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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (12): 2314-2323.doi: 10.3724/SP.J.1006.2021.04266


Genome-wide identification of peanut resistance genes and their response to Ralstonia solanacearum infection

ZHANG Huan**(), LUO Huai-Yong**(), LI Wei-Tao, GUO Jian-Bin, CHEN Wei-Gang, ZHOU Xiao-Jing, HUANG Li, LIU Nian, YAN Li-Ying, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang*()   

  1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2020-12-08 Accepted:2021-04-14 Online:2021-12-12 Published:2021-06-15
  • Contact: JIANG Hui-Fang E-mail:18198335427@163.com;huaiyongluo@caas.cn;peanutlab@oilcrops.cn
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(31761143005);National Natural Science Foundation of China(31971903);Central Public-interest Scientific Institution Basal Research Fund(1610172019008)


Peanut is one of the main oil crops, which is harmed by many pathogenic microorganisms during growth and development period. Breeding and selection of disease-resistant varieties is one of the most economical and effective ways to control disease, and disease resistance genes are important genes for plant resistance to pathogenic microorganisms. Here, the whole genome-wide identification of peanut disease resistance genes was carried out for the first time. A total of 4156 candidate disease resistance genes were identified. Among them, 536, 490, 232, 182, and 149 genes were RLK, RLP, NL, CNL, and TNL, respectively. The distribution of disease resistance genes was uneven on chromosomes, and most of them were concentrated on chromosome B02. Transcriptome profiling revealed that 111 genes were specifically expressed in resistant materials, 104 genes were specifically expressed in susceptible materials, 2216 genes were expressed in both resistant and susceptible materials, while 1725 genes were not expressed in both resistant and susceptible materials. Two kinds of differentiate expressed R genes were identified, including five genes in the first group responded to the infection of Ralstonia solanacearum at specific time and 65 genes in the second group which exhibited higher expressions in resistant cultivar than susceptible cultivar. A candidate gene Arahy.5D95TJ was successfully validated by qRT-PCR. In this study, the identification and analysis of peanut disease resistance genes provides the important reference for further research of their functions and molecular breeding of peanut disease resistance.

Key words: Arachis hypogaea, disease resistance gene, bacterial wilt, transcriptome profiling

Table 1

Number and type of R genes in peanut"

Domain contained
Gene number
Proportion in R gene (%)
RLK TM, LRR, Kinase 536 12.90
RLP TM, LRR 490 11.79
CNL CC, TM, NBS, LRR 182 4.38
TNL TM, TIR, NBS, LRR 149 3.59
KIN TM, Kinase 1714 41.24
NL TM, NBS, LRR 232 5.58
CK CC, TM, Kinase 221 5.32
N TM, NBS 146 3.51
CN CC, TM, NBS 136 3.27
CTNL CC, TM, TIR, NBS, LRR 77 1.85
L LRR 76 1.83
T TM, TIR 65 1.57
TN TM, TIR, NBS 61 1.47
CL CC, TM, LRR 28 0.67
CLK CC, TM, LRR, Kinase 20 0.48
CNT CC, TM, NBS, TIR 15 0.36
CT CC, TM, TIR 6 0.14
TRAN TM 2 0.05
总计Total 4156

Fig. 1

Distribution of R genes on peanut chromosomes Expressed in both resistant and susceptible materials are marked by green; only expressed in resistant materials are marked by red; only expressed in susceptible materials are marked by purple; not expressed in resistant and susceptible materials are marked by black."

Fig. 2

Distribution range of FPKM values of R genes in peanut RC: control of resistant material; RT: treatment of resistant material; SC: control of susceptible material; ST: treatment of susceptible material."

Fig. 3

Two classes of differentially expressed R genes infected by Ralstonia solanacearum in peanut RC: control of resistant material; RT: treatment of resistant material; SC: control of susceptible material; ST: treatment of susceptible material. RT12, RT 24, RT 48, RT 72, and RT 96 represented the resistant materials collected at 12, 24, 48, 72, and 96 hours post inoculated with Ralstonia solanacearum (treatment group); RC12, RC24, RC48, RC72, and RC 96 represented the resistant materials collected at 12, 24, 48, 72, and 96 hours post inoculated with water (control group); ST12, ST24, ST48, ST72, and ST 96 represented the susceptible materials collected at 12, 24, 48, 72, and 96 hours post inoculated with Ralstonia solanacearum (treatment group); SC12, SC24, SC48, SC72, and SC96 represented the susceptible materials collected at 12, 24, 48, 72, and 96 hours post inoculated with water (control group). FPKM: fragments per kilobase per million bases."

Fig. 4

Differentially expressed R genes in the confidential interval of the major QTL for resistance to bactrierl wilt Abbreviations are the same as those given in Fig. 3."

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JIANG Hui-Fang ;LIAO Bo-Shou;REN Xiao-Ping;LEI Yong;FU Ting-Dong;Mace E ;Crouch J H

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