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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (12): 1970-1978.doi: 10.3724/SP.J.1006.2020.04010

• TILLAGE & CULTIVATION?PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Phytoremediation potential of five industrial hemp varieties on five heavy metal polluted soils

Yan-Ping XU1,2(), Ming YANG2, Hong-Yan GUO2, Qing-Hui YANG1,*()   

  1. 1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
    2Industrial Crop Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
  • Received:2020-01-13 Accepted:2020-08-19 Online:2020-09-04 Published:2020-09-25
  • Contact: Qing-Hui YANG E-mail:cyn080328@126.com;yangqinghui@163.com
  • Supported by:
    National Natural Science Foundation of China(31660351);China Agriculture Research System for Bast and Leaf Fiber Crops(CARS-19-E05)

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Abstract:

Uptake and translocation of lead (Pb), zinc (Zn), copper (Cu), cadmium (Cd), and arsenic (As) in industrial hemp genotypes were investigated at seedling and physiological maturity, in order to provide an insight for phytoremediation of mining soils in Yunnan. The experiment was conducted using five main varieties of industrial hemp viz., Yunma (ym)1, ym2, ym3, ym4, and ym5 under mining areas of Yunnan. Results showed that uptake and translocation of Pb, Zn, Cu, Cd, and As in all hemp genotypes were high at the seedling stage. Accumulation of Pb, Zn, Cu, Cd, and As contents in hemp roots were higher at physiological maturity as compared to seedling stage. However, comparing to seedling stage, Pb, As, and Cd contents in stems and leaves were higher at physiological maturity stage. Translocation factor coefficients of hemp stem and leaf to all heavy metals were more than 1.0 at seedling stage. At physiological maturity stage, translocation of Pb, As and Cd in stems and leaves of all hemp varieties were higher compared with Cu and Zn. The accumulated contents of Pb, As, Cu, Cd, and Zn in plants were 31.65-644.29, 365.14-624.25, 180.65-194.06, 15.13-24.40, and 540.07-684.27 g hm -2, respectively. However, highest contents of Pb, As, Cd, and Zn were observed in hemp variety Yunma 1 and Yunma 5, which suggested that these two varieties had significantly higher metal accumulation and translocation compared to other varieties. In conclusion, the bioconcentration ratios, translocation factor and phytoremediation ability of hemp variety Yunma 1 and Yunma 5 were higher than those of Yunma 2, Yunma 3, and Yunma 4. These results showed that both Yunma 1 and Yunma 5 were potential candidates for remediation of heavy metal polluted areas in Yunnan.

Key words: industrial hemp, heavy metals, remediation potential

Fig. 1

Heavy metal contents in roots of different industrial hemp varieties at different stages A: content of Pb in roots; B: content of As in roots; C: content of Cu in roots; D: content of Cd in roots; E: content of Zn in roots. ym1: Yunma 1; ym2: Yunma 2; ym3: Yunma 3; ym4: Yunma 4; ym5: Yunma 5. Bars superscrpted by different lowercase letters indicate significant differences among treatments at the 0.05 probability level."

Fig. 2

Heavy metal contents in stem and leaf of different industrial hemp varieties at different stage A: content of Pb in stems and leaves; B: content of As in stems and leaves; C: content of Cu in stems and leaves; D: content of Cd in stems and leaves; E: content of Zn in stems and leaves. ym1: Yunma 1; ym2: Yunma 2; ym3: Yunma 3; ym4: Yunma 4; ym5: Yunma 5. Bars superscripted by different lowercase letters indicate significant differences among treatments at the 0.05 probability level."

Table 1

Bioconcentration factors (BCF) of heavy metals in different hemp organs at seedling stage"

品种
Variety		 Pb As Cu Cd Zn
根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf
ym1 0.09 a 0.27 a 0.05 b 0.39 a 0.28 c 0.73 a 0.05 c 1.20 a 0.34 b 0.74 a
ym2 0.08 a 0.22 c 0.08 ab 0.41 a 0.32 a 0.81 a 0.09 c 0.93 a 0.40 a 0.61 a
ym3 0.08 a 0.24 b 0.08 ab 0.42 a 0.30 bc 0.83 a 0.03 c 0.96 a 0.38 a 0.69 a
ym4 0.09 a 0.19 d 0.10 a 0.28 b 0.33 a 0.86 a 0.18 b 0.78 a 0.37 a 0.62 a
ym5 0.09 a 0.26 ab 0.09 a 0.42 a 0.31 ab 0.82 a 0.30 a 1.15 a 0.40 a 0.77 a

Table 2

Translocation factor (TF) of heavy metals in different organs at hemp seedling stage"

品种
Variety		 Pb茎叶/根
Pb TF in stem and leaf/root		 As茎叶/根
As TF in stem and leaf/root		 Cu茎叶/根
Cu TF in stem and leaf/root		 Cd茎叶/根
Cd TF in stem and leaf/root		 Zn茎叶/根
Zn TF in stem and leaf/root
ym1 2.93 a 8.68 a 2.65 a 24.88 ab 2.19 a
ym2 2.78 a 5.22 bc 2.53 a 13.81 bc 1.55 b
ym3 2.86 a 5.83 b 2.81 a 32.53 a 1.83 ab
ym4 2.18 b 2.94 c 2.64 a 5.48 c 1.66 ab
ym5 2.93 a 4.96 bc 2.69 a 3.84 c 1.92 ab

Table 3

Bioconcentration factors (BCF) of heavy metals in different organs at hemp process maturity stage"

品种
Variety		 Pb As Cu Cd Zn
根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf		 根
Root		 茎叶
Stem and leaf
ym1 0.22 a 0.26 a 0.15 c 0.51 a 0.32 b 0.24 b 0.65 b 0.92 a 0.42 c 0.45 b
ym2 0.13 c 0.27 a 0.37 a 0.30 c 0.32 b 0.28 a 0.81 ab 0.90 a 0.48 bc 0.65 a
ym3 0.15 bc 0.19 ab 0.23 b 0.36 bc 0.39 ab 0.29 a 0.63 b 0.52 b 0.55 ab 0.42 b
ym4 0.19 ab 0.22 ab 0.36 a 0.41 b 0.48 a 0.26 ab 1.09 a 0.80 a 0.58 ab 0.44 b
ym5 0.17 bc 0.18 b 0.31 a 0.48 a 0.46 ab 0.24 b 0.49 b 0.74 a 0.51 a 0.45 b

Table 4

Translocation factor (TF) of heavy metals in different organs at hemp process maturity stage"

品种
Variety		 Pb茎叶/根
Pb TF of stem and leaf/root		 As茎叶/根
As TF of stem and leaf/root		 Cu茎叶/根
Cu TF of stem and leaf/root		 Cd茎叶/根
Cd TF of stem and leaf/root		 Zn茎叶/根
Zn TF of stem and leaf/root
ym1 1.21 b 3.30 a 0.76 ab 1.52 ab 1.08 b
ym2 2.02 a 0.77 b 0.89 a 1.20 ab 1.36 a
ym3 1.23 b 1.74 b 0.74 ab 0.91 ab 0.77 d
ym4 1.23 b 1.05 b 0.56 b 0.77 b 0.76 d
ym5 1.03 b 1.48 b 0.54 b 1.77 a 0.88 c

Table 5

Absolution accumulation quantity of heavy metals in industrial hemp (g hm-2)"

时期
Stage		 品种
Variety		 Pb As Cu Cd Zn
苗期
Seeding stage		 ym1 43.59 a 27.83 a 31.61 b 2.13 a 96.20 a
ym2 29.10 c 23.51 b 26.56 c 1.32 b 63.61 e
ym3 32.18 b 22.79 b 26.93 c 1.38 b 71.32 d
ym4 21.98 d 16.55 c 13.63 d 1.35 b 22.71 c
ym5 42.84 a 29.88 a 34.41 a 2.05 a 92.87 b
工艺成熟期
Physiological maturity stage		 ym1 644.29 a 624.25 a 194.06 a 24.40 a 669.15 a
ym2 431.65 d 365.14 c 179.67 a 16.64 b 560.11 b
ym3 477.83 c 393.77 c 197.21 a 15.13 b 577.06 b
ym4 440.33 d 371.86 c 180.65 a 16.35 b 540.07 b
ym5 535.77 b 550.76 b 180.94 a 22.60 a 684.27 a
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