HSP70可提高干旱高温复合胁迫诱导的玉米叶片抗氧化防护能力

doi:10.3724/SP.J.1006.2010.00636

摘要/Abstract

摘要：

为确定热休克蛋白70 (HSP70)提高作物耐干旱高温复合胁迫的机制，对干旱、高温反应不同的4个玉米品种的生理特性进行了研究。结果显示：(1) 在干旱、高温、干旱高温复合胁迫条件下，叶片丙二醛(MDA)增加量以隆玉602最低，驻玉309最高；在干旱胁迫条件下，郑单958叶片MDA含量低于浚单20，而在高温胁迫条件下，浚单20叶片MDA含量低于郑单958。(2) 在干旱、高温、干旱高温复合胁迫条件下，隆玉602、郑单958、浚单20三个品种叶片抗氧化防护系统如抗坏血酸过氧化物酶(APX)、谷胱苷肽还原酶(GR)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性增加量均高于驻玉309；在干旱条件下，郑单958叶片APX、GR、SOD、CAT活性增加量显著高于浚单20，在高温条件下，两个品种的表现则相反；在干旱高温复合胁迫条件下，隆玉602 叶片APX、GR、SOD活性增加量显著高于郑单958和浚单20。(3) HSP70抑制剂斛皮素(Quercetin, Q)预处理显著抑制了3种胁迫诱导的4个品种叶片抗氧化酶活性的增加。这些研究结果暗示HSP70提高了干旱、高温、干旱高温复合胁迫诱导的抗氧化防护酶活性，且APX、GR、SOD 这3个抗氧化防护酶活性可以作为评价作物耐干旱、高温和干旱高温复合胁迫的生化指标。

关键词: 玉米, 干旱, 高温, 复合胁迫, 热休克蛋白

Abstract:

In order to determine the mechanism of heat shock protein (HSP70) increasing crops endurance to the combination of drought and heat, we investigated the physiological characteristics of four maize varieties with different responses to drought and heat stresses. The results are as follows: (1) Under drought, heat, the combination of drought and heat, the increase of malondialdelehyde (MDA) content in leaf was the lowest in Zhuyu 309 without endurance to three stresses and the highest in Longyu 602 with endurance to three stresses; under drought, leaf MDA increase in Zhengdan958 with drought-endurance was less than that in Xundan 20 leaves with heat-endurance; under heat, leaf MDA increase was more in Zhengdan 958 than in Xundan20. (2) In Zhengdan958, Xundan20, Longyu602 leaves, the increase of ascorbate peroxidese (APX), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT) activities was more than that in Zhuyu 309 leaves under drought, heat, the combination of drought and heat; under drought, APX, GR, SOD, CAT activities of Zhengdan 958 leaves increased significantly more than those of Xundan 20’, but those were contrary under heat; under the combination of drought and heat, APX, GR, SOD activities of Longyu 602 leaves increased significantly more than those of Zhengdan 958 and Xundan 20. (3) The pretreatment with HSP70 inhibitor quercetin (Q) significantly inhibited the increase of antioxidant enzymes activities in leaves of 4 maize varieties exposed to the three stresses. These results suggested that HSP70 involved in the increase of antioxidant enzyme activity in leaves of 4 maize varieties exposed to drought, heat and their combination, and APX, GR, SOD activities could be used as chemical and biological indicators to evaluate the crop endurance to drought, heat, and the combination of drought and heat stresses.

Key words: Maize(Zea mays L.), Drought, Heat, Combination stress, HSP70

胡秀丽,李艳辉,杨海荣,刘全军,李潮海. HSP70可提高干旱高温复合胁迫诱导的玉米叶片抗氧化防护能力[J]. 作物学报, 2010, 36(4): 636-644.

HU Xiu-Li,LI Yan-Hui,YANG Hai-Rong,LIU Qun-Jun,LI Chao-Hai. Heat Shock Protein 70 May Improve the Ability of Antioxidant Defense Induced by the Combination of Drought and Heat in Maize Leaves[J]. Acta Agron Sin, 2010, 36(4): 636-644.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2010.00636

https://zwxb.chinacrops.org/CN/Y2010/V36/I4/636

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