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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1265-1269.doi: 10.3724/SP.J.1006.2010.01265

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦品系天95HF2抗叶锈基因定位

周悦,王龙,张晔,李在峰*,刘大群*   

  1. 河北农业大学植物保护学院 / 河北省农作物病虫害生物防治工程技术研究中心，河北保定 071001
  • 收稿日期:2010-03-15 修回日期:2010-04-24 出版日期:2010-08-12 网络出版日期:2010-06-11
  • 通讯作者: 刘大群, E-mail: ldq@hebau.edu.cn, Tel: 0312-7528500; 李在峰, E-mail: lzf7551@yahoo.com.cn, Tel: 0312-7528500
  • 基金资助:
     本研究由国家自然科学基金(30971772, 30700505)和国家“十一五”科技支撑计划项目(2006BAD08A05)资助。

Molecular Mapping of Leaf Rust Resistance Genes in Wheat Line Tian 95HF2

ZHOU Yue,WANG Long,ZHANG Ye,LI Zai-Feng*,LIU Da-Qun*   

  1. College of Plant Protection, Agricultural University of Hebei / Biological Control Center for Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China
  • Received:2010-03-15 Revised:2010-04-24 Published:2010-08-12 Published online:2010-06-11
  • Contact: LIU Da-Qun, E-mail: ldq@hebau.edu.cn, Tel: 0312-7528500;LI Zai-Feng, E-mail: lzf7551@yahoo.com.cn, Tel: 0312-7528500

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1451

被引次数

26

摘要： 苗期基因推导表明，小麦品系天95HF2高抗我国目前多数叶锈菌生理小种。为了确定这一品系所携带的抗病基因，以天95HF2和感病小麦品种郑州5389杂交，获得F1和F2代群体，用叶锈菌小种FHTT和PHTS分别对双亲及其杂交后代进行叶锈抗性鉴定并进行分子标记分析。结果表明，用叶锈菌小种FHTT接种F2代群体时呈现1对显性基因的抗感分离比例，经过亲本和抗感池间标记筛选以及F2代群体的标记检测，Lr1的STS标记WR003和位于5DL的SSR标记wmc443与该抗病基因连锁，遗传距离分别为2.9 cM和3.1 cM，根据抗性特点和染色体位置推断该基因可能为Lr1。用叶锈菌小种PHTS接种F2代群体时呈现2对基因的抗感分离，分子标记分析结果表明，其中一个基因为Lr1，另一个基因可能为LrZH84。

关键词: SSR标记, 小麦抗叶锈基因, 天95HF2, Lrl, LrZH84

Abstract: The wheat (Triticum aestivum L.) line Tian 95HF2 appears in low infection to most of Chinese current pathotypes of Puccinia triticina at seedling stage. For postulating the resistance genes in Tian 95HF2, F1 and F2 populations from the cross between Tian 95HF2 and Zhengzhou 5389 (susceptible to leaf rust) were inoculated with pathotypes FHTT and PHTS in greenhouse. The infection types were investigated 15 d after inoculation and molecular markers were also used for mapping the resistance genes. When inoculating with pathotype FHTT, only one resistance gene was detected. After screening 1274 primer pairs in the parents and the resistant and susceptible bulks, the resistance gene was found between the interval of a sequence tag site for Lr1 (WR003) and a microsatellite (wmc443) on 5DL.The genetic distances to the two markers were 2.9 cM and 3.1 cM, respectively. This resistance gene was finally postulated to be Lr1. The segregation of infection type in response to pathotype PHTS showed that there were two resistance genes in Tian 95HF2. One was inferred as Lr1, and the other was likely to be LrZH84, which is located on chromosome 1BL.

Key words: SSR marker, Resistance gene to wheat leaf rust, Tian 95HF2, Lrl, LrZH84

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