甘蓝型春油菜早花位点加密及位点聚合创建优异早花资源

doi:10.3724/SP.J.1006.2020.04012

Abstract

Abstract:

cqDTFA7a and cqDTFC8, two major effects of early flowering QTL, were identified in the NNDH population of spring Brassica napus, and closely linked markers SSR G1803, InDel IA7-4, and SSR S035 with cqDTFC8 developed in previous studies. In this study, a BC₂F₂ population for early flowering QTL locus cqDTFC8 was constructed, and a closely linked SNP marker was further developed. The early flowering genotypes of one natural resources contained 93 spring B. napus varieties were identified used four closely linked markers with two loci, and selected 3164 and 2216 resources with cqDTFA7a site, 3484 and 2857 resources with cqDTFC8 site. Two site resources were aggregated by site polymerization through reciprocal hybridization. The polymerized DH system was rapidly obtained by microspore culture and maker assisted selection. A hybrid combination was created between polymeric line with good traits and early flowers and the Polima CMS, and the utilization value of the polymeric line was further analyzed by the production test at multiple environments for two consecutive years. cqDTFC8 encryption results showed that this site was located in the SNP11 and SNP12 interval, and separated from SNP11 altogether. The identification results of early flowering genotypes of natural resources showed that there were 50 individuals containing cqDTFA7a locus, with an average initial flowering period of 58.1 days; 16 single plants containing cqDTFC8 locus, with an average flowering period of 58.3 days; and 16 single plants containing two loci, with an average flowering period of 55.2 days, indicating the more early flowering sites containing, the earlier flowering. The results of polymerization showed that the flowering time of the polymerized lines of cqDTFC8 and cqDTFA7a was 2-3 days earlier than that of the single locus parents, among which the polymerized DH18 from 3164 of cqDTFA7a and 3484 of cqDTFC8 was 3 days earlier than that of the parents, and the yield-related traits were better than those of other lines. The combination of DH18 and the Polima CMS 025A was further utilized, and the combination was named TZG18. Yield results of two years and nine environments showed that yield of TZG18 was above 17.5% higher than the Haoyou 11, a local B. rapa variety on the Qinghai-Tibet plateau. Those results indicated that the early flowering site polymeric lines had an obvious advantage over the single locus lines in flowering time, and had an effect on the increasement of rapeseed yield. This study is a preliminary exploration of MAS breeding for early flowering traits of Brassica napus, providing materials support for replacing B.rapa varieties using early maturity Brassica napus varieties in spring rapeseed region, and approaches for gene polymerization breeding technology.

Key words: spring Brassica napus, early flowering locus, gene pyramiding, early flowering resources

LIU Hai-Dong, PAN Yun-Long, DU De-Zhi. Locus encryption for early flowering and QTL polymerization to create excellent early flowering resources of spring Brassica napus L.[J].Acta Agronomica Sinica, 2020, 46(11): 1711-1721.

Figures/Tables 6

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References 35

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引物 Primer	位置 Position (bp)	突变、插入/缺失 Mutate, insert/miss	上游引物序列 Forward primer (5°-3°)	下游引物序列 Reverse primer (5°-3°)
SNP6	1636463	G/A	CCAGACCCAACATTACCC	TCCGGTGTTCTGCTTATT
SNP11	2777872	G/A	GAAAGCTGCTGGGTTTCT	TATTATGCCATGTCATCTAC
SNP12	2833887	A/G	CTCGGACGAACAGAACCC	TCAGCTCATGGAGGAACA
SNP14	3147552	T/C	TTGAAGTCGTGCGTCGGT	AGCAGAGTAGAGGAAGCTGGTA
SNP20	3674261	T/C	GAGACAAATCTCAGCAGT	CAAGATGACGAATCCAAT
SNP21	3706251	T/C	GTTTATGTCTGACCTTGTT	GTGGAACTTGATGGATTT
InDel 53	5405790	CA/C	ATAGACGTAGAGGCTGAGCA	AATCTCGTCACCATCAAGAG
InDel 55	5409462	GAATAACATAAAACTT/G	GGTGTTCATCTCTGAAGACAA	TTGTGATTTGTGAATTGATGA
InDel 60	5527563	GTCGC/G	CATGCTTCATCTCTTCAACA	GTCAACCAGCGAGTAGAAAC

基因型Genotype				资源数 Number of resources	初花期First flowering period (d)
cqDTFA7a		cqDTFC8		资源数 Number of resources	变幅 Range	平均值±标准差 Mean±SD	P值 P-value
G1803	IA7-4	S035	SNP11
AA				56	53.2-62.1	58.7±4.3	0.04^a
BB				37	63.2-77.3	65.7±5.2
	AA			50	54.2-62.3	58.1±3.9	0.04^b
	BB			43	63.5-78.2	65.9±4.8
		AA		21	55.7-61.7	58.6±3.6	0.04^c
		BB		72	62.3-77.7	64.9±4.1
			AA	16	52.4-59.3	58.3±3.7	0.04^d
			BB	77	62.1-79.3	64.4±5.1
AA	AA	AA	AA	16	53.2-57.6	55.2±3.2	0.008^e
BB	BB	BB	BB	30	65.4-79.3	65.0±4.6

材料名称 Material name	位点 Locus	来源 Source	农艺性状Agronomic trait					开花时间 Flowering time (d)
材料名称 Material name	位点 Locus	来源 Source	角果长 Fruit length (cm)	每角粒数 Seed number	千粒重 Thousand seeds weight (g)	全株角果数 Carob number	单株产量 Yield per plant (g)	开花时间 Flowering time (d)
3164	cqDTFA7a		7.8±1.9 bc	29.6±1.0 a	3.8±0.2 cd	293±28.0 bc	16.7±2.4 b	58.3±2.2 abc
2216	cqDTFA7a		7.6±1.2 c	25.0±2.5 bcd	4.2±0.1 bcd	274±40.5 cd	16.5±3.4 b	59.2±2.1 ab
3484	cqDTFC8		8.0±1.0 ab	27.2±3.0 b	4.2±0.3 abc	341±10.1 ab	17.5±3.7 ab	57.6±2.3 abc
2857	cqDTFC8		6.9±1.1 e	26.3±3.0 bc	3.6±0.2 f	271±14.5 cde	14.7±2.6 b	60.0±3.0 a
DH18	cqDTFC8 & cqDTFA7a	3484×3164	8.1±1.1 a	32.0±2.0 a	4.5±0.1 a	354±26.6 a	21.4±2.1 a	54.5±1.2 c
DH2	cqDTFA7a & cqDTFC8	2216×3484	7.0±1.9 e	23.1±5.0 d	4.1±0.1 cd	264±29.5 cde	18.5±2.2 ab	55.6±2.0 bc
DH20	cqDTFA7a & cqDTFC8	3164×3484	7.2±1.1 d	23.6±7.0 cd	3.6±0.2 ef	339±37.0 ab	17.1±2.0 ab	55.8±1.2 bc
DH12	cqDTFA7a & cqDTFC8	2216×2857	7.1±1.6 de	23.4±3.0 cd	4.3±0.1 abc	257±38.5 sdef	16.3±3.2 b	56.2±2.1 abc
DH5	cqDTFA7a & cqDTFC8	3164×2857	6.8±0.9 e	27.6±3.5 b	3.9±0.4 de	216±21.3 f	16.4±2.2 b	56.4±1.2 abc
DH4	cqDTFC8 & cqDTFA7a	3484×2216	6.5±0.4 e	25.2±5.1 bcd	4.1±0.1 bcd	239±17.2 def	15.8±3.1 b	56.8±1.4 abc
DH23	cqDTFC8 & cqDTFA7a	2857×3164	6.8±0.1 e	27.8±3.2 b	4.4±0.2 ab	225±22.5 ef	16.7±4.1 b	57.3±1.3 abc

品种 Variety	产量Yield (kg hm^-2)						平均 Average	比对照增产 Yield increase over control (%)
品种 Variety	门源沙沟梁 Menyuan shagouliang	门源后院 Menyuan backyard	湟源 Huangyuan	同德 Tongde	贵南牧场 Guinan Ranch	海北 Haibei	平均 Average	比对照增产 Yield increase over control (%)
2018
TZG18	620.0±80.0 a	980.0±166.7 a	2233.3±100.0 a	1733.3±133.3 a			1391.7±73.3 a	21.0
浩油11号 Haoyou 11 (CK)	686.7±93.3 a	1113.3±160.0 a	1986.7±140.0 b	813.3±60.0 b			1150.0±53.3 b	0
2019
TZG18		1746.7±160.0 a	2960.0±206.7 a	2500.0±106.7 a	2453.3±140.0 a	2780.0±233.3 a	2488.0±113.3 a	17.5
浩油11号 Haoyou 11 (CK)		1146.7±0.14 b	2393.3±186.7 b	2213.3±80.0 b	2566.7±153.3 a	2266.7±160.0 b	2117.3±93.3 b	0

Locus encryption for early flowering and QTL polymerization to create excellent early flowering resources of spring Brassica napus L.

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