绿豆种质资源苗期耐盐性鉴定及相关基因发掘

doi:10.3724/SP.J.1006.2026.54061

作物学报 ›› 2026, Vol. 52 ›› Issue (2): 376-388.doi: 10.3724/SP.J.1006.2026.54061

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

绿豆种质资源苗期耐盐性鉴定及相关基因发掘

李诗晴^1,2，王茜²，王素华¹，张耀文²，王丽侠^1,*

1 作物基因资源与育种全国重点实验室 / 中国农业科学院作物科学研究所, 北京100081; 2 山西农业大学农学院，山西太谷030801

收稿日期:2025-05-16 修回日期:2025-10-30 接受日期:2025-10-30 出版日期:2026-02-12 网络出版日期:2025-11-12
通讯作者: 王丽侠, E-mail: wanglixia03@caas.cn
基金资助:
本研究由国家重点研发计划项目(2023YFD1200705, 2019YFD1001303), 国家自然科学基金项目(32241042)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-08)资助。

Evaluation of salt tolerance at the seedling stage and related gene mining in mung bean germplasm resources

Li Shi-Qing^1,2,Wang Qian²,Wang Su-Hua¹,Zhang Yao-Wen²,Wang Li-Xia^1,*

1 National Key Laboratory of Crop Gene Resources and Breeding / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China

Received:2025-05-16 Revised:2025-10-30 Accepted:2025-10-30 Published:2026-02-12 Published online:2025-11-12
Contact: 王丽侠, E-mail: wanglixia03@caas.cn
Supported by:
This study was supported by the National Key Research & Development Program of China (2023YFD1200705, 2019YFD1001303), the National Natural Science Foundation of China (32241042), and the China Agriculture Research System of MOF and MARA (CARS-08).

摘要/Abstract

摘要： 土壤盐渍化对农业生产构成严峻威胁，发掘绿豆耐盐种质对充分利用我国盐渍土地和发展绿豆产业均具有重要意义。本研究基于株高、地上部鲜重和根系鲜重等13个性状，对200份绿豆种质进行了苗期耐盐性(150 mmol L^?1 NaCl)鉴定。结果表明，单位体积根长的平均耐盐系数最大(0.806)，根分枝数最小(0.591)；盐胁迫下不同种质对根干重、单位体积根长和根鲜重的响应存在明显差异。根据盐害症状划分的盐害级别，高耐盐种质16份，盐高敏感种质12份，华北区的耐盐种质占比最高；结合重测序数据的全基因组关联分析(GWAS)共检测到67个与绿豆耐盐性显著关联的SNP位点，共涉及348个候选基因，包括99个具有功能注释的基因；经单倍型分析验证，基因Vradi04g09980、Vradi01g06290和Vradi08g02890可能与苗期耐盐性相关。上述结果为进一步开展绿豆耐盐种质创制及解析耐盐调控机制奠定了基础。

关键词: 绿豆, 苗期, 耐盐性, 全基因组关联分析, 种质资源, 单倍型分析

Abstract: Soil salinization poses a serious threat to agricultural production. Exploring salt-tolerant germplasm in mung bean is crucial for the effective utilization of saline-alkali soils in China and for promoting the sustainable development of the mung bean industry. In this study, 200 mung bean germplasm accessions were evaluated for salt tolerance (150 mmol L^?1 NaCl) at the seedling stage based on 13 phenotypic traits, including plant height, above-ground fresh weight, and root fresh weight. The results showed that the salt tolerance coefficient for root length per unit volume was the highest (0.806), while that for root branching was the lowest (0.591). Significant differences were observed among germplasms in their responses to salt stress, particularly in root dry weight, root length per unit volume, and root fresh weight. Based on salt damage symptoms, 16 highly salt-tolerant and 12 highly salt-sensitive germplasms were identified, with the highest proportion of salt-tolerant accessions found in the North China region. A genome-wide association study (GWAS) based on resequencing data identified 67 SNP loci significantly associated with salt tolerance, corresponding to 348 candidate genes, of which 99 had annotated functions. Among these, Vradi04g09980, Vradi01g06290, and Vradi08g02890 were validated through haplotype analysis as potentially associated with salt tolerance at the seedling stage. These findings provide a valuable foundation for the genetic improvement of salt tolerance in mung bean and for further elucidating the underlying regulatory mechanisms.

Key words: mung bean, seedling stage, salt tolerance, genome-wide association study (GWAS), germplasm resources, haplotype

李诗晴, 王茜, 王素华, 张耀文, 王丽侠. 绿豆种质资源苗期耐盐性鉴定及相关基因发掘[J]. 作物学报, 2026, 52(2): 376-388.

Li Shi-Qing, Wang Qian, Wang Su-Hua, Zhang Yao-Wen, Wang Li-Xia. Evaluation of salt tolerance at the seedling stage and related gene mining in mung bean germplasm resources[J]. Acta Agronomica Sinica, 2026, 52(2): 376-388.

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绿豆种质资源苗期耐盐性鉴定及相关基因发掘

Evaluation of salt tolerance at the seedling stage and related gene mining in mung bean germplasm resources

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