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作物学报 ›› 2017, Vol. 43 ›› Issue (12): 1827-1834.doi: 10.3724/SP.J.1006.2017.01827

• 耕作栽培·生理生化 • 上一篇    下一篇

小角X射线散射波谱的定量作图分析法在作物淀粉研究中的应用

何巍1,范孝旭1,王志峰2,韦存虚1,*   

  1. 1 扬州大学 / 江苏省作物遗传生理国家重点实验室培育点 / 粮食作物现代产业技术协同创新中心,江苏扬州 225009;2 扬州大学测试中心,江苏扬州 225009
  • 收稿日期:2017-01-09 修回日期:2017-09-10 出版日期:2017-12-12 网络出版日期:2017-09-28
  • 通讯作者: 韦存虚, E-mail: cxwei@yzu.edu.cn, Tel: 0514-87997217
  • 基金资助:

    本研究由国家自然科学基金项目(31570324)和江苏省高校优势学科建设工程项目资助。

Application of Quantitative Graphical Method Based on Small Angle X-Ray Scattering Spectrum in Crop Starch Study

HE Wei1,FAN Xiao-Xu1,WANG Zhi-Feng2,WEI Cun-Xu1,*   

  1. 1 Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; 2 Testing Center, Yangzhou University, Yangzhou 225009, China
  • Received:2017-01-09 Revised:2017-09-10 Published:2017-12-12 Published online:2017-09-28
  • Contact: 韦存虚, E-mail: cxwei@yzu.edu.cn, Tel: 0514-87997217
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31570324) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘要:

用小角X射线散射(small-angle X-ray scattering, SAXS)波谱可以定量分析淀粉的片层结构,但缺乏波谱数据分析软件而且需要专业的数学公式推导,严重制约了SAXS在淀粉研究中的应用。本文建立了一种简单的作图分析方法,可定量测定SAXS波谱中的淀粉片层结构信息(峰强度、峰位置、半峰宽度和片层距离)。我们用该方法定量测定了不同晶体类型淀粉、不同直链淀粉含量的水稻淀粉、酸不溶淀粉和热不溶淀粉的SAXS波谱参数。结果表明,片层结构与淀粉的植物来源有关,而与淀粉晶体类型没有直接的相关性。相同植物来源的淀粉,其直链淀粉含量与SAXS峰强度和半峰宽度呈显著负相关而与片层距离呈正相关。酸水解不影响淀粉片层厚度,但明显改变片层峰强度和半峰宽度。加热处理也不影响淀粉片层厚度,但破坏淀粉晶体结构导致片层峰强度随温度升高逐渐降低直至消失。该定量作图分析法操作简单、重复性好、可信度高,可以在作物淀粉研究中广泛应用。

关键词: 作物淀粉, 小角X射线散射波谱, 波谱参数, 定量作图分析法, 片层结构

Abstract:

Small-angle X-ray scattering (SAXS) spectrum can be used to quantify the lamellar structure of starch. However, the spectrum analysis lacks a special data analysis software and needs complicated mathematical equations for it, which seriously restricts the applications of SAXS technology in starch studies. In this study, a simple graphical method was established to quantitatively measure the SAXS spectrum parameters (peak intensity, peak position, peak width at half maximum, and lamellar distance). These parameters can reflect the information of starch lamellar structure. The SAXS spectra of starch with different crystal types, rice starch with different amylose contents, and acid-modified and gelatinized starch were all analyzed using the graphical method, showing that the lamellar structure of starch was related with the plant origin, but had no direct relationship with crystal types. For the starch from the same plant origin, the amylose content was significantly negatively correlated with peak intensity and peak width at half maximum, and positively correlated with lamellar distance of SAXS spectrum. The acid hydrolysis had no effect on lamellar distance of starch, but changed the peak intensity and peak width at half maximum of SAXS spectrum. Heating treatment also had no effect on lamellar distance of starch, but destroyed the crystalline structure, leading to the gradual decrease, even disappearance of peak intensity with increasing heating temperature. This study indicated that the quantitative graphical method is simple, good repeatable, and highly credible, and could be widely used in crop starch studies.

Key words: Crop starch, Small-angle X-ray scattering spectrum, Spectrum parameter, Quantitative graphical method, Lamellar

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