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作物学报 ›› 2008, Vol. 34 ›› Issue (03): 369-375.doi: 10.3724/SP.J.1006.2008.00369

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

不同环境条件下稻米组氨酸和精氨酸的胚乳和母体植株QTL分析

郑希1;吴建国1;楼向阳2;徐海明1;石春海1,*   

  1. 1 浙江大学农学系, 浙江杭州310029; 2 Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, VA 22911, USA
  • 收稿日期:2007-09-17 修回日期:1900-01-01 出版日期:2008-03-12 发布日期:2008-03-12
  • 通讯作者: 石春海

Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments

ZHENG Xi1,WU Jian-Guo1,LOU Xiang-Yang2,XU Hai-Ming1,SHI Chun-Hai1*   

  1. 1 Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China; 2 Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, VA 22911, USA

  • Received:2007-09-17 Revised:1900-01-01 Online:2008-03-12 Published:2008-03-12
  • Contact: SHI Chun-Hai

摘要:

利用汕优63重组自交系与双亲回交产生的BC1F1和BC2F1群体,采用新发展的包括环境互作效应在内的多遗传体系QTL作图方法和基因定位软件,对稻米两种半必需氨基酸(组氨酸和精氨酸)进行三倍体胚乳和二倍体母体植株等不同遗传体系的QTL定位分析。共检测到10个控制组氨酸含量的QTL以及8个控制精氨酸含量的QTL。全部QTL均具有极显著的三倍体胚乳和二倍体母体植株基因的加性主效应,其中4个QTL具有显著或极显著的三倍体胚乳显性主效应,7个QTL还具有明显的环境互作效应。

关键词:

水稻(Oryza sativa L.), QTL定位, 组氨酸, 精氨酸, 胚乳, 母体植株, 环境互作效应

Abstract:

As a staple cereal crop in the world, rice feeds more than 50% of the world population. The improvement for rice quality including protein content and kinds of amino acid contents is an important work to meet the demands of a growing population. The genetic analysis on rice indicate that the inheritance of nutrient quality traits is complex, which involve the genetic effects from the triploid endosperm nuclear genes and the diploid maternal plant nuclear genes, being further partitioned into additive and dominance effects, and their genotype × environment (GE) interaction effects. So far, the magnitude and prevalence of interactions between quantitative trait loci (QTL) of the triploid endosperm genome or diploid maternal genome for rice traits are still largely unknown. Therefore, it is necessary to study the genetic main effects and GE interaction effects of the QTL from different genomes across environments. Investigations to identify QTL governing histidine (His) and arginine (Arg) contents of rice were conducted using the newly developed QTL mapping method including endosperm and maternal main effects and their GE interaction effects on quantitative traits of seed in cereal crops. Two backcross populations, which were a set of 241 RILs derived from an elite hybrid cross of ‘Shanyou 63’ crossed with ‘Zhenshan 97’ (BC1F1) or ‘Minghui 63’ (BC2F1), were used in two environments. The results showed that significant differences were found between the two parents for both quantitative quality traits. His and Arg of rice for Zhenshan 97 were higher than those for Minghui 63. The distributions of phenotypic values for His and Arg in BC1F1 (RILs × P1) and BC2F1 (RILs × P2) populations revealed normal distributions approximately. Both backcross populations also showed varying distributions in 1999 and 2000, implying that both amino acid traits were subjected to the modification by environments. A total of ten QTL associated with His content were mapped on chromosomes 1, 2, 3, 6, 7, 10, 11, and 12. Significant additive effects (ae and de) of QTL from diploid maternal plant and triploid endosperm were detected for all of these QTL. Two of them were also found to have visible endosperm dominance main effects and five QTL had significant environmental interaction effects. A total of eight QTL associated with Arg content of rice were mapped on chromosomes 2, 3, 5, 6, 7, 10, 11, and 12. Significant additive main effects of QTL from both genomes were all detected for all of these QTL. Two of them were also found to have visible endosperm dominance main effects and two QTL had significant environmental interaction effects. The proportions of phenotypic variation attributable to the total genetic main effects and GE interaction of QTL were 0.147 and 0.055 for His and 0.160 and 0.018 for Arg, respectively. These results showed that the control for His and Arg contents of rice was distributed over several chromosomes and the environmental interaction effects were also important for the performance of these quality traits.

Key words:

Rice (Oryza sativa L.), Quantitative trait loci (QTL), Histidine, Arginine, Endosperm, Maternal plant, Environmental interaction

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