山药块茎发育中物质积累及相关代谢酶变化

doi:10.3724/SP.J.1006.2011.00903

摘要/Abstract

摘要： 以桂淮5号和桂淮16两个不同山药品种为材料，根据块茎生长发育特性，在山药发育进程中测定块茎的有关生理生化指标。结果表明，随着块茎发育进程的推进，块茎长度及干物质积累持续增加，淀粉酶(AMY)活性降低；多数碳水化合物、功能性物质及过氧化物酶(POD)、酸性转化酶(AI)和蔗糖磷酸合酶(SPS)活性先持续增加，达到峰值后下降，但峰期不同；2个品种的还原糖、ADPG焦磷酸化酶(AGPase)变化不一致。t检验表明，在块茎发育全过程中，品种间的干物质(P=0.011)、淀粉(P=0.031)、皂苷(P=0.019)差异显著。可溶性糖(r₅=0.97^**，r₁₆=1.00^**)、AGPase (r₅= –0.85^*)、还原糖(r₁₆=0.87^*)与蔗糖显著相关；多糖(r₅=0.95^**)、Vc(r₅=0.83^*)、皂苷(r₁₆=0.88^**)与淀粉显著相关；干物质(r₅=0.97^**，r₁₆=0.87^*)、AMY(r₁₆= –0.95^**)、AGPase (r₁₆=–0.90^**)、蛋白质(r₁₆=0.83^*)与块茎伸长膨大显著相关。由此表明，蔗糖积累、SPS和AI活性在山药块茎发育中起关键调控作用，主要功能性物质彼此间密切相关。

关键词: 山药, 块茎发育, 物质积累

Abstract: Tuber development is a complex physiological process, involving in changes of various components and enzymes activities, so learning about changes of the matter accumulation and enzymes metabolism will help to instruct yam production. In the paper, two different cultivars Guihuai 5 and Guihuai 16 were used to investigate some physiological and biochemical indicators during yam tuber development in 2009—2010. The results showed that tuber length and dry matter content were kept increasing, but AMYenzyme activity decreasingduring the tuber development. The main carbohydrates contents, functional substances, and the activities of POD, AI, and SPS presented similar trend: continually increased firstly, and reached various peaks, then decreased. The changes of reducing sugar content and AGPase activity were different between two cultivars. The t-test results indicated that there was a significant difference between two cultivars in dry matter (P=0.011),starch (P=0.031), and saponin (P=0.019). Sucrose content was significantly correlated with soluble sugars content (r₅=0.97^**, r₁₆=1.00^**), AGPase activity (r₅= –0.85^*) and reducing sugars content (r₁₆=0.87^*), and starch content was significantly correlated with polysaccharides (r₅=0.95^**), Vc (r₅=0.83^*) and saponin (r₁₆=0.88^**) contents as well as tuber length was significantly correlated with dry matter content (r₅=0.97^**, r₁₆=87^*), AMY activity (r₁₆= –0.95^**),AGPase activity (r₁₆= –0.90^**) andprotein content (r₁₆=0.83^*). To sum up, the sucrose accumulation, SPS and AIactivity play a key regulatory effect in yam tuber development,the main functional substances are close correlated between each other.

Key words: Yam, Tuber development, Matter accumulation, Enzyme

梁任繁, 李创珍, 张娟, 何龙飞, 韦本辉, 甘秀芹, 何虎翼. 山药块茎发育中物质积累及相关代谢酶变化[J]. 作物学报, 2011, 37(05): 903-910.

LIANG Lin-Fan, LI Chuang-Zhen, ZHANG Juan, HE Long-Fei, HUI Ben-Hui, GAN Xiu-Qin, HE Hu-Yi. Changes of Matter Accumulation and Relative Enzymatic Activity during Yam Tuber Development[J]. Acta Agron Sin, 2011, 37(05): 903-910.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2011.00903

https://zwxb.chinacrops.org/CN/Y2011/V37/I05/903

参考文献

[1]Wei B-H(韦本辉), Tang R-H(唐荣华), Wei W-T(韦威泰), He X-M(何新民), Jiang J(蒋菁). Guangxi Yam (Dioscorea opposita Thunb.) production status and developing ways. Guangxi Agric Sci (广西农业科学), 2003, (1): 7–8 (in Chinese with English abstract)
[2]Yuan S-L(袁书林). Research advances on chemical compositions and bioactivities of Dioscorea Opposita Thunb. (Chinese Yam). Food Res Dev (食品研究与开发), 2008, 29(3): 176–178 (in Chinese with English abstract)
[3]Li L-J(李良俊), Zhang X-D(张晓冬), Pan E-C(潘恩超), Sun L(孙磊), Xie K(谢科), Gu L(顾丽), Cao B-S(曹碚生). Relationship of starch synthesis with its related enzymes activities during rhizome development of lotus. Sci Agric Sin (中国农业科学), 2006, 39(11): 2307–2312 (in Chinese with English abstract)
[4]Wang W(王玮), Gong Y-Q(龚义勤), Liu L-W(柳李旺), Wang Y(王燕), Jing Z-G(荆赞革), Huang D-Q(黄丹琼), Wang L-Z(汪隆植). Changes of sugar content and sucrose metabolizing enzyme activities during fleshy TaProot development in radish (Raphanus sativus L.). Acta Hort Sin (园艺学报), 2007, 34(5): 1313–1316 (in Chinese with English abstract)
[5]Zhao B(赵冰), Zhang C-R(张春荣), Li J-M(李建明), Wu G-Q(吴国强). The comparative study on the pipe culture technique and half pipe culture technique of Dioscorea opposita Thunb. Spec Wild Econ Anim Plant Res (特产研究), 2003, (4): 19–21 (in Chinese with English abstract)
[6]Li M-J(李明军), Liu X-Y(刘欣英), Li P(李萍), Zhang X-L(张晓丽), Zhao X-T(赵喜亭), Liu J(柳俊), Xie C-H(谢从华). Impact factors in vitro induction of microtubers from Dioscorea opposita. Chin Trad Herbal Drubs (中草药), 2008, 39(6): 905–910 (in Chinese with English abstract)
[7]Gao J-F(高俊凤). Experimental Guidance for Plant Physiology (植物生理学实验指导). Beijing: Higher Education Press, 2006. pp 14–15, 144–148(in Chinese)
[8]Wang J-Y(王晶英), Ao H(敖红), Zhang J(张杰). Experimental Principle and Technology of Plant Physiological and Biochemical (植物生理生化实验技术与原理). Harbin: Northeast Forestry University Press, 2003. pp 2–3, 9–11, 22–23 (in Chinese)
[9]Jiang F-T(姜芳婷), Li M-J(李明静), Shi H-Q(史会齐), Liu X-H(刘绣华). Determination of polysaccharide in Dioscorea Opposita Thunb and Dioscorea Alata L. Chem Res (化学研究), 2004, 15(3): 47–49 (in Chinese with English abstract)
[10]Li Z-Y(李朝阳), Yang Z-X(杨朝霞), Chen G-X(陈功锡), Liu H-B(刘辉兵), Li H-M(李鹄鸣). Studies on the growth and morphological variation of leaves and sapogenin content of Dioscorea zingiberensis C. H. Wright from different populations. Life Sci Res (生命科学研究), 2003, 7(1): 58–62 (in Chinese with English abstract)
[11]Bao S-D(鲍士旦). Soil and Agricultural Chemistry Analysis (土壤农化分析). Beijing: China Agriculture Press, 2000. pp 253–254 (in Chinese)
[12]Huang C C, Chiang P Y, Chen Y Y, Wang C C R. Chemical compositions and enzyme activity changes occurring in yam (Dioscorea alata L.) tubers during growth. LWT-Food Sci Technol, 2007, 40: 1498–1506
[13]Gong R-G(龚荣高), Zhang G-L(张光伦), Lü X-L(吕秀兰), Zeng X-L(曾秀丽), Luo N(罗楠), Hu Q(胡强). Studies on the sucrose-metabolizing enzymes in navel orange fruit from different habitat. Acta Hort Sin (园艺学报), 2004, 25(6): 719–722 (in Chinese with English abstract)
[14]Li L-J(李良俊), Xu C(许超), Chen J-L(陈建林), Cao B-S(曹碚生). Changes of carbohydrates during lootus rhizome development of Nelumbo Nucifera Gaertn. J Yangzhou Univ (Agric Life Sci Edn)(扬州大学学报•农业与生命科学版), 2003, 24(3): 72–78 (in Chinese with English abstract)
[15]Sturm A, Liehard S, Schatt S. Tissue specific expression of two genes for sucrose synthase in carrot(Daucus carota L.). Plant Mol Biol, 1999, 39: 349–360
[16]Zamski E, Barnea A. Characterization of sucrose phosphate synthase sucrose synthase and invertase in roots of two nearly isogenic carrot lines that differ in their capacity to accumulate sucrose. Plant Physiol, 1996, 149: 301–306
[17]Lisinska G, Leszczynski W. Potato science and technology. London: Elsevier Applied Science Press, 1989. pp 16–43, 166–233
[18]Farrar J, Pollock C, Gallagher J. Sucrose and the integration of metabolism in vascular plants. Plant Sci, 2000, 154: 1–11
[19]Bachelier C, Granam J, Manoir J. Integration of an invertase gene to control sucrose metabolism in strawberry cultivars. Acta Hort, 1997, 439: 161–163
[20]Klann E M, Hall B, Bennett A B. Antisense acid invertase (TIV1) gene alters soluble sugar composition and size in transgenic tomato fruit. Plant Physiol, 1996, 112: 1321–1330
[21]Zhang W-M(张卫明), Shan C-Y(单承莺), He H-L(何海玲), Jiang H-F(姜洪芳), Zhang J(张玖). Quality comparison between two cultivated Chinese Kunming yam and Tiegun yam. J Chin Med Materials (中药材), 2007, 30(5): 513–515(in Chinese with English abstract)
[22]Xie C-X(谢彩侠), Gao S-L(高山林), Zhang Z-Y(张重义), Huang X-S(黄晓书). Analysis of the chemical components and isomorphic amylase among different local cultivars of Dioscorea opposita. J Plant Res Environ (植物资源与环境学报), 2004, 13(2): 21–24 (in Chinese with English abstract)
[23]Cui X-M(崔秀明), Dong T-X(董婷霞), Chen Z-J(陈中坚), Wang C-L(王朝梁), ZHan H-Q(詹华强). Studies on the change of polysaccharides in Panax notoginseng collected at different times and from regions. Chin Pharmaceutical J (中国药学杂志), 2002, 37(11): 820 (in Chinese with English abstract)
[24]Liang Q-Y(梁秋云), Liu H-G(刘华钢), Lai M-X(赖茂样), Huang H-X(黄慧学). Study of determination of fibrous root of its radix. J Guangxi Med Univ (广西医科大学学报), 2006, 23(1): 36-38(in Chinese with English abstract)
[25]Fan C-F(范昌发), Guo X-C(郭骁才), Jia J-F(贾敬芬), Niu T-T(牛天堂). Relationship of starch metabolism and determination of morphogenetic pathway of plant cells cultured in vitro. Acta Agric Boreali-Sin (华北农学报), 2000, 15(4): 52–57 (in Chinese with English abstract)
[26]Nie L-H(聂凌鸿), Ning Z-X(宁正祥). Study on purification and chemical structure of polysaccharides from Dioscorea ford II. Chem Ind Fort Prod (林产化学与工业), 2004, 24(8): 101–106 (in Chinese with English abstract)
[27]Kang J-H(康建宏), Zhang H-L(张海林), Wu H-L(吴宏亮), Yang J(杨涓), Yang J-T(杨剑涛). Relationships between the main secondary metabolite and polysaccharids of Lycium barbarum fruits at different tree ages. J Agric Sci (农业科学研究), 2008, 29(4): 10–12 (in Chinese with English abstract)
[28]Li X(李祥), Ma J-Z(马建中), Shi Y-D(史云东). Research progress and prospects of Dioscorea and Diosgeni. Chem Ind For Prod (林产化学与工业), 2010, 30(2): 107–112 (in Chinese with English abstract)

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