials for the study of cotton genetics and breeding.

Key words: G. sturtianum, distant hybrid, allohexaploid, delayed seed gland development

Table 1

Flow cytometry results for ploidy level in different generations"

P2 F1 P1 M1-1 M1-2 M1-3 M1-4 M1-5 M1-6
X-mean 98.81 122.89 140.44 153.02 184.70 190.86 239.88 239.92 254.28

Fig. 1

Meiosis performance of pollen mother cells A: metaphase II of P1; B: anaphase II of P1; C: tetrad of P1; D: anaphase I of F1; E: anaphase I of F1; F: abnormal tetrad of F1; G: diakinesis of M1; H: metaphase II of M1; I: tetrad of M1."

Fig. 2

Leaf morphology comparison among different generations"

Table 2

Difference significance analysis of Leaf traits among different generations"

材料
Material		 倍性
Ploidy		 叶面积
Leaf area (cm2)		 叶形指数(叶长/叶宽)
Leaf index (leaf length/leaf width)		 叶绿体数
Chloroplast number
P1 4 39.47 ± 12.32 bcBC 0.77 ± 0.09 aA 13.57 ± 1.59 bB
P2 2 16.38 ± 3.20 aA 1.07 ± 0.06 dC 12.92 ± 1.55 bB
F1 3 32.70 ± 5.82 bB 0.93 ± 0.06 cB 11.64 ± 1.77 aA
M1 6 43.97 ± 17.11 cC 0.82 ± 0.06 bA 19.95 ± 2.97 cC

Fig. 3

Trends of leaf area, leaf index and chloroplast number in different ploidies"

Fig. 4

Phenotype comparison of flowers among different generations (bar: 20 mm)"

Fig. 5

Frequency histogram of pollen grain diameter among different generations"

Fig. 6

Comparison of seed gland traits among different generations"

Fig. 7

Comparison of cotyledon gland traits among different generations"

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