作物学报 ›› 2017, Vol. 43 ›› Issue (06): 811-820.doi: 10.3724/SP.J.1006.2017.00811
邹雪1,2,邓孟胜2,李立芹2,余金龙1,丁凡1,黄雪丽2,彭洁2,帅禹2,蔡诚诚2,王西瑶2,*
ZOU Xue1,2,DENG Meng-Sheng2,LI Li-Qin2,YU Jin-Long1,DING Fan1,HUANG Xue-Li2,PENG Jie2,SHUAI Yu2,CAI Cheng-Cheng2,WANG Xi-Yao2,*
摘要:
为探明油菜素内酯BR在块茎萌芽中的作用,建立更有效的种薯催芽调控体系,选择了休眠期不同的3个品种,利用qRT-PCR分析与BR合成、信号转导、调控有关的9个基因在贮藏期间及抑芽处理下的表达模式;同时检测BR类似物24-表油菜素内酯(24-eBL)及其与赤霉素GA3对块茎萌芽的影响。结果表明,涉及BR合成的4个基因表达量均随贮藏时间延长升高,短休眠品种升高的时间点早于中、长休眠品种;信号转导及调控基因中BRI1和CYCD3的变化与合成基因相似,BSK和TCH4的表达量则在中、长休眠期品种中保持恒定。抑芽处理在贮藏前期能刺激这些基因的表达升高,但之后都迅速下降并保持低水平。转录因子BZR1在各品种中以及抑芽处理下均没有明显变化。24-eBL利于块茎解除休眠,但不促进芽的伸长生长,与GA3互配效果更佳,单株块茎增重37.92%~98.41%。结论表明,BR合成和信号转导是块茎从休眠向萌芽转变的必经生理过程,它与GA3互配用于催芽更利于种薯萌芽的整齐、健壮并促进块茎形成。
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