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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 984-989.doi: 10.3724/SP.J.1006.2016.00984


Detection of the Molecular Marker and Chromosomal Segment linked to Unreduced Gamete Gene in Common Wheat

KOU Chun-Lan,ZHAO Lai-Bin,LIU Meng,HAO Ming,NING Shun-Zong,YUAN Zhong-Wei,LIU Deng-Cai,ZHANG Lian-Quan*   

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China
  • Received:2015-11-04 Revised:2016-03-14 Online:2016-07-12 Published:2016-03-08
  • Contact: 张连全, E-mail: zhanglianquan1977@126.com, Tel: 028-82650313 E-mail:510531588@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271723 and 31201210), Sichuan Provincial Youth Fund (2011JQ0016), and the Scienti?c Research Foundation of the Education Department of Sichuan Province (14ZA0012).


Hexaploid common wheat (Triticum aestivum L., AABBDD, 2n=42) arose fromspontaneous chromosome doubling of the hybrid between T. turgidum and Aegilops tauschii Cosson. The process of chromosomes doubling is mainly determined by unreduced gametes (UG)genes in T. turgidum. The genetic effects on the UG production may vary among T. turgidum lines. In this study, a SSR marker close to the UG geneQTug.sau-3B(Xgpw1146) and high throughput DArTseq genotyping technique were used to screen the UG gene in common wheat lines transferred from T. turgidum via synthetic hexaploid wheat (SHW) as a bridge. Out of the analyzed 105 SHW-derived elite lines, 17 had the Xgpw1146allele from T. turgidum, indicating that the UG gene was probably transferred into these wheat lines. According to the DArTseq genotyping data on 88 lines derived from the synthetic hexaploid wheat SHW-L1, all these lines with the T. turgidumXgpw1146allele contained a chromosomal segment of SHW-L1,probably covering the Xgpw1146 locus. This indicatesthat the adjacent region of the UG gene as a chromosomal segment was transferred into wheat lines. These SHW-derived lines have important application potential on wheat doubled haploid breeding.

Key words: Allohexaploid, Synthetic wheat, Unreduced gamete

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