作物学报 ›› 2022, Vol. 48 ›› Issue (10): 2443-2450.doi: 10.3724/SP.J.1006.2022.14188
练云(), 魏荷, 王金社, 张辉, 雷晨芳, 李金英, 卢为国()
LIAN Yun(), WEI He, WANG Jin-She, ZHANG Hui, LEI Chen-Fang, LI Jin-Ying, LU Wei-Guo()
摘要:
由大豆胞囊线虫(Soybean cyst nematode, Heterodera glycines, SCN)引起的病害是一种世界性大豆病害。随着强致病力大豆胞囊线虫群体X12的出现及LY1线虫群体的合成, 国际通用的Riggs和HG type 2种大豆胞囊线虫生理小种鉴别模式已不能将4号生理小种、X12线虫群体、LY1线虫群体有效区分, 本研究提供了一种区分这3个线虫群体的简易鉴定方法, 为SCN相关研究提供技术支撑。包括以下步骤: 利用感病品种在病土中繁殖备用接种的胞囊; 用兴县灰皮支(ZDD2315)和PI567516C作为鉴别寄主, Lee为感病对照, 接种鉴定; 若兴县灰皮支和PI567516C均表现感病, 表明该病土感染的是X12线虫群体; 若兴县灰皮支表现抗病而PI567516C表现感病, 表明该病土感染的是4号生理小种; 若PI567516C表现抗病, 表明该病土感染的是LY1线虫群体。以上结果表明, 利用我国优异抗源兴县灰皮支和PI567516C 作为鉴别寄主, 能有效区分目前报道的具有强致病力的大豆胞囊线虫4号生理小种、X12和LY1线虫群体。本研究结果对筛选抗源、调查大豆胞囊线虫生理小种分布、线虫致病基因研究有重要意义。
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