作物学报 ›› 2024, Vol. 50 ›› Issue (8): 2122-2130.doi: 10.3724/SP.J.1006.2024.44006
刘欣玥1,2(), 郭潇阳2, 王欣茹2, 辛大伟1,*(
), 关荣霞2,*(
), 邱丽娟2,*(
)
LIU Xin-Yue1,2(), GUO Xiao-Yang2, WANG Xin-Ru2, XIN Da-Wei1,*(
), GUAN Rong-Xia2,*(
), QIU Li-Juan2,*(
)
摘要:
土壤盐渍化会影响大豆萌发和生长, 筛选萌发期耐盐大豆种质对于耐盐大豆品种选育及盐渍土利用意义重大。本研究以8份代表性大豆种质为材料, 蛭石为培养基质, 用150 mmol L-1 NaCl进行盐胁迫处理, 以水处理作为对照, 测量2 d和3 d的大豆发芽率和芽长, 旨在建立大豆萌发期耐盐性鉴定指标和评价方法。与对照相比, 盐胁迫下大豆发芽率和芽长均下降。盐处理2 d不同种质间相对发芽率、相对芽长无显著差异, 盐处理3 d相对芽长差异显著, 可用以进行不同大豆萌发期耐盐性评价。以150 mmol L-1 NaCl对48份大豆种质进行盐处理, 3 d相对芽长作为评价指标, 将萌发期耐盐性划分为5级, 1级为高度耐盐型, 2级为耐盐型, 3级为中度盐敏感型, 4级为盐敏感型, 5级为高度盐敏感型。鉴定出萌发期高度耐盐种质5份, 耐盐种质12份。同时对48份种质进行出苗期和苗期耐盐性鉴定, 鉴定出3份从萌发期到苗期均耐盐的种质, 分别为中黄685、黑农94和东生104。本研究建立了一套室内大豆萌发期耐盐性鉴定方法, 为耐盐种质鉴定利用奠定了基础。
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