作物学报 ›› 2024, Vol. 50 ›› Issue (1): 16-31.doi: 10.3724/SP.J.1006.2024.31013
李俣佳1, 许豪1, 于士男1, 唐建卫1, 李巧云1, 高艳1, 郑继周2, 董纯豪1, 袁雨豪1, 郑天存2, 殷贵鸿1,*()
LI Yu-Jia1, XU Hao1, YU Shi-Nan1, TANG Jian-Wei1, LI Qiao-Yun1, GAO Yan1, ZHENG Ji-Zhou2, DONG Chun-Hao1, YUAN Yu-Hao1, ZHENG Tian-Cun2, YIN Gui-Hong1,*()
摘要:
周8425B是黄淮海麦区应用较广泛的矮秆大穗、抗病抗逆小麦骨干亲本, 解析周8425B衍生品种的条锈病抗性遗传表现及其携带的抗条锈病基因遗传传递信息对新品种选用具有重要价值。本研究以条锈病强毒力生理小种条中34 (CYR34)单一菌种对收集的222份周8425B衍生品种进行苗期条锈病抗性鉴定, 以CYR34为主的混合菌种对衍生品种进行成株期条锈病抗性鉴定, 利用周8425B携带的抗条锈病基因YrZH84、YrZH84.2、Yr30、YrZH22和Yr9紧密连锁的分子标记对衍生品种进行基因型检测。研究结果表明, 周8425B苗期和成株期对目前强毒力优势菌种CYR34均表现高抗条锈病。在222份周8425B的衍生品种中, 2年表现稳定成株期抗病的衍生品种有昌麦9号、济研麦10、百农4199、赛德麦7号和郑麦103等14份, 占比6.3%; 表现稳定全生育期抗病的衍生品种有周麦11、周麦22、周麦26、周麦36、兰天36、存麦16和郑品麦8号等52份, 占比23.4%。周8425B衍生品种主要通过周麦11、周麦12、周麦13、周麦15、周麦16和周麦17等6个子一代再次衍生到子二代。子一代中周麦16和周麦13由于具有较好的农艺性状直接衍生出较多品种, 周麦12与周麦13培育出子二代周麦22衍生出45个子三代, 周麦11培育出矮抗58衍生出54个子三代。周8425B携带的YrZH84、YrZH84.2、YrZH22、Yr30和Yr9在衍生后代中的频率分别为34.7%、14.9%、41.9%、66.2%和67.1%。仅携带其中1个抗病基因的衍生品种以携带YrZH84的平均严重度最低, 为15.4%; 聚合2个抗病基因的衍生品种中, 以携带YrZH84+YrZH22的平均严重度最低, 为20.0%; 聚合3个抗病基因的衍生品种中, 以携带YrZH84+YrZH22+Yr9的平均严重度最低, 为17.2%; 聚合4个抗病基因的衍生品种中, 携带YrZH84+YrZH22+Yr30+Yr9的平均严重度为16.9%, 携带YrZH84.2+YrZH22+Yr30+Yr9的平均严重度为38.4%。苗期以携带全生育期抗性基因YrZH84或含有YrZH84的基因组合的衍生品种的抗病性较好。研究结果为中国小麦骨干种质周8425B的持续改良利用提供了条锈病基因信息, 鉴定出对强毒力生理小种CYR34表现抗病的衍生新种质, 为我国小麦抗条锈病遗传育种提供了参考。
[1] |
Wang N, Tang C L, Fan X, He M Y, Gan P F, Zhang S, Hu Z Y, Wang X D, Yan T, Shu W X, Yu L G, Zhao J R, He J N, Li L L, Wang J F, Huang X L, Huang L L, Zhou J M, Kang Z S. Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi. Cell, 2022, 185: 2961-2974.
doi: 10.1016/j.cell.2022.06.027 |
[2] |
Jia M, Yang L J, Zhang W, Garry R, Li J H, Yang E N, Chen L, Wang W X, Liu Y K, Tong H W, He W J, Zhang Y Q, Zhu Z W, Gao C B. Genome-wide association analysis of stripe rust resistance in modern Chinese wheat. BMC Plant Biol, 2020, 20: 491.
doi: 10.1186/s12870-020-02693-w pmid: 33109074 |
[3] | 韩德俊, 王琪琳, 张立, 魏国荣, 曾庆东, 赵杰, 王晓杰, 黄丽丽, 康振生. “西北-华北-长江中下游”条锈病流行区系当前小麦品种(系)抗条锈病性评价. 中国农业科学, 2010, 43: 2889-2896. |
Han D J, Wang Q L, Zhang L, Wei G R, Zeng Q D, Zhao J, Wang X J, Huang L L, Kang Z S. Identification and evaluation of resistance to stripe rust in 1980 wheat landraces and abroad germplasm. Sci Agric Sin, 2010, 43: 2889-2896. (in Chinese with English abstract) | |
[4] | 刘博, 刘太国, 章振羽, 贾秋珍, 王保通, 高利, 彭云良, 金社林, 陈万权. 中国小麦条锈菌条中34号的发现及其致病特性. 植物病理学报, 2017, 47: 681-687. |
Liu B, Liu T G, Zhang Z Y, Jia Q Z, Wang B T, Gao L, Peng Y L, Jin S L, Chen W Q. Discovery and pathogenicity of CYR34, a new race of Puccinia striiformis f. sp. tritici in China. Acta Phytopath Sin, 2017, 47: 681-687. (in Chinese with English abstract) | |
[5] | 黄瑾, 贾秋珍, 张勃, 孙振宇, 黄苗苗, 金社林. 小麦条锈病菌新菌系G22-9 (CYR34) 和G22-14流行趋势预测. 植物保护学报, 2018, 45: 101-108. |
Huang J, Jia Q Z, Zhang B, Sun Z Y, Jin S L. Epidemic forecasting of the new strains G22-9 (CYR34) and G22-14 of Puccinia striiformis f. sp. tritici in wheat in Gansu province. J Plant Protect, 2018, 45: 101-108. (in Chinese with English abstract) | |
[6] | 黄亮, 刘太国, 刘博, 高利, 罗培高, 陈万权. 我国197份小麦核心种质资源对小麦条锈菌新小种CYR34的抗性评价. 植物保护, 2019, 45: 148-154. |
Huang L, Liu T G, Liu B, Gao L, Luo P G, Chen W Q. Resistance evaluation of 197 Chinese wheat core germplasms to a new stripe rust race, CYR34. Plant Protect, 2019, 45: 148-154. (in Chinese with English abstract) | |
[7] | 吴舒舒. 中国小麦主产区270份新育成品种(系)条锈病抗性评价. 西北农林科技大学硕士学位论文, 陕西杨凌, 2021. |
Wu S S. Evaluation of Stripe Rust Resistance of 270 Bred Wheat Cultivars (Lines) from the Main Wheat Region in China. MS Thesis of Northwest A&F University, Yangling, Shaanxi, China, 2021. (in Chinese with English abstract) | |
[8] |
管方念, 龙黎, 姚方杰, 王昱琦, 江千涛, 康厚扬, 蒋云峰, 李伟, 邓梅, 李豪, 陈国跃. 152份黄淮海麦区小麦农家品种抗条锈性评价及重要条锈病抗性基因的分子检测. 中国农业科学, 2020, 53: 3629-3637.
doi: 10.3864/j.issn.0578-1752.2020.18.001 |
Guan F N, Long L, Yao F J, Wang Y Q, Jiang Q T, Kang H Y, Jiang Y F, Li W, Deng M, Li H, Chen G Y. Evaluation of resistance to stripe rust and molecular detection of important known Yr gene(s) of 152 Chinese wheat landraces from the Huang-Huai- Hai. Sci Agric Sin, 2020, 53: 3629-3637. (in Chinese with English abstract) | |
[9] | 胡朝月, 王凤涛, 郎晓威, 冯晶, 李俊凯, 蔺瑞明, 姚小波. 小麦抗条锈病基因对中国条锈菌主要流行小种的抗性分析. 中国农业科学, 2022, 55: 491-502. |
Hu C Y, Wang F T, Lang X W, Feng J, Li J K, Lin R M, Yao X B. Resistance analyses on wheat stripe rust resistance genes to the predominant races of Puccinia striiformis f. sp. tritici in China. Sci Agric Sin, 2022, 55: 491-502 (in Chinese with English abstract). | |
[10] |
Wu J, Xu D, Fu L, Wu L, Hao W H, Li J H, Dong Y, Wang F J, Wu Y Y, He Z H, Si H Q, Ma C X, Xia X C. Fine mapping of a stripe rust resistance gene YrZM175 in bread wheat. Theor Appl Genet, 2022, 135: 3485-3496.
doi: 10.1007/s00122-022-04195-9 |
[11] |
Zhou X, Fang T, Li K, Huang K B, Ma C H, Zhang M, Li X, Yang S Z, Ren R S, Zhang P P. Yield losses associated with different levels of stripe rust resistance of commercial wheat cultivars in China. Phytopathology, 2022, 112: 1244-1254.
doi: 10.1094/PHYTO-07-21-0286-R |
[12] | 张德强, 宋晓朋, 冯洁, 连俊芳, 孙道杰. 小麦周8425B及其衍生品种与黄淮麦区主栽品种的遗传解析. 麦类作物学报, 2016, 36: 1328-1334. |
Zhang D Q, Song X P, Feng J, Lian J F, Sun D J. Genetic analysis of wheat Zhou 8425B and its derivatives and main varieties in Huang-Huai wheat area. J Triticeae Crops, 2016, 36: 1328-1334. (in Chinese with English abstract) | |
[13] |
肖永贵, 殷贵鸿, 李慧慧, 夏先春, 阎俊, 郑天存, 吉万全, 何中虎. 小麦骨干亲本“周 8425B”及其衍生品种的遗传解析和抗条锈病基因定位. 中国农业科学, 2011, 44: 3919-3929.
doi: 10.3864/j.issn.0578-1752.2011.19.001 |
Xiao Y G, Yin G H, Li H H, Xia X C, Yan J, Zheng T C, Ji W Q, Wu Z H. Genetic diversity and genome-wide association analysis of stripe rust resistance among the core wheat parent Zhou 8425B and its derivatives. Sci Agric Sin, 2011, 44: 3919-3929. (in Chinese with English abstract) | |
[14] | 邹少奎, 殷贵鸿, 唐建卫, 王俊生, 韩玉林, 李顺成, 李楠楠. 黄淮主推小麦品种主要农艺性状配合力及遗传效应分析. 麦类作物学报, 2017, 37: 730-738. |
Zou S K, Yin G H, Tang J W, Wang J S, Han Y L, Li S C, Li N N. Combining ability and genetic effect analysis of main agronomic traits of cultivars in Huang-Huai wheat region. J Triticeae Crops, 2017, 37: 730-738. (in Chinese with English abstract) | |
[15] |
唐建卫, 殷贵鸿, 王丽娜, 韩玉林, 黄峰, 于海飞, 杨光宇, 李新平. 小麦湿面筋含量和面筋指数遗传分析. 作物学报, 2011, 37: 1701-1706.
doi: 10.3724/SP.J.1006.2011.01701 |
Tang J W, Yin G H, Wang L N, Han Y L, Huang F, Yu H F, Yang G Y, Li X P. Inheritance of wet gluten content and gluten index in wheat. Acta Agron Sin, 2011, 37: 1701-1706. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2011.01701 |
|
[16] |
唐建卫, 殷贵鸿, 韩玉林, 王丽娜, 于海飞, 杨光宇, 李新平. 周麦16号主要农艺性状配合力及遗传效应分析. 河南农业科学, 2010, (11): 14-18.
doi: 10.3969/j.issn.1004-3268.2010.11.003 |
Tang J W, Yin G H, Han Y L, Wang L N, Yu H F, Yang G Y, Li X P. Analysis of combining ability and genetic effect of main agronomic characters of Zhoumai 16. J Henan Agric Sci, 2010, (11):14-18. (in Chinese with English abstract) | |
[17] | 邹少奎, 殷贵鸿, 唐建卫, 韩玉林, 李顺成, 李楠楠, 黄峰, 王丽娜, 张倩, 高艳. 小麦品种周麦22号的分子遗传基础及其特异引物筛选. 麦类作物学报, 2017, 37: 472-482. |
Zou S K, Yin G H, Tang J W, Han Y L, Li S C, Li N N, Huang F, Wang L N, Zhang Q, Gao Y. Molecular and genetic basis of wheat variety Zhoumai 22 and specific primers screening. J Triticeae Crops, 2017, 37: 472-482 (in Chinese with English abstract). | |
[18] | 唐建卫, 高艳, 胡润雨, 张根源, 殷贵鸿, 耿洪伟. 近十年基于重要小麦亲本周麦22的遗传改良. 麦类作物学报, 2021, 41: 263-271. |
Tang J W, Gao Y, Hu R Y, Zhang G Y, Yin G H, Geng H W. Genetic improvement based on the important wheat parent Zhoumai 22 in recent ten years. J Triticeae Crops, 2021, 41: 263-271. (in Chinese with English abstract) | |
[19] |
高艳, 唐建卫, 邹少奎, 胡润雨, 张根源, 孙玉霞, 王磊, 殷贵鸿. 小麦周麦22及其衍生品种的遗传多样性分析. 植物遗传资源学报, 2021, 22: 38-49.
doi: 10.13430/j.cnki.jpgr.20200419002 |
Gao Y, Tang J W, Zou S K, Hu R Y, Zhang G Y, Sun Y X, Wang L, Yin G H. Genetic diversity assessment on derivatives from wheat cultivar Zhoumai 22. J Plant Genet Resour, 2021, 22: 38-49. (in Chinese with English abstract) | |
[20] | 刘志勇, 张怀志, 白斌, 李俊, 黄林, 徐智斌, 陈永兴, 刘旭, 曹廷杰, 李淼淼, 陆平, 吴秋红, 董玲丽, 韩玉林, 殷贵鸿, 胡卫国, 王西成, 赵虹, 闫素红, 杨兆生, 畅志坚, 王涛, 杨武云, 刘登才, 李洪杰, 杜久元. 中国小麦抗条锈病基因育种利用现状与策略. 中国农业科学, (2022-09-29). https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.220610. |
Liu Z Y, Zhang H Z, Bai B, Li J, Huang L, Xu Z B, Chen Y X, Liu X, Cao T Y, Li N N, Lu P, Wu Q H, Dong L L, Han Y L, Yin G H, Hu W G, Wang X C, Zhao H, Yan S H, Yang Z S, Chang Z J, Wang T, Yang W Y, Liu D C, Li H J, Du J Y. Latest employment: current status and strategies for utilization of stripe rust resistance genes in wheat breeding program of China. Sci Agric Sin, (2022-09-29). https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.220610. (in Chinese with English abstract) (in press). | |
[21] | Li Z F, Zheng T C, He Z H, Li G Q, Xu S C, Li X P, Yang G Y, Singh R P, Xia X C. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet, 2006, 112: 1198-1103. |
[22] | 任妍.普通小麦抗条锈病基因分子定位. 中国农业科学院博士学位论文, 北京, 2012. |
Ren Y.Molecular Mapping of Stripe Rust Resistance Gene in Common Wheat. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2012. (in Chinese with English abstract) | |
[23] |
Wang Y, Xie J Z, Zhang H Z, Guo B M, Ning S Z, Chen Y X, Lu P, Wu Q H, Li M M, Zhang D Y, Guo G H, Zhang Y, Liu D C, Zou S K, Tang J W, Zhao H, Wang X C, Li J, Yang W Y, Cao T J, Yin G H, Liu Z Y. Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses. Theor Appl Genet, 2017, 130: 2191-2201.
doi: 10.1007/s00122-017-2950-0 |
[24] |
Liu S J, Wang X T, Zhang Y, Jin Y G, Xia Z H, Xiang M J, Huang S, Qiao L Y, Zheng W J, Zeng Q D, Wang Q L, Yu R, Singh R P, Bhavani S, Kang Z S, Han D J, Wang C F, Wu J H. Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL. Theor Appl Genet, 2022, 135: 351-365.
doi: 10.1007/s00122-021-03970-4 |
[25] | 阳霞, 简俊涛, 朱传杰, 张玲丽. 小麦抗病基因Lr68和Sr2/Yr30的遗传特性分析. 麦类作物学报, 2014, 34: 151-156. |
Yang X, Jian J T, Zhu C J, Zhang L L. Genetic characteristics of wheat disease resistance genes Lr68 and Sr2/Yr30. J Triticeae Crops, 2014, 34: 151-156. (in Chinese with English abstract) | |
[26] |
Hayden M J, Kuchel H, Chalmers K J. Sequence tagged microsatellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1641-1647.
pmid: 15340687 |
[27] |
Crossa J, Burgueño J, Dreisigacker S, Vargas M, Herrera-Foessel A S, Lillemo M, Singh R P, Trethowan R, Warburton M, Franco J, Reynolds M, Crouch H J, Ortiz R. Association analysis of historical bread wheat germplasm using additive genetic covariance of relatives and population structure. Genetics, 2007, 177: 1889-1913.
doi: 10.1534/genetics.107.078659 pmid: 17947425 |
[28] |
路子峰, 时晓磊, 李玉姗, 崔燕华, 仙鹤, 严勇亮, 张金波, 高海峰, 丛花. 153份新疆冬小麦品种3个条锈病抗性基因的分子检测. 新疆农业科学, 2022, 59: 2924-2932.
doi: 10.6048/j.issn.1001-4330.2022.12.007 |
Lu Z F, Shi X L, Li Y S, Cui Y H, Xian H, Yan Y L, Zhang J B, Gao H F, Cong H. Molecular detection of three Yr genes in 153 winter wheat varieties from Xinjiang. Xinjiang Agric Sci, 2022, 59: 2924-2932. | |
[29] | 张晓祥, 王玲, 寿路路. 一种快速提取小麦基因组DNA的改良CTAB方法. 中国农学通报, 2012, 28(36): 46-49. |
Zhang X L, Wang L, Shou L L. A rapid modified CTAB method of extracting genomic DNA from wheat leaf. Agric Sci Tech, 2012, 28(36): 46-49. (in Chinese with English abstract) | |
[30] | Peterson R F, Campbell A B, Hannah A E. A diagrammatic scale for estimating rust intensity on leaves and leaves and stems of cereals. Can J Biochem Physiol, 1948, 26: 496-500. |
[31] | 焦韩轩, 孙晓媛, 黄硕, 刘胜杰, 秦华, 吴建辉, 曾庆东, 王长发, 李海峰, 康振生, 韩德俊. 土耳其小麦种质条锈病抗性评价和抗病基因分析. 麦类作物学报, 2021, 41: 799-805. |
Jiao H X, Sun X Y, Huang S, Liu S J, Qin H, Wu J H, Zeng Q D, Wang C F, Li H F, Kang Z S, Han D J. Evaluation of resistance to stripe rust and gene analysis in Turkish wheat germplasms. J Triticeae Crops, 2021, 41: 799-805 (in Chinese with English abstract). | |
[32] |
Pang Y L, Liu C X, Lin M, Ni F, Li W H, Cai J, Zhang Z L, Zhu H Q, Liu J X, Wu J J, Bai G H, Liu S B. Mapping QTL for adult-plant resistance to stripe rust in a Chinese wheat landrace. Int J Mol Sci, 2022, 23: 9662-9663.
doi: 10.3390/ijms23179662 |
[33] |
Yang Q, Yang D, Li P, Liang S, Zhang Z. A bibliometric and visual analysis of global community resilience research. Int J Environ Res Public Health, 2021, 18: 10857.
doi: 10.3390/ijerph182010857 |
[34] |
Shen G X, Wang W L. Circlize package in R and analytic hierarchy process (AHP): contribution values of ABCDE and AGL6 genes in the context of floral organ development. PLoS One, 2022, 17: e0261232.
doi: 10.1371/journal.pone.0261232 |
[35] | 刘万才, 王保通, 赵中华, 李跃, 康振生. 我国小麦条锈病历次大流行的历史回顾与对策建议. 中国植保导刊, 2022, 42(6): 21-27. |
Liu W C, Wang B T, Zhao Z H, Li Y, Kang Z S. Historical review and countermeasures of wheat stripe rust epidemics in China. China Plant Prot, 2022, 42(6): 21-27. (in Chinese with English abstract) | |
[36] |
何中虎, 夏先春, 陈新民, 庄巧生. 中国小麦育种进展与展望. 作物学报, 2011, 37: 202-215.
doi: 10.3724/SP.J.1006.2011.00202 |
He Z H, Xia X C, Chen X M, Zhuang Q S. Progress of wheat breeding in China and the future perspective. Acta Agron Sin, 2011, 37: 202-215. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2011.00202 |
|
[37] |
Liu D, Yuan C, Singh R P, Randhawa M S, Sridhar B, Kumar U, Huerta-Espino J, Lagudah E, Lan C X. Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci. Front Plant Sci, 2022, 13: 880138.
doi: 10.3389/fpls.2022.880138 |
[1] | 习玲, 王昱琦, 朱微, 王益, 陈国跃, 蒲宗君, 周永红, 康厚扬. 78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测[J]. 作物学报, 2021, 47(7): 1309-1323. |
[2] | 赵旭阳, 姚方杰, 龙黎, 王昱琦, 康厚扬, 蒋云峰, 李伟, 邓梅, 李豪, 陈国跃. 青藏春冬麦区93份小麦地方种质条锈病抗性评价及抗病基因分子鉴定[J]. 作物学报, 2021, 47(10): 2053-2063. |
[3] | 白宗璠,竞霞,张腾,董莹莹. MDBPSO算法优化的全波段光谱数据协同冠层SIF监测小麦条锈病[J]. 作物学报, 2020, 46(8): 1248-1257. |
[4] | 刘忠祥,杨梅,殷鹏程,周玉乾,何海军,邱法展. 玉米株高主效QTL qPH3.2精细定位及遗传效应分析[J]. 作物学报, 2018, 44(9): 1357-1366. |
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