作物学报 ›› 2009, Vol. 35 ›› Issue (9): 1637-1645.doi: 10.3724/SP.J.1006.2009.01637
朱新霞1,2,朱一超1,艾尼江1,刘任重1,张天真1,*
ZHU Xin-Xia1,2,ZHU Yi-Chao1,AI Ni-Jiang1,LIU Ren-Zhong1,ZHANG Tian-Zhen1*
摘要:
以与中棉所12及其2个选系为亲本组配的4个杂交棉中棉所28、中棉所29、湘杂棉2号和冀棉18苗期的根和顶端叶为研究材料,采用cDNA-AFLP技术分析苗期杂交棉与亲本的根和叶基因差异表达,并用Quantitative Real-Time技术加以验证。结果表明:(1)在4个杂交组合中,中棉所12选系是营养生长杂种优势高值亲本;(2)杂交种和亲本间存在显著的基因表达差异,可分为杂交种上调、单亲显性、单亲沉默、杂交种下调4种表达型。4个杂交组合在三叶期根和叶中差异表达基因的4种类型比例趋势基本一致,单亲差异表达型(包括显性和沉默表达)在根和叶中所占比例较高,杂种下调表达型所占比例较低,反映出苗期单亲差异表达型在杂种优势形成中起主要作用;叶部差异表达基因数目和比例(29.20%~46.09%)比根(15.65%~22.49%)高的多,说明叶中基因差异表达可能比根中基因差异表达对杂种优势形成作用更大;(3)高值亲本中棉所12选系与杂交棉共同表达的基因多于低值亲本与杂交棉共同表达的基因,从分子水平上证明中棉所12选系在杂交棉冀棉18、中棉所29和中棉所28的苗期营养生长杂种优势产生中起优势亲本的作用;(4) 4种杂交组合差异表达基因(包含叶和根)占总表达基因的27.00%~34.56%,分析差异表达基因类型和4个杂交组合的关系发现,超显性效应占3.30%~7.17%,超低亲效应占2.62%~4.14%,低亲效应占5.65%~13.03%,显性效应和加性效应是主要的杂种优势效应,占79.52%~83.79%。多种杂种优势效应的并存说明杂种优势可能是多基因共同作用产生多种效应的结果;(5)超亲优势组合中棉所28的超显性效应占7.17%,明显高于其他3个表现中亲优势组合,说明杂交种上调表达型可能对苗期杂种优势产生起重要作用。
[1] Tasftaris S A. Molecular aspects of heterosis in plants. Plant Physiol, 1995, 94: 362-370[2] Sun Q X, Wu L M, Ni Z F, Meng F R, Wang Z K, Lin Z. Differential gene expression patterns in leaves between hybrids and their parental inbreds are correlated with heterosis in a wheat diallel cross. Plant Sci, 2004, 166: 651-657[3] Adams K L, Evolution of duplicate gene expression in polyploid and hybrid plants.J Heredity, 2007, 98: 136-141[4] Tan L-W(谭联望), Liu Z-D(刘正德). Research on selection and varietal traits of Zhongmian 12. Sci Agric Sin (中国农业科学), 1990, 23(3): 12-19 (in Chinese with English abstract )[5] Guo X-M(郭香墨), Tan L-W(谭联望), Liu Z-D(刘正德).Evaluating germplasm resource value of Zhongmian 12.China Cotton (中国棉花), 2002, 29(12): 12-14(in Chinese with English abstract)[6] Bachem C W, van der Hoeven R S, de Bruijn S M, Vreugdenhil D, Zabeau M, Visser R G. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development. Plant J, 1996, 9: 745-753[7] Breyne P, Dreesen R, Cannoot B, Rombaut D, Vandepoele K, Rombauts S, Vanderhaeghen R, Inzé D, Zabeau M. Quantitative cDNA-AFLP analysis for genome-wide expression studies. Mol Genet Genomics, 2003, 269: 173-179[8] Xing C-Z(邢朝柱), Zhao Y-L(赵云雷), Yu S-X(喻树迅), Zhang X-L(张献龙), Guo L-P(郭立平), Miao C-D(苗成朵). Study on gene differential expression in roots and leaves between hybrid CRI47 and its parents at seedling stage. Sci Agric Sin (中国农业科学), 2005, 38(6): 1275-1281(in Chinese with English abstract)[9] Huang Z-K(黄滋康) ed. China Cotton Variety and Family Tree (中国棉花品种及系谱). Beijing: Science Press, 2007 (in Chinese)[10] Jiang J-X(蒋建雄), Zhang T-Z(张天真). Extraction of total RNA in tissues with CTAB- acidic phenolic method. Cotton Sci (棉花学报),2003, 15(3):166-167(in Chinese with English abstract) [11] Sambrook J, Fritsh E F, Maniatis T, eds. Jin D-Y(金冬雁), trans. Molecular Clone Experimental Manual (分子克隆实验指南), 2nd edn. Beijing: Science Press, 1998 (in Chinese)[12] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCtmethod. Methods, 2001, 25: 402-408[13] Pan Y-X(潘玉欣), Ma J(马骏), Zhang G-Y(张桂寅), Han G-Y(韩改英), Wang X-F(王省芬), Ma Z-Y(马峙英). cDNA-AFLP profiling for the fiber development stage of secondary cell wall synthesis and transcriptome mapping in cotton. Chin Sci Bull (科学通报), 2007, 52(17): 2358-2364[14] Li G, Gao M, Yang B, Quiros C F. Gene for gene alignment between Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor Appl Genet, 2003, 107: 168-180[15] Kojima T, Habu Y, Lida S, Ogibara Y. Direct isolation of differentially expressed genes from a specific chromosome region of common wheat-Application the amplified fragment length polymotphism-based messenger-RNA fingerprinting (Amf) method in combination with a deletion line of wheat. Mol Gen Genet, 2000, 263: 635-641[16] Zhang Y(张一), Ni Z-F(倪中福), Yao Y-Y(姚颖垠), Sun Q-X(孙其信). Differential gene expression in uppermost internode between wheat hybrid and its parents. Acta Agron Sin (作物学报), 2008, 34(5): 770-776 (in Chinese with English abstract)[17] Hoecker N, Keller B, Piepho H P, Hochholdinger F. Manifestation of heterosis during early maize (Zea mays L.) root development. Theor Appl Genet, 2006, 112: 421-429[18] Swanson-Wagner R A, Jia Y, DeCook R, Borsuk L A, Nettleton D, Schnable P S. All possible modes of gene action are observed in a global comparison of gene expression in a maize F1-hybrid and its inbred parents. Proc Natl Acad Sci USA, 2006, 103: 6805-6810[19] Meyer S, Pospisil H, Scholten S. Heterosis associated gene expression in maize embryos 6 days after fertilization exhibits additive, dominant and overdominant pattern. Plant Mol Biol, 2007, 63: 381-391[20] Hoecker N, Keller B, Muthreich N, Chollet D, Descombes P, Piepho H P, Hochholdinger F. Comparison of maize (Zea mays L.) F1-hybrid and parental inbred line primary root transcriptomes suggests organ-specific patterns of nonadditive gene expression and conserved expression trends. Genetics, 2008, 179: 1275-1283 [21] Sun Q X, Ni Z F, Liu Z Y. Differential gene expression between wheat hybrids and their parental inbreds in seedling leaves. Euphytica, 1999, 106: 117-123[22] Xie X-D(谢晓东), Ni Z-F(倪中福), Meng F-R(孟凡荣), Wu L-M(吴利民), Wang Z-K(王章奎), Sun Q-X(孙其信). Relationship between difference of gene expression in early developing seed of hybrid versus parents and heterosis in wheat. Acta Genet Sin (遗传学报), 2003, 30(3): 260-266(in Chinese with English abstract)[23] Wang Z-K(王章奎), Ni Z-F(倪中福), Meng F-R(孟凡荣), Wu L-M(吴利民), Xie X-D(谢晓东), Sun Q-X(孙其信). Primary study on the relationship between differential gene expression pattern in roots at jointing stage and heterosis in agronomic traits in a wheat diallel cross. Sci Agrci Sin (中国农业科学), 2003, 36(5): 473-479 (in Chinese with English abstract)[24] Tian Z-Y(田曾元), Dai J-R(戴景瑞). Relationship between differential gene expression patterns in functional leaves of maize inbreds and hybrids at spikelet differentiation stage and heterosis. Acta Genet Sin (遗传学报), 2003, 30(2): 154-162 (in Chinese with English abstract)[25] Xiong L Z, Yang G P, Xu C G, Zhang Q F. Relationships of differential gene expression in leaves with heterosis and heterozygosity in a rice diallel cross. Mol Breed, 1998, 4: 129-136 [26] Xing C-Z(邢朝柱), Yu S-X(喻树迅), Zhao Y-L(赵云雷), Guo L-P(郭立平), Zhang X-L(张献龙), Miao C-D(苗成朵), Wang H-L(王海林). Primary study on gene differential expression of Pest-resistant cotton hybrids between different heterosis crosses. Acta Agron Sin (作物学报), 2007, 33(3): 507-510(in Chinese with English abstract)[27] Xing C-Z(邢朝柱), Zhao Y-L(赵云雷), Yu S-X(喻树迅), Zhang X-L(张献龙), Guo L-P(郭立平), Wang H-L(王海林). Relationship between gene differential expression of leaves in full opening flower stages of hybrids& their parents heterosis in pest-resistant cotton. Acta Genet Sin (遗传学报), 2006, 33(10): 948-956[28] Wu M-S(吴敏生), Gao Z-H(高志环), Dai J-R(戴景瑞). Studies on differential gene expression of maize (Zea mays L.) by means of cDNA-AFLP. Acta Agron Sin (作物学报), 2001, 27(3): 339-342 (in Chinese with English abstract)[29] Wu L-M(吴利民), Ni Z-F(倪中福), Wang Z-K(王章奎). Relationship between differential expression patterns of multigene families and heterosis in a wheat diallel crosses. Acta Genet Sin (遗传学报), 2001, 28(3): 256-266 (in Chinese with English abstract)Wang Z, Ni Z, Wu H, Nie X, Sun Q.Heterosis in root development and differential gene expression between hybrids and their parental inbreds in wheat (Triticum aestivum L.). Theor Appl Genet, 2006, 113: 1283-1294 |
[1] | 李永辉,陈琳琳,孙炳剑,王利民,邢小萍,袁虹霞,丁胜利*,李洪连*. 假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析[J]. 作物学报, 2017, 43(11): 1632-1642. |
[2] | 谭河林,许欣颖,付立曼,向小娥,李剑桥,郭昊伦,叶文雪. 甘蓝型油菜及其亲本物种甲基化酶I基因的克隆及表达模式[J]. 作物学报, 2015, 41(03): 405-413. |
[3] | 冯国艺,干秀霞,杨美森,姚炎帝,罗宏海,张亚黎,张旺锋. 两个杂交棉F1、F2代及亲本冠层结构与物质生产特征[J]. 作物学报, 2013, 39(09): 1635-1643. |
[4] | 张亚黎,冯国艺,胡渊渊,姚炎帝,张旺锋. 棉花非叶绿色器官光合能力的差异及与物质生产的关系[J]. 作物学报, 2010, 36(4): 701-708. |
[5] | 杜明伟,冯国艺,姚炎帝,罗宏海,张亚黎,夏东利,张旺锋. 杂交棉标杂A1和石杂2号超高产冠层特性及其与群体光合生产的关系[J]. 作物学报, 2009, 35(6): 1068-1077. |
[6] | 朱新霞,汪保华,郭旺珍,张天真. 中棉所12配制的2个杂交棉DNA甲基化遗传与传递[J]. 作物学报, 2009, 35(12): 2150-2158. |
[7] | 李永春;孟凡荣;王潇;陈雷;任江萍;牛洪斌;李磊;尹钧. 水分胁迫条件下“洛旱2号”小麦根系的基因表达谱[J]. 作物学报, 2008, 34(12): 2126-2133. |
[8] | 张一;倪中福;姚颖垠;孙其信. 小麦杂交种与亲本之间穗下节间基因差异表达分析[J]. 作物学报, 2008, 34(05): 770-776. |
[9] | 邢朝柱 ; 喻树迅 ; 赵云雷; 郭立平 ; 张献龙; 苗成朵 ; 王海林. 不同优势抗虫棉杂交组合不同生育期基因表达差异初探[J]. 作物学报, 2007, 33(03): 507-510. |
[10] | 陈源;顾万荣;陈德华;田明军;夏文省;周芳刚;季春梅;吴云康. 转基因抗虫棉杂交种育苗移栽高产栽培途径的研究[J]. 作物学报, 2005, 31(04): 487-492. |
|