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作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1338-1346.doi: 10.3724/SP.J.1006.2025.42048

• 耕作栽培·生理生化 • 上一篇    

安徽省不同生理小种稻瘟病菌对稻瘟灵的敏感性研究

沈文杰1，陈晴晴1，胡逸群1，张爱芳1,*，张曼玉2   

  1. 1 安徽省农业科学院植物保护与农产品质量安全研究所 / 粮食和蔬菜病虫害抗药性治理安徽省重点实验室, 安徽合肥230001; 2 宿州市农业科学院, 安徽宿州234099
  • 收稿日期:2024-10-28 修回日期:2025-01-23 接受日期:2025-01-23 出版日期:2025-05-12 网络出版日期:2025-02-11
  • 基金资助:
    本研究由安徽省农业财政资助项目(安徽省水稻品种区域试验2021–2023)资助。

Study on the sensitivity of various physiological races of Magnaporthe oryzae to isoprothiolane in Anhui province

SHEN Wen-Jie1,CHEN Qing-Qing1,HU Yi-Qun1,ZHANG Ai-Fang1,*,ZHANG Man-Yu2   

  1. 1 Institute of Plant Protection and Agricultural Product Quality Safety, Anhui Academy of Agricultural Sciences / Anhui Province Key Laboratory of Pesticide Resistance Management on Grain and Vegetable Pests, Hefei 230001, Anhui, China; 2 Suzhou Academy of Agricultural Sciences, Suzhou 234099, Anhui, China
  • Received:2024-10-28 Revised:2025-01-23 Accepted:2025-01-23 Published:2025-05-12 Published online:2025-02-11
  • Supported by:
    This study was supported by the Anhui Provincial Agricultural Finance Support Project (Anhui Province Rice Variety Regional Trial 2021–2023).

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摘要：

为探究安徽省不同生理小种的稻瘟病菌对稻瘟灵的敏感性差异，本研究于2021—2022年在安徽省17个地区采集分离173株稻瘟病菌，利用中国7个鉴别寄主对这173株稻瘟病菌进行生理小种鉴定，采用菌丝生长速率法测定稻瘟病菌对稻瘟灵的敏感性及该药与其他药剂的交互抗性。试验结果表明：供试稻瘟病菌菌株可划分为ZA、ZB、ZC、ZG和ZH共5个种群，其中优势种群为ZB种群，优势生理小种为ZB13。供试菌株抑制中浓度EC50值范围为1.68~28.83 μg mL?1，本研究将5.54 μg mL?1定为安徽省稻瘟病菌对稻瘟灵的敏感性基线，划分出敏感菌株102株，低抗菌株70株，中抗菌株1株。在凤台县、宣州区等地鉴定到的ZB13、ZB15、ZB16等优势生理小种抗性水平明显低于南陵县、石台县等地，说明安徽省稻瘟病菌对稻瘟灵敏感性差异主要与地区有关，与生理小种无明显相关性。供试菌株对稻瘟灵的敏感性与对吡唑醚菌酯、咪鲜胺的敏感性之间没有显著相关性。在合理轮换使用不同杀菌剂的前提下，稻瘟灵仍然可以作为安徽省防治稻瘟病的主要杀菌剂。

关键词: 安徽省, 稻瘟病菌, 生理小种, 稻瘟灵, 抗药性

Abstract:

To investigate variations in the sensitivity of different physiological races of Magnaporthe oryzae to isoprothiolane in Anhui Province, 173 M. oryzae isolates were collected from 17 regions across the province during 2021–2022. The physiological races of these isolates were identified using seven differential rice hosts from China. The sensitivity of M. oryzae to isoprothiolane, along with potential cross-resistance to other fungicides, was assessed by measuring mycelial growth rates. The results showed that the 173 M. oryzae isolates belonged to five populations: ZA, ZB, ZC, ZG, and ZH. Among these, the ZB population was predominant, with ZB13 identified as the dominant physiological race. The EC50 values of the isolates ranged from 1.68 to 28.83 μg mL?1, and a baseline sensitivity value of 5.54 μg mL?1 was established for isoprothiolane in Anhui province. Among the isolates, 102 were classified as sensitive, 70 as low-resistant, and 1 as moderately resistant. The resistance levels of the dominant physiological races ZB13, ZB15, and ZB16, identified in regions such as Fengtai County and Xuancheng District, were significantly lower than those observed in regions such as Nanling County and Shitai County. This finding suggests that variations in the sensitivity of M. oryzae to isoprothiolane in Anhui Province are primarily influenced by geographic location rather than physiological race. Furthermore, no significant correlation was found between sensitivity to isoprothiolane and sensitivity to pyraclostrobin or prochloraz. These results indicate that, under the premise of rational rotation with other fungicides, isoprothiolane can continue to serve as a key fungicide for managing rice blast disease in Anhui province.

Key words: Anhui Province, Magnaporthe oryzae, physiological races, isoprothiolane, resistance

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