作物学报 ›› 2018, Vol. 44 ›› Issue (02): 177-184.doi: 10.3724/SP.J.1006.2018.00177
蒲敏1, 罗绍兰1, 廉小平2, 张贺翠1, 白晓璟1, 王玉奎1, 左同鸿1, 高启国2, 任雪松2, 朱利泉1,*()
Min PU1, Shao-Lan LUO1, Xiao-Ping LIAN2, He-Cui ZHANG1, Xiao-Jing BAI1, Yu-Kui WANG1, Tong-Hong ZUO1, Qi-Guo GAO2, Xue-Song REN2, Li-Quan ZHU1,*()
摘要:
自交不亲和性是甘蓝在长期进化过程中形成的防止自交衰败、促进杂交优势的一种复杂而完善的遗传机制。克隆自交不亲和性相关基因对甘蓝自交不亲和性的深入研究和利用有重要意义。本研究通过挖掘0~60 min自花和异花授粉的甘蓝柱头转录组数据, 筛选到一个受自花授粉诱导上调表达的基因, 命名为BoSPI。BoSPI开放阅读框534 bp, 编码177个氨基酸, 理论等电点为4.21, 不包含信号肽和跨膜区, 含有4个保守的EF-hand结构域。BoSPI基因起始密码子上游2000 bp的核苷酸序列中含有真菌诱导响应、代谢调节以及器官形成等应答元件。BoSPI基因在大肠杆菌中可诱导表达为17 kD的蛋白。BoSPI在柱头中表达量最高, 在花瓣、萼片、叶片、雄蕊表达量较低。BoSPI蛋白被定位在细胞膜和细胞质。自花授粉30 min后对BoSPI基因的诱导表达显著增强。表明BoSPI参与了柱头响应自花花粉刺激的分子过程, 可能是实现甘蓝自交不亲和性相关的某种新功能基因。
[1] | Chapman L A, Goring D R.Pollen-pistil interactions regulating successful fertilization in the Brassicaceae.J Exp Bot, 2010, 61: 1987-1999 |
[2] | Vanoosthuyse V, Tichtinsky G, Dumas C, Gaude T, Cock M.Interaction of calmodulin, a sorting nexin and kinase-associated protein phosphatase with theBrassica oleracea S locus receptor kinase. Plant Physiol, 2003, 133: 919-929 |
[3] | Kachroo A, Schopfer C R, Nasrallah M E, Nasrallah J B.Alelle-specific receptor-ligand interactions inBrassica self incompatibility. Science, 2001, 293: 1824-1826 |
[4] | Takayama S, Shimosato H, Shiba H, Funato M, Iwano M, Che F S, Watanabe M, Iwano M, Isogai A.Direct ligand-receptor complex interaction controls Brassica self-incompatibility.Nature, 2001, 413: 534-538 |
[5] | Newbigin E, Vierstra R D.Plant reproduction: sex and self-denial. Nature, 2003, 423: 229-230 |
[6] | 朱利泉, 周燕. 甘蓝自交不亲和性信号传导元件与传导过程. 作物学报, 2015, 41: 1-14 |
Zhu L Q, Zhou Y.Protein elements and signal transduction process of self-incompatibility inBrassica oleracea. Acta Agron Sin, 2015, 41: 1-14 (in Chinese with English abstract) | |
[7] | Nasrallah J B, Nasrallah M E.Robust self-incompatibility in the absence of a functionalARC1 gene in Arabidopsis thaliana. Plant Cell, 2014, 26: 3838-3841 |
[8] | Kitashiba H, Liu P, Nishio T, Nasrallah J B, Nasrallah M E.Functional test ofBrassica self-incompatibility modifiers in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2011, 108: 18173-18178 |
[9] | 贾新平, 叶晓青, 梁丽建, 邓衍明, 孙晓波, 佘建明. 基于高通量测序的海滨雀稗转录组学研究. 草业学报, 2014, 23: 242-252 |
Jia X P, Ye X Q, Liang L J, Deng Y M, Sun X B, She J M.Transcriptome characteristics ofPaspalum vaginatum analyzed with illumina sequencing technology. Acta Pratac Sin, 2014, 23: 242-252 (in Chinese with English abstract) | |
[10] | Mortazavil A, Williams B A, McCue1 K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq.Nat Methods, 2008, 5: 621-628 |
[11] | Skelton N J, Kördel J, Akke M, Chazin W.Signal transduction versus buffering activity in Ca2+-binding proteins. Nat Struct Mol Biol, 1994, 1: 239-245 |
[12] | Ikura M.Calcium binding and conformational response in EF-hand proteins. Trends Biochem Sci, 1996, 21: 14-17 |
[13] | Kretsinger R H.EF-hands embrace. Nat Struct Mol Biol, 1997, 4: 514-516 |
[14] | Chen C, Sun X L, Duanmu H Z, Zhu D, Yu Y, Cao L, Liu A, Bowei J, Xiao J L, Zhu Y Z.GsCML27, a gene encoding a calcium-binding EF-hand protein fromGlycine soja, plays differential roles in plant responses to bicarbonate, salt and osmotic stresses. PLoS One, 2015, 10: e0141888 |
[15] | 于晓俊, 曹绍玉, 董玉梅, 毕保良, 张应华, 许俊强. 钙结合蛋白对花粉生长发育调控研究进展. 西北植物学报, 2016, 36: 2121-2127 |
Yu X J, Cao S Y, Dong Y M, Bi B L, Zhang Y H, Xu J Q.Research progress of calcium binding proteins in pollen growth and development.Acta Bot Boreali-Occident Sin, 2016, 36: 2121-2127 (in Chinese with English abstract) | |
[16] | Zonia L, Munnik T.Uncovering hidden treasures in pollen tube growth mechanics.Trends Plant Sci, 2009, 14: 318-327 |
[17] | Iwano M, Shiba H, Miwa T, Che F S, Takayama S, Nagai T, Miyawaki A, Lsogai A.Ca2+ dynamics in a pollen grain and papilla cell during pollination of Arabidopsis.Plant Physiol, 2004, 136: 3562-3571 |
[18] | Lazzaro M D, Cardenas L, Bhatt A P, Justus C D, Phillips M S, Holdaway-Clarke T L, Hepler P K. Calcium gradients in conifer pollen tubes, dynamic properties differ from those seen in angiosperms. J Exp Bot, 2005, 56: 2619-2628 |
[19] | Guan Y F, Gu J Z, Li H, Li H, Yang Z B.Signaling in pollen tube growth: crosstalk, feedback and missing links.Mol Plant, 2005, 6: 1053-1064 |
[20] | Michard E, Lima P T, Borges F, Silva A C, Portes M T, Carvalho J E, Gilliham M, Liu L H, Obermeyer G, Feijó J A.Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine.Science, 2011, 332: 434-437 |
[21] | Konrad K R, Wudick M M, Feijó J A.Calcium regulation of tip growth: new genes for old mechanisms. Curr Opin Plant Biol, 2011, 14: 721-730 |
[22] | Dearnaley J D W, Levina N N, Lew R R, Heath B, Goring D R. Interrelationships between cytoplasmic Ca2+ peaks, pollen hydration and plasma membrane conductances during compatible and incompatible pollinations ofBrassica napus papillae. Plant Cell Physiol, 1997, 38: 985-999 |
[23] | Elleman C J, Dickinson H G.Commonalities between pollen/ stigma and host/pathogen interactions: calcium accumulation during stigmatic penetration byBrassica oleracea pollen tubes. Sex Plant Reprod, 1999, 12: 194-202 |
[24] | Goring D R.The search for components of the self-incompatibility signalling pathway (s) inBrassica napus. Ann Bot, 2000, 85(suppl-1): 171-179 |
[25] | Franklin-Tong V E, Hackett G, Hepler P K. Ratio-imaging of Ca2+ in the self-incompatibility response in pollen tubes ofPapaver rhoeas. Plant J, 1997, 12: 1375-1386 |
[26] | Wheeler M J, De Graaf B H J, Hadjiosif N, Perry R M, Poulter N S, Osman K, Vatovec S, Harper A, Franklin F C H, Franklin-Tong V E. Identification of the pollen self-incompatibility determinant inPapaver rhoeas. Nature, 2009, 459: 992-995 |
[27] | Iwano M, Ito K, Fujii S, Kakita M, Asano-Shimosato H, Lgarashi M, Kaothien-Nakayama P, Entani T, Kanatani A, Takshisa M, Tanaka M, Komatsu K, Shiba H, Nagai T, Miyawaki A, Isogai A, Takayama S T.Calcium signalling mediates self-incompatibility response in the Brassicaceae. Nat Plants, 2015, 1: 15128 |
[1] | 王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠. 结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因[J]. 作物学报, 2021, 47(12): 2407-2422. |
[2] | 王玉奎,张贺翠,白晓璟,廉小平,施松梅,刘倩莹,左同鸿,朱利泉. 甘蓝BoPINs家族基因的特征和表达分析[J]. 作物学报, 2019, 45(8): 1270-1278. |
[3] | 白晓璟,廉小平,王玉奎,张贺翠,刘倩莹,左同鸿,张以忠,谢琴琴,胡燈科,任雪松,曾静,罗绍兰,蒲敏,朱利泉. 甘蓝SI相关基因BoCDPK14的克隆与分析[J]. 作物学报, 2019, 45(12): 1773-1783. |
[4] | 魏大勇, 崔艺馨, 熊清, 汤青林, 梅家琴, 李加纳, 钱伟. 用全基因组关联作图和共表达网络分析鉴定油菜种子硫苷含量的候选基因[J]. 作物学报, 2018, 44(05): 629-641. |
[5] | 黄启秀,曲延英,姚正培,李梦雨,陈全家*. 海岛棉枯萎病抗性与类黄酮代谢相关基因表达量的相关[J]. 作物学报, 2017, 43(12): 1791-1801. |
[6] | 许俊强,孙梓健,刘智宇,杨朴丽,汤青林,王志敏,宋明,王小佳. 结球甘蓝雌蕊调控因子SPT与HEC1的克隆及相互作用分析[J]. 作物学报, 2014, 40(06): 1011-1019. |
[7] | 宋明,许俊强,孙梓健,汤青林,王志敏,王小佳. 结球甘蓝花粉类钙调素蛋白基因BoCML49的克隆及表达分析[J]. 作物学报, 2012, 38(12): 2162-2169. |
|