作物学报 ›› 2017, Vol. 43 ›› Issue (06): 795-810.doi: 10.3724/SP.J.1006.2017.00795
李旭升1,**,向小娇2,**,申聪聪2,杨隆维1,*,陈凯2,3,王小文1,邱先进1,朱小源4,邢丹英1,徐建龙2,3,*
LI Xu-Sheng1,**,XIANG Xiao-Jiao2,**,SHEN Cong-Cong2,YANG Long-Wei1,*,CHEN Kai3,WANG Xiao-Wen1,QIU Xian-Jin1,ZHU Xiao-Yuan4,XING Dan-Ying1,XU Jian-Long2,3,*
摘要:
稻瘟病一直是制约水稻产量的重要因素之一,稻瘟病抗源筛选是抗性基因挖掘和抗病育种的基础。本试验利用3000份(简称3K)重测序中的1217份核心种质资源,通过湖北恩施两河和芭蕉两个病圃自然诱发鉴定抗性,结合不发病条件下农艺性状考察和抗病资源的苗期人工接种抗谱测定,综合评价和筛选优异的稻瘟病抗源。自然诱发鉴定结果显示材料间的稻瘟病抗感差异显著,从中共获得144份抗苗瘟、叶瘟和穗瘟的抗病种质。选用稻瘟病综合抗性较好的34份材料以30个不同来源的稻瘟病菌株苗期接种,鉴定显示有17份材料的抗性频率≥70%,抗谱较广。农艺性状考察结果显示,大部分抗病材料植株偏高,单株产量低,农艺性状差。结合病圃鉴定、人工接种鉴定和农艺性状考察,鉴定出IRGA 411-1-6-1F-A、YJ30、金早47、泉珍10号、YN 1353-3、云粳23和IRAT 1047等7份稻瘟病抗性强、抗谱广且农艺性状较好的优异抗源材料,可作为抗源亲本用于稻瘟病抗性基因挖掘和品种抗稻瘟性改良。
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