作物学报 ›› 2017, Vol. 43 ›› Issue (06): 811-820.doi: 10.3724/SP.J.1006.2017.00811
邹雪1,2,邓孟胜2,李立芹2,余金龙1,丁凡1,黄雪丽2,彭洁2,帅禹2,蔡诚诚2,王西瑶2,*
ZOU Xue1,2,DENG Meng-Sheng2,LI Li-Qin2,YU Jin-Long1,DING Fan1,HUANG Xue-Li2,PENG Jie2,SHUAI Yu2,CAI Cheng-Cheng2,WANG Xi-Yao2,*
摘要:
为探明油菜素内酯BR在块茎萌芽中的作用,建立更有效的种薯催芽调控体系,选择了休眠期不同的3个品种,利用qRT-PCR分析与BR合成、信号转导、调控有关的9个基因在贮藏期间及抑芽处理下的表达模式;同时检测BR类似物24-表油菜素内酯(24-eBL)及其与赤霉素GA3对块茎萌芽的影响。结果表明,涉及BR合成的4个基因表达量均随贮藏时间延长升高,短休眠品种升高的时间点早于中、长休眠品种;信号转导及调控基因中BRI1和CYCD3的变化与合成基因相似,BSK和TCH4的表达量则在中、长休眠期品种中保持恒定。抑芽处理在贮藏前期能刺激这些基因的表达升高,但之后都迅速下降并保持低水平。转录因子BZR1在各品种中以及抑芽处理下均没有明显变化。24-eBL利于块茎解除休眠,但不促进芽的伸长生长,与GA3互配效果更佳,单株块茎增重37.92%~98.41%。结论表明,BR合成和信号转导是块茎从休眠向萌芽转变的必经生理过程,它与GA3互配用于催芽更利于种薯萌芽的整齐、健壮并促进块茎形成。
| [1] Bamberg J B. Tuber dormancy lasting eight years in the wild potato Solanum jamesii. Am J Potato Res, 2010, 87: 226–228 [2] 樊荣, 宋波涛, 谢从华, 柳俊. 春秋两季马铃薯微型薯休眠期及发芽特性比较分析. 中国马铃薯, 2009, 23(5): 277–280 Fan R, Song P T, Xie C H, Liu J. Comparison of dormancy and sprouting between potato minitubers produced in spring and autumn seasons. Chin Potato J, 2009, 23(5): 277–280 (in Chinese with English abstract) [3] 肖关丽, 郭华春. 不同生理年龄马铃薯种薯芽中的内源激素含量变化及其对马铃薯植株生长发育的影响. 植物生理学报, 2007, 43: 818–820 Xiao G L, Guo H C. Changes in endogenous hormone contents in bud of seed potato (Solanum tuberosum L.) with different physiological ages and its effect on growth and development. Plant Physiol J, 2007, 43: 818–820 (in Chinese with English abstract) [4] Aksenova N P, Sergeeva L I, Konstantinova T N, Golyanovskaya S A, Kolachevskaya O O, Romanov G A. Regulation of potato tuber dormancy and sprouting. Russian J Plant Physiol, 2013, 60: 301–312. [5] Hartmann A, Senning M, Hedden P, Sonnewald U, Sonnewald S. Reactivation of meristem activity and sprout growth in potato tubers require both cytokinin and gibberellin. Plant Physiol, 2011, 155: 776–779 [6] Korableva N P, Platonova T A, Dogonadze M Z, Evaunina A S. Brassinolide effect on growth of apical meristems, ethylene production, and abscisic acid content in potato tubers. Biol Plant, 2002, 45: 39–43 [7] Clouse S D, Sasse J M. BRASSINOSTEROIDS: essential regulators of plant growth and development. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 427–451 [8] Steber C M, Mccourt P. A Role for Brassinosteroids in germination in Arabidopsis. Plant Physiol, 2001, 125: 763–769 [9] 董登峰, 李杨瑞, 江立庚. 油菜素内酯对铝胁迫大豆光合特性的影响. 作物学报, 2008, 34: 1673–1678 Dong D F, Li Y R, Jiang L G. Effects of brassinosteroid on photosynthetic characteristics in soybean under aluminum stress. Acta Agron Sin, 2008, 34: 1673–1678 (in Chinese with English abstract) [10] Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier C P, Gregory B D, Ross A S, Tanaka A, Yoshida S, Tax F E, Feldmann K A. The Arabidopsis dwf 7/ste1 mutant is defective in the Δ7 sterol C-5 desaturation dtep leading to brassinosteroid biosynthesis. Plant Cell, 1999, 11: 207–221 [11] Choe S, Dilkes B P, Gregory B D, Ross A S, Yuan H, Noguchi T, Fujioka S, Takatsuto S, Tanaka A, Yoshida S, Tax F E, Feldmann K A. The Arabidopsis dwarf1 mutant is defective in the conversion of 24-methylenecholesterol to campesterol in brassinosteroid biosynthesis. Plant Physiol, 1999, 119: 897–907 [12] Mi K, Fujioka S, Ji H J, Kim H B, Takatsuto S T. A double mutant for the CYP85A1 and CYP85A2 genes of Arabidopsis exhibits a brassinosteroid dwarf phenotype. J Plant Biol, 2005, 48: 237–244 [13] Clouse S D. Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development. Plant Cell, 2011, 23: 1219–1230 [14] Tang W Q, Kim T W, Oses-Prieto J A, Sun Y, Deng Z P, Zhu S W, Wang R J, Burlingame A L, Wang Z Y. BSKs mediate signal transduction from the receptor kinase BRI1 in Arabidopsis. Science, 2008, 321: 557–560 [15] Zhang C, Xu Y Y, Guo S Y, Zhu J Y, Huan Q, Liu H H, Wang L, Luo G Z, Wang X J, Chong K. Dynamics of brassinosteroid response modulated by negative regulator LIC in rice. PloS Genet, 2012, 8: e1002686 [16] Hu Y, Bao F, Li J. Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Plant J, 2000, 24: 693–701 [17] Xu W, Purugganan M M, Polisensky D H, Antosiewicz D M, Fry S C, Braam J. Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. Plant Cell,1995, 7: 1555–1567 [18] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods, 2001, 25: 402–408 [19] Gachotte D, Meens R, Benveniste P. An Arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by ERG 3 encoding a Δ7-sterol-C-5-desaturase from yeast. Plant J, 1995, 8: 407–416 [20] Iliev E A, Xu W, Polisensky D H, Oh M H, Torisky R S, Clouse S D, Braam J. Transcriptional and posttranscriptional regulation of Arabidopsis TCH4 expression by diverse stimuli: roles of cis regions and brassinosteroids. Plant Physiol, 2002, 130: 770–783 [21] Divi U K, Krishna P. Overexpression of the brassinosteroid biosynthetic gene AtDWF4 in Arabidopsis seeds overcomes abscisic acid-induced inhibition of germination and increases cold tolerance in transgenic seedlings. Plant Growth Regul, 2010, 29: 385–393 [22] Ahammed G J, Zhang S, Shi K, Zhou Y H, Yu J Q. Brassinosteroid improves seed germination and early development of tomato seedling under phenanthrene stress. Plant Growth Regul, 2012, 68: 87–96 [23] Hu Y R, Yu D Q. BRASSINOSTEROID INSENSITIVE2 interacts with ABSCISIC ACID INSENSITIVE5 to mediate the antagonism of brassinosteroids to abscisic acid during seed germination in Arabidopsis. Plant Cell, 2014, 26: 4393–4408 [24] Bai M Y, Shang J X, Oh E, Fan M, Bai Y, Zentella R, Sun T P, Wang Z Y. Brassinosteroid, gibberellins and phytochrome impinge on a common transcription module in Arabidopsis. Nat Cell Biol, 2012, 14: 810–816 [25] Gallego-Bartolomé J, Minguet E G, Grau-Enguix F, Abbas M, Locascio A, Thomas S G, Alabadí D, Blázquez M A. Molecular mechanism for the interaction between gibberellins and brassinosteroid signaling pathways in Arabidopsis. Proc Natl Acad Sci USA, 2012, 109: 13446–13451 [26] Tong H N, Xiao Y H, Liu D P, Gao S P, Liu L C, Yin Y H, Jin Y, Qian Q, Chu C C. Brassinosteroid regulates cell elongation by modulating gibberellins metabolism in rice. Plant Cell, 2014, 26: 4376–4393 [27] 张海丽, 高静, 张昊, 李生辉, 邢继红, 王凤茹, 董金皋. 油菜素内酯对水稻细胞伸长和分裂的调控. 农业生物技术学报, 2015, 23: 71–79 Zhang H L, Gao J, Zhang H, Li S H, Xing J H, Wang F R, Dong J G. The regulation of brassinosteroid (BR) on elongation and division of rice (Oryza sativa) cells. J Agric Biotechnol, 2015, 23: 71–79 (in Chinese with English abstract) [28] Zullo M A T, Adam G. Brassinosteroid phytohormones-structure, bioactivity and applications. Braz J Plant Physiol, 2002, 14: 143–181 [29] 刘海英, 郭天财, 朱云集, 王晨阳, 康国章. 开花期外施表油菜素内酯(epi-BR)对小麦籽粒淀粉积累及其关键酶活性的影响. 作物学报, 2006, 32: 924–930 Liu H Y, Guo T C, Zhu Y J, Wang C Y, Kang G Z. Effects of epi-brassinolide (epi-BR) application at anthesis on starch accumulation and activities of key enzymes in wheat grains. Acta Agron Sin, 2006, 32: 924–930 (in Chinese with English abstract) [30] Divi U K, Krishna P. Brassinosteroid: a biotechnological target for enhancing crop yield and stress tolerance. New Biotechnol, 2009, 26: 131–136 [31] Wu C Y, Trieu A, Radhakrishnan P, Kwok S F, Harris S, Zhang K, Wang J, Wan J, Zhai H, Takatsuto S, Matsumoto S, Fujioka S, Feldmann K A, Pennell R I. Brassinosteroids regulate grain filling in rice. Plant Cell, 2008, 20: 2130–2145 [32] Schr?der F, Lisso J, Obata T, Erban A, Maximova E, Giavalisco P, Kopka J, Fernie A R, Willmitzer L, Müssig C. Consequences of induced brassinosteroid deficiency in Arabidopsis leaves. BMC Plant Biol, 2014, 14: 309–322 |
| [1] | 陈国欢, 张锐, 李艳迪, 赵佳琪, 任湧涛, 张天赐, 郭华春, 李俊, 杨芳. 外源硒叶面喷施对浅紫色马铃薯块茎花青素合成的影响[J]. 作物学报, 2026, 52(6): 1876-1890. |
| [2] | 王文辕, 燕雪嘉, 刘玉霖, 孙晓彤, 李亚楠, 唐鑫华, 石瑛. 耐弱光马铃薯品种筛选及转录因子编码基因StPIF3的克隆与功能分析[J]. 作物学报, 2026, 52(6): 1631-1645. |
| [3] | 田立涛, 丁宁, 王树林, 齐恩芳, 张荣, 王蕊蕊, 马利雯, 李建武, 杨江伟. 马铃薯Argonaute基因家族鉴定及晚疫病诱导表达分析[J]. 作物学报, 2026, 52(4): 1116-1126. |
| [4] | 张宇, 刘芳, 蔡诚诚, 杨小华, 吉阿么石扎, 杨元军, 王西瑶. 溴乙烷与赤霉素协同处理破除马铃薯块茎休眠的机理初探[J]. 作物学报, 2026, 52(3): 825-838. |
| [5] | 汪玲, 胡好, 宋家凤, 程洁蓝, 陈颖, 郑婷婷, 吕钊彦, 朱晓彪, 侯华兰. 马铃薯UDP-糖基转移酶基因StUGT52的克隆及功能验证[J]. 作物学报, 2026, 52(3): 665-676. |
| [6] | 徐强, 谢奎忠, 胡新元, 岳云, 董博, 罗爱花. 连作对马铃薯根际土壤线虫群落结构与功能的影响[J]. 作物学报, 2026, 52(2): 527-538. |
| [7] | 杨飚, 杜帅康, 张继旺, 石瑛, 张丽莉. 马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析[J]. 作物学报, 2026, 52(2): 405-420. |
| [8] | 詹戈锐, 余文, 李锋, 武明珠, 徐馨, 罗朝鹏, 巫升鑫, 杨军, 张智强, 王中. 烟草NtWRKY6基因响应ABA表达及其调控多酚合成的功能研究[J]. 作物学报, 2026, 52(2): 446-458. |
| [9] | 景秀清, 蔡永朵, 邓宁, 赵晓东, 翟飞红, 曾群. 藜麦RopGEF家族基因的鉴定及表达模式分析[J]. 作物学报, 2026, 52(1): 28-43. |
| [10] | 姬炫彤, 卞春松, 金黎平, 李森, 秦军红, 李广存. 不同耐旱型马铃薯根际微生物对干旱的响应[J]. 作物学报, 2026, 52(1): 165-177. |
| [11] | 田甲春, 葛霞, 李守强, 李梅, 田世龙, 张亚倩, 程建新, 李玉梅. 低O2高CO2贮藏环境延缓马铃薯块茎衰老的作用机制[J]. 作物学报, 2026, 52(1): 262-278. |
| [12] | 王雅致, 杨飚, 季香林, 石瑛, 张丽莉. 二倍体马铃薯抗旱资源鉴定及抗旱基因初步筛选[J]. 作物学报, 2026, 52(1): 72-84. |
| [13] | 卓峰琦, 唐振三, 雷雨俊, 程李香, 赵甜甜, 吕汰, 杨晨, 张峰. 基于烹饪方式及回生温度筛选低升糖马铃薯品种(系)[J]. 作物学报, 2025, 51(9): 2538-2546. |
| [14] | 朱锦程, 杨秋华, 程李香, 李文丽, 石明明, 李惠霞, 张峰. 马铃薯抗南方根结线虫种质资源筛选及相关生理反应分析[J]. 作物学报, 2025, 51(9): 2307-2317. |
| [15] | 尹丽娜, 张锐, 陈国欢, 白磊, 李俊, 郭华春, 杨芳. 不同马铃薯品种块茎创伤愈合能力的比较[J]. 作物学报, 2025, 51(9): 2399-2411. |
|
||