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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 721-727.doi: 10.3724/SP.J.1006.2010.00721


Molecular Tagging of a Stripe Rust Resistance Gene in a Triticum durumAegilops squarrosa Synthetic Wheat CI191

REN Qiang1,2,LIU Hui-Juan1,**,CHEN Yang1,XU Shi-Chang3,HE Ming-Zhao1,XIN Zhi-Yong1,ZHANG Zeng-Yan1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:2009-11-24 Revised:2010-02-07 Online:2010-05-12 Published:2010-03-15
  • Contact: ZHANG Zheng-Yan,E-mail: zhangzy@mail.caas.net.cn; Tel: 010-82108781


Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most serious fungal diseases in wheat worldwide. To diversify the resistance resources, a resistance synthetic wheat CI191 (CPI/GEDIZ/3/GOO//JO69/CRA/ 4/AE.SQ629) has been mined from 94 accessions of Triticum durum-Aegilops squarrosa synthetic wheat introduced from CYMMT. CI191 show highly resistant to six Pst races prevailing in Chinese, such as CY28, CY29, CY30, CY31, CY32, and CY-Su11-4. Based on the responses of CI191 nd 24 wheat materials possessing known stripe rust resistance genes to 22 Pst races, we postulated CI191 was different from all the known resistant genes. According to analysis of inherited mode, the resistance gene in CI191 was controlled by a single dominate gene, tentatively designated YrC191. Using the cross between CI191 and Mingxian 169 (a sensitive wheat cultivar to Pst races), the F2 segregation population and the bulked segregant pools were established to screen the the simple sequence repeat (SSR) markers linked to YrC191. Seven SSR markers were found to be linked with YrC191 in the alignment of Xwmc419-YrC191/ Xbarc240-Xbarc8-Xcfd65/Xbarc187/Xgwm18/Xgwm11. YrC191 was cosegrated withthe marker Xbarc240, flanked by the closest linked markers Xbarc8 and Xwmc419 with the relative genetic distances of 1.6 cM and 3.1 cM, respectively. Using Chinese Spring nullisomic-tetrasomics and ditelosomic lines of homoeologous group 1, Xcfd65, Xgwm18, Xgwm11, Xbarc187, and Xwmc419 markers were physically mapped on the chromosome arm 1BS, and YrC191 was also located on 1BS. Four SSR markers linked tightly with YrC191 and 1 sequence tagged site (STS) marker for Yr26 could discriminate YrC191 from the other resistant genes on 1BS, such as Yr24, Yr26, Yr10, Yr15, YrCH42, and YrC142. The resusltes suggested that YrC191 may be a novel resistance gene to Pst.

Key words: Triticum durum/Aegilops tauschii synthetic wheat, stripe rust, resistance gene, gene postulation, SSR marker

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