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作物学报 ›› 2011, Vol. 37 ›› Issue (01): 87-94.doi: 10.3724/SP.J.1006.2011.00087

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

ET-ISJ标记的开发及陆地棉遗传图谱构建

林刚,张建,张轲,藤中华,张正圣*   

  1. 西南大学农学与生物科技学院 / 农业部生物技术与作物品质改良重点开放实验室，重庆400716
  • 收稿日期:2010-04-16 修回日期:2010-07-29 出版日期:2011-01-12 网络出版日期:2010-10-09
  • 通讯作者: 张正圣, E-mail: zhangzs@swu.edu.cn, Tel: 13883608797
  • 基金资助:

    本研究由国家自然科学基金项目(30871556, 30900910)和国家高技术研究发展计划(863计划)项目(2006AA10Z1D3)资助。

Development of Exon-Targeted Intron-Exon Splicing Conjunction (ET-ISJ) Marker and Establishment of Upland Cotton Genetic Map

LIN Gang,ZHANG Jian,ZHANG Ke,TENG Zhong-Hua,ZHANG Zheng-Sheng*   

  1. College of Agronomy and Biotechnology / Key Laboratory of Biotechnology & Crop Quality Improvement, Ministry of Agriculture, Southwest University, Chongqing 400716, China
  • Received:2010-04-16 Revised:2010-07-29 Published:2011-01-12 Published online:2010-10-09
  • Contact: ZHANG Zheng-Sheng,E-mail:zhangzs@swu.edu.cn,Tel:13883608797

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1971

被引次数

26

摘要： 根据植物结构基因外显子拼接位点的保守序列，设计扩增外显子的ET-ISJ (exon targeted intron-exon splice junction)标记引物。利用1 280对ET-ISJ引物组合，在陆地棉品种渝棉1号和T586中，筛选获得69对多态性引物组合，占引物组合的5.4%。用多态性ET-ISJ引物组合检测(渝棉1号×T586)F2:7重组近交系群体，得到70个位点。以70个ET-ISJ标记位点与523个SSR、59个IT-ISJ、29个SRAP和8个形态标记进行连锁分析，构建的遗传连锁图谱包括59个连锁群和673个位点(68个ET-ISJ、510个SSR、58个IT-ISJ、29个SRAP和8个形态标记)。连锁图覆盖3 216.7 cM，占棉花基因组的72.3%，标记间平均长度为4.8 cM。68个ET-ISJ标记分布于20条染色体。研究表明ET-ISJ标记多态性较高、稳定性好，可有效用于棉花与其他植物遗传连锁图谱构建。

关键词: 陆地棉, 外显子定靶内含子-外显子剪接位点标记, 遗传图谱

Abstract: Cotton is the leading fiber crop in the world, and upland cotton contributes over 95% of cotton production. The genetic map of upland cotton is far from saturated, so it is necessary to develop new markers for it. ET-ISJ (exon targeted intron-exon splice conjunctions) maker primers were designed according to the conserved intron-exon splicing junction sequences. A total of 1 280 ET-ISJ primer combinations were used to screen polymorphism between upland cotton cultivar Yumian 1 and T586, and 69 of which showed polymorphism, accounting for 5.4% of total primer combinations. The 69 polymorphic ET-ISJ primer combinations were used to detect (Yumian 1 × T586) F2:7 recombinant inbred line population, and 70 ET-ISJ loci were obtained. Linkage analysis was conducted on 70 ET-ISJ loci, 523 SSR, 59 IT-ISJ, 29 SRAP and 8 morphological loci, and a linkage map including 59 linkage groups and 673 loci (68 ET-ISJ, 510 SSR, 58 IT-ISJ, 29 SRAP and 8 morphological loci) was established. The linkage map covered 3 216.7 cM, accounting for 72.3% of cotton genome, with an average interval of 4.8 cM between two markers. Sixty-eight ET-ISJ loci were located on 20 chromosomes.The present study demonstrated that ET-ISJ markers are stable, relatively-high polymorphic, and able to be effectively applied in genetic map construction of cotton and other plant’s.

Key words: Upland cotton (Gossypium hirsutum L.), ET-ISJ, Genetic map

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