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作物学报

作物学报 ›› 2025, Vol. 51 ›› Issue (8): 1991-2008.doi: 10.3724/SP.J.1006.2025.55013

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆不同生育时期耐盐性综合评价及耐盐种质筛选

孟然1(), 李赵嘉1, 冯薇1, 陈悦1, 刘路平2, 杨春燕2, 鲁雪林1, 王秀萍1,*()   

  1. 1河北省农林科学院滨海农业研究所, 河北唐山 063299
    2河北省农林科学院粮油作物研究所 / 国家大豆改良中心石家庄分中心 / 农业农村部黄淮海大豆生物学与遗传育种重点实验室, 河北石家庄 050031
  • 收稿日期:2025-02-13 接受日期:2025-04-25 出版日期:2025-08-12 网络出版日期:2025-05-07
  • 通讯作者: *王秀萍, E-mail: bhswxp@163.com
  • 作者简介:E-mail: yoki_meng@163.com
  • 基金资助:
    唐山市科技计划项目(23150207A);河北省农林科学院现代农业科技创新工程课题项目(2022KJCXZX-BHS-4)

Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm

MENG Ran1(), LI Zhao-Jia1, FENG Wei1, CHEN Yue1, LIU Lu-Ping2, YANG Chun-Yan2, LU Xue-Lin1, WANG Xiu-Ping1,*()   

  1. 1Institute of Coastal Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Tangshan 063299, Hebei, China
    2Institute of Grain and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / Shijiazhuang Branch of National Soybean Improvement Center / Key Laboratory of Soybean Biology and Genetic Breeding, Huanghuaihai, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050031, Hebei, China
  • Received:2025-02-13 Accepted:2025-04-25 Published:2025-08-12 Published online:2025-05-07
  • Contact: *E-mail: bhswxp@163.com
  • Supported by:
    Tangshan Science and Technology Plan Project(23150207A);HAAFS Science and Technology Innovation Special Project(2022KJCXZX-BHS-4)

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摘要：

土壤盐渍化是影响大豆生长的重要非生物胁迫之一。评价与筛选耐盐性大豆种质资源对于挖掘耐盐碱基因、耐盐大豆品种选育及盐碱地高效利用具有重要意义。本研究以50份大豆种质资源为研究对象, 进行了发芽期(0.6% NaCl)、苗期(1.5% NaCl)和全生育期(0.9% NaCl)的耐盐性鉴定, 基于芽期发芽率、苗期株高、叶面积、SPAD值、地上及地下部分的鲜重和干重及丙二醛含量等10个指标和全生育期株高、底荚高度、有效分枝、单株荚数、单株粒数、单株粒重等8个指标, 采用相关性分析、主成分分析、隶属函数法和聚类分析, 对大豆种质耐盐性进行综合评价, 并采用逐步回归分析建立了苗期和全生育期耐盐性预测回归模型, 明确了各时期的耐盐评价指标。结果表明, 依据盐害指数筛选出发芽期高耐盐种质8份, 耐盐种质10份, 高度盐敏感种质6份。利用主成分分析和隶属函数进行综合评价, 鉴定出苗期耐盐材料12份; 通过聚类分析方法将50份全生育期大豆材料分成5类: 高耐盐种质3份、耐盐种质3份、中等耐盐种质20份、盐敏感种质19份和高敏感种质5份。通过逐步回归分析, 建立大豆苗期耐盐性评价预测模型: D= -0.223 + 0.085X1 + 0.203X2 + 0.075X3 + 0.149X6 + 0.132X7 + 0.070X9 + 0.084X10 (R2 = 0.969, P < 0.01)。筛选出相对株高、相对叶面积、相对SPAD、相对地下部分鲜重、相对地下部分干重、相对SOD活性、相对PRO含量可作为大豆苗期耐盐性鉴定的指标。建立大豆全生育期耐盐性评价预测模型: D= -0.153 + 0.143X1 + 0.443X6 + 0.171X7 (R2 = 0.962, P < 0.01), 筛选出相对株高、相对单株粒数、相对单株粒重可以作为大豆全生育期耐盐性评价指标。本研究建立了一套全面精简、针对于大豆不同生育时期的耐盐性鉴定评价方法, 为大豆耐盐机理研究和耐盐新种质选育提供了技术体系和基础材料。

关键词: 大豆, 发芽期, 苗期, 全生育期, 耐盐性, 隶属函数法, 综合评价

Abstract:

Soil salinization is a major abiotic stress that severely hampers soybean growth and productivity. The evaluation and selection of salt-tolerant soybean germplasm are essential for identifying salt-alkali tolerance genes, breeding salt-tolerant cultivars, and improving the efficient use of saline-alkali soils. In this study, 50 soybean germplasm accessions were evaluated for salt tolerance at three developmental stages: germination (0.6% NaCl), seedling (1.5% NaCl), and the full growth period (0.9% NaCl). Salt tolerance was assessed using germination rate at the germination stage; at the seedling stage, ten physiological and morphological indicators were measured, including plant height, leaf area, SPAD value, fresh and dry weights of shoots and roots, and malondialdehyde content. During the full growth stage, eight agronomic traits were evaluated, such as plant height, pod height, number of effective branches, pods per plant, seeds per plant, and seed weight per plant. A comprehensive evaluation was conducted using correlation analysis, principal component analysis (PCA), membership function analysis, and cluster analysis. Stepwise regression was employed to construct predictive models for salt tolerance at the seedling and full-growth stages, identifying key evaluation indicators for each stage. Based on the salt injury index, 8 highly salt-tolerant, 10 salt-tolerant, and 6 highly salt-sensitive germplasms were identified at the germination stage. PCA combined with the membership function approach identified 12 salt-tolerant accessions at the seedling stage. Cluster analysis grouped the 50 accessions into five categories during the full growth stage: 3 highly salt-tolerant, 3 salt-tolerant, 20 moderately salt-tolerant, 19 salt-sensitive, and 5 highly salt-sensitive. The regression model for salt tolerance at the seedling stage was defined as: D = - 0.223 + 0.085X1 + 0.203X2 + 0.075X3 + 0.149X6 + 0.132X7 + 0.070X9 + 0.084X10 (R2 = 0.969, P < 0.01), identifying relative plant height, leaf area, SPAD value, root fresh and dry weights, SOD activity, and proline content as key indicators. For the full growth stage, the model was: D = - 0.153 + 0.143X1 + 0.443X6 + 0.171X7 (R2 = 0.962, P < 0.01), highlighting relative plant height, grain number per plant, and grain weight per plant as critical metrics. This study establishes a comprehensive and systematic framework for evaluating salt tolerance in soybean across different developmental stages and provides a solid technical foundation and valuable germplasm resources for future research on salt tolerance mechanisms and the development of salt-tolerant soybean varieties.

Key words: soybean, germination stage, seedling stage, entire growth stage, salt tolerance, membership function method, comprehensive evaluation

表1

供试大豆种质资源编号及来源"

编号
No.		 品种
Variety		 产地
Origin		 编号
No.		 品种
Variety		 产地
Origin
1 汾豆33 Fendou 33 山西 Shanxi 26 冀2004 Ji 2004 河北 Hebei
2 阜豆36 Fudou 36 安徽 Anhui 27 齐黄51 Qihuang 51 山东 Shandong
3 潍豆10号 Weidou 10 山东 Shandong 28 汾豆99 Fendou 99 山西 Shanxi
4 齐黄30 Qihuang 30 山东 Shandong 29 汾豆100 Fendou 100 山西 Shanxi
5 石豆5号 Shidou 5 河北 Hebei 30 中黄17 Zhonghuang 17 北京 Beijing
6 汾豆56 Fendou 56 山西 Shanxi 31 皖豆43 Wandou 43 安徽 Anhui
7 冀2006 Ji 2006 河北 Hebei 32 邯豆15 Handou 15 河北 Hebei
8 冀豆22 Jidou 22 河北 Hebei 33 冀豆25 Jidou 25 河北 Hebei
9 潍豆126 Weidou 126 山东 Shandong 34 五星1号 Wuxing 1 河北 Hebei
10 晋遗19 Jinyi 19 山西 Shanxi 35 冀豆18 Jidou 18 河北 Hebei
11 中黄56 Zhonghuang 56 北京 Beijing 36 齐黄34 Qihuang 34 山东 Shandong
12 沧豆13 Cangdou 13 河北 Hebei 37 阜豆30 Fudou 30 安徽 Anhui
13 冀豆30 Jidou 30 河北 Hebei 38 晋豆19 Jindou 19 山西 Shanxi
14 冀豆29 Jidou 29 河北 Hebei 39 齐黄21 Qihuang 21 山东 Shandong
15 冀豆20 Jidou 20 河北 Hebei 40 冀2107 Ji 2107 河北 Hebei
16 中黄80 Zhonghuang 80 北京 Beijing 41 皖豆66 Wandou 66 安徽 Anhui
17 冀2115 Ji 2115 河北 Hebei 42 沧豆11 Cangdou 11 河北 Hebei
18 冀豆16 Jidou 16 河北 Hebei 43 晋遗30 Jinyi 30 山西 Shanxi
19 中黄36 Zhonghuang 36 北京 Beijing 44 冀豆17 Jidou17 河北 Hebei
20 冀2206 Ji 2206 河北 Hebei 45 中黄24 Zhonghuang 24 北京 Beijing
21 石豆14 Shidou 14 河北 Hebei 46 五星4号Wuxing 4 河北 Hebei
22 中黄35 Zhonghuang 35 北京 Beijing 47 汾豆20 Fendou 20 山西 Shanxi
23 冀豆23 Jidou 23 河北 Hebei 48 冀青豆2号 Jiqingdou 2 河北 Hebei
24 冀豆26 Jidou 26 河北 Hebei 49 沧豆18 Cangdou 18 河北 Hebei
25 冀豆12 Jidou 12 河北 Hebei 50 中黄74 Zhonghuang 74 北京 Beijing

表2

大豆发芽期耐盐性分级标准"

级别
Level		 相对盐害指数
Relative salt
damage index (%)		 耐盐性
Salt tolerance
1 0.0-20.0 高耐盐 High salt tolerance
2 20.1-40.0 耐盐 Salt tolerance
3 40.1-60.0 中耐盐 Medium salt tolerance
4 60.1-80.0 盐敏感 Salt sensitive
5 80.1-100.0 盐高感 High salt sensitive

图1

大豆发芽期耐盐等级图及发芽表型图 A: 大豆发芽期耐盐等级比例图; B: 耐盐大豆材料发芽期表型图(中黄35); C: 盐敏感大豆材料发芽期表型图(石豆5号)。"

表3

NaCl胁迫下大豆苗期各耐盐指标的描述性统计"

指标
Index		 最大值
Max.		 最小值
Min.		 平均值
Mean		 标准差
SD		 变异系数
CV (%)
相对株高 Relative plant height 0.95 0.43 0.77 0.15 19.55
相对叶面积 Relative leaf area 0.94 0.42 0.66 0.16 24.80
相对SPAD值 Relative SPAD value 1.35 0.29 0.90 0.30 33.37
相对地上部分鲜重 Relative fresh weight of aboveground parts 1.28 0.36 0.85 0.27 31.60
相对地上部分干重 Relative dry weight of aboveground parts 1.33 0.33 0.81 0.28 34.54
相对地下部分鲜重 Relative fresh weight of underground parts 1.40 0.42 0.78 0.21 26.78
相对地下部分干重 Relative dry weight of underground parts 1.48 0.29 0.78 0.30 38.66
相对MDA含量Relative MDA content 1.62 0.51 1.09 0.29 26.11
相对SOD活性Relative SOD activity 1.51 0.55 1.02 0.28 27.15
相对PRO含量Relative PRO content 1.84 0.69 1.19 0.34 28.54

表4

NaCl胁迫下大豆苗期各耐盐指标的相关性分析"

指标
Item		 相对株高Relative plant height 相对叶面积Relative leaf area 相对SPAD值 Relative SPAD value 相对地上部分鲜重Relative fresh weight of aboveground parts 相对地上部分干重Relative dry weight of aboveground parts 相对地下部分鲜重Relative fresh weight of underground parts 相对地下部分干重Relative dry weight of underground parts 相对MDA Relative MDA content 相对SOD Relative SOD content 相对PRO Relative PRO content
相对株高
Relative plant height		 1.000
相对叶面积
Relative leaf area		 0.191 1.000
相对SPAD值
Relative SPAD value		 0.642** 0.165 1.000
相对地上部分鲜重Relative fresh weight of aboveground parts 0.061 0.053 0.042 1.000
相对地上部分干重Relative dry weight of aboveground parts 0.011 0.086 −0.049 0.727** 1.000
相对地下部分鲜重Relative fresh weight of underground parts 0.155 0.125 0.046 0.010 0.025 1.000
相对地下部分干重Relative dry weight of underground parts 0.001 0.240 −0.025 0.168 0.341* 0.257 1.000
相对MDA
Relative MDA content		 −0.132 −0.454** −0.106 −0.382** −0.351* −0.132 −0.192 1.000
相对SOD
Relative SOD content		 −0.167 −0.565** −0.208 −0.243 −0.286 −0.207 −0.193 0.642** 1.000
相对PRO
Relative PRO content		 −0.198 −0.615** −0.198 −0.236 −0.208 −0.182 −0.213 0.703** 0.900** 1.000

表5

大豆苗期各耐盐指标主成分系数、贡献率及主成分载荷矩阵"

指标
Item		 主成分因子 Principal component factor
F1 F2 F3 F4
相对株高 Relative plant height 0.511 0.177 0.429 -0.577
相对叶面积 Relative leaf area 0.840 -0.159 -0.133 0.316
相对SPAD值 Relative SPAD value 0.490 0.151 0.480 -0.572
相对地上部分鲜重 Relative fresh weight of aboveground parts 0.180 0.585 -0.646 -0.288
相对地上部分干重 Relative dry weight of aboveground parts 0.146 0.695 -0.597 -0.090
相对地下部分鲜重 Relative fresh weight of underground parts 0.336 0.599 0.483 0.379
相对地下部分干重 Relative underground dry weight 0.318 0.655 0.307 0.407
相对MDA含量 Relative MDA content -0.704 0.266 0.272 0.182
相对SOD活性 Relative SOD activity 0.888 -0.239 -0.101 0.096
相对PRO含量 Relative PRO content 0.899 -0.275 -0.131 0.179
特征值 Eigenvalue 3.567 1.896 1.635 1.235
贡献率 Contribution ratio (%) 35.674 18.960 16.347 12.350
累积贡献率 Cumulative contribution ratio (%) 35.674 54.634 70.981 83.331

表6

大豆苗期耐盐性综合评价"

编号
Code		 综合指标值
Comprehensive index		 隶属函数值
Subordinate function value		 D值
D-value		 耐盐排序
Salt
tolerance ranking		 耐盐等级
Salt resistance grade
CI(1) CI(2) CI(3) CI(4) U(1) U(2) U(3) U(4)
1 1.79 1.98 0.32 -0.52 0.70 0.87 0.60 0.33 0.66 8 II级耐盐 Grade II salt tolerance
2 1.27 -1.21 -1.06 -0.78 0.63 0.32 0.35 0.27 0.45 26 III级中间型 Grade III intermediate type
3 -1.64 -1.24 -0.97 -0.66 0.20 0.31 0.37 0.30 0.19 47 V级盐敏感 Grade V salt sensitive
4 1.41 -2.12 0.37 1.03 0.65 0.16 0.61 0.63 0.53 17 III级中间型 Grade III intermediate type
5 -0.01 -3.05 -1.11 0.48 0.44 0.00 0.34 0.52 0.33 42 IV级不耐盐 Grade IV salt intolerance
6 -0.09 -1.08 -0.37 -0.46 0.43 0.34 0.48 0.34 0.40 34 III级中间型 Grade III intermediate type
7 -1.88 -1.29 0.32 -0.54 0.17 0.30 0.60 0.32 0.31 44 IV级不耐盐 Grade IV salt intolerance
8 1.69 0.22 0.68 2.05 0.69 0.56 0.67 0.83 0.68 6 II级耐盐 Grade II salt tolerance
9 2.35 -0.81 -3.02 -0.17 0.79 0.39 0.00 0.39 0.48 21 III级中间型 Grade III intermediate type
10 0.64 -1.20 2.53 -0.05 0.54 0.32 1.00 0.42 0.56 14 III级中间型 Grade III intermediate type
11 1.38 -0.81 0.04 -2.06 0.64 0.39 0.55 0.02 0.47 22 III级中间型 Grade III intermediate type
12 -0.04 2.52 0.61 -0.67 0.44 0.96 0.65 0.30 0.58 13 III级中间型 Grade III intermediate type
13 -2.13 -1.54 2.13 -1.14 0.13 0.26 0.93 0.20 0.33 43 IV级不耐盐 Grade IV salt intolerance
14 -2.29 -0.12 1.14 2.24 0.11 0.51 0.75 0.87 0.44 30 III级中间型 Grade III intermediate type
15 -1.91 -1.02 0.97 2.03 0.16 0.35 0.72 0.83 0.41 33 III级中间型 Grade III intermediate type
16 0.31 -1.53 -0.97 -0.04 0.49 0.26 0.37 0.42 0.40 35 III级中间型 Grade III intermediate type
17 -3.03 0.02 -1.11 -0.89 0.00 0.53 0.34 0.25 0.16 50 V级盐敏感 Grade V salt sensitive
18 -0.35 0.70 0.83 0.00 0.39 0.65 0.69 0.43 0.51 19 III级中间型 Grade III intermediate type
19 -1.43 -0.44 1.44 -0.75 0.23 0.45 0.80 0.28 0.40 36 III级中间型 Grade III intermediate type
20 -1.30 1.33 -0.91 0.75 0.25 0.76 0.38 0.58 0.44 29 III级中间型 Grade III intermediate type
21 1.28 -1.50 -1.55 -0.16 0.63 0.27 0.26 0.40 0.44 28 III级中间型 Grade III intermediate type
22 1.49 1.49 0.07 -0.89 0.66 0.78 0.56 0.25 0.61 11 II级耐盐 Grade II salt tolerance
23 3.25 0.14 -1.24 -1.02 0.92 0.55 0.32 0.23 0.61 9 II级耐盐 Grade II salt tolerance
24 3.63 -0.16 0.80 0.52 0.97 0.50 0.69 0.53 0.74 4 II级耐盐 Grade II salt tolerance
25 2.58 0.28 2.29 1.24 0.82 0.57 0.96 0.67 0.77 1 II级耐盐 Grade II salt tolerance
26 2.02 -1.90 -0.77 0.89 0.74 0.20 0.41 0.60 0.53 16 III级中间型 Grade III intermediate type
27 -1.85 -1.41 1.13 -1.49 0.17 0.28 0.75 0.13 0.30 45 IV级不耐盐 Grade IV salt intolerance
28 -2.24 -1.21 -0.16 -1.36 0.12 0.32 0.52 0.16 0.17 49 V级盐敏感 Grade V salt sensitive
29 -1.59 0.26 1.22 1.13 0.21 0.57 0.76 0.65 0.47 24 III级中间型 Grade III intermediate type
30 -1.98 0.60 0.21 2.90 0.15 0.63 0.58 1.00 0.47 23 III级中间型 Grade III intermediate type
31 -1.33 0.40 -1.07 0.47 0.25 0.59 0.35 0.52 0.39 38 IV级不耐盐 Grade IV salt intolerance
32 -2.12 0.94 -2.44 -0.97 0.13 0.69 0.10 0.24 0.18 48 V级盐敏感 Grade V salt sensitive
33 -0.29 0.15 -1.91 0.99 0.40 0.55 0.20 0.62 0.43 32 III级中间型 Grade III intermediate type
34 -1.64 2.06 -0.88 0.23 0.20 0.88 0.39 0.47 0.43 31 III级中间型 Grade III intermediate type
35 -2.37 2.49 -1.23 0.33 0.10 0.96 0.32 0.49 0.39 37 IV级不耐盐 Grade IV salt intolerance
36 3.80 0.21 0.75 0.32 1.00 0.56 0.68 0.49 0.76 2 II级耐盐 Grade II salt tolerance
37 -1.06 2.75 -0.75 1.09 0.29 1.00 0.41 0.64 0.53 18 III级中间型 Grade III intermediate type
38 -2.04 -1.21 1.37 -2.17 0.14 0.32 0.79 0.00 0.29 46 IV级不耐盐 Grade IV salt intolerance
39 3.82 1.02 -0.10 -0.34 1.00 0.70 0.53 0.36 0.74 3 II级耐盐 Grade II salt tolerance
40 -0.43 1.39 -1.51 -0.40 0.38 0.77 0.27 0.35 0.44 27 III级中间型 Grade III intermediate type
41 -2.15 -0.08 0.55 0.75 0.13 0.51 0.64 0.58 0.38 39 IV级不耐盐 Grade IV salt intolerance
42 0.11 -1.66 -2.62 1.87 0.46 0.24 0.07 0.80 0.38 40 IV级不耐盐 Grade IV salt intolerance
43 0.90 -0.60 -0.03 -0.93 0.57 0.42 0.54 0.24 0.48 20 III级中间型 Grade III intermediate type
44 1.91 1.25 0.56 0.34 0.72 0.74 0.65 0.50 0.68 7 II级耐盐 Grade II salt tolerance
45 -1.42 0.29 -0.44 -1.03 0.24 0.58 0.46 0.22 0.36 41 IV级不耐盐 Grade IV salt intolerance
46 -1.10 -0.85 1.90 0.35 0.28 0.38 0.89 0.50 0.45 25 III级中间型 Grade III intermediate type
47 1.44 1.40 0.21 -0.87 0.65 0.77 0.58 0.26 0.61 12 II级耐盐 Grade II salt tolerance
48 -0.51 2.75 0.80 -1.42 0.37 1.00 0.69 0.15 0.54 15 III级中间型 Grade III intermediate type
49 2.71 0.41 0.99 -0.10 0.84 0.60 0.72 0.41 0.70 5 II级耐盐 Grade II salt tolerance
50 0.45 0.99 2.00 -0.11 0.51 0.70 0.90 0.41 0.61 10 II级耐盐 Grade II salt tolerance

表7

NaCl胁迫下大豆全生育期各耐盐指标的描述性统计"

指标
Item		 最大值
Max.		 最小值
Min.		 平均值
Mean		 标准差
SD		 变异系数
CV (%)
相对株高 Relative plant height 1.24 0.46 0.85 0.21 25.22
相对底荚高度 Relative bottom pod height 1.15 0.33 0.73 0.22 30.86
相对主茎节数 Relative number of main stem nodes 1.22 0.44 0.76 0.17 22.89
相对有效分枝 Relatively effective branching 1.28 0.46 0.80 0.21 26.52
相对单株荚数 Relative number of pods per plant 1.55 0.26 0.80 0.30 37.59
相对单株粒数 Relative number of grains per plant 1.64 0.24 0.82 0.33 40.55
相对单株粒重 Relative grain weight per plant 1.54 0.16 0.63 0.31 49.08
相对百粒重 Relative weight per hundred grains 1.34 0.13 0.66 0.29 43.42

表8

NaCl胁迫下大豆全生育期各耐盐指标的相关性分析"

性状
Trait		 相对株高Relative plant height 相对底荚
高度Relative bottom pod height		 相对主茎
节数
Relative number of main stem nodes		 相对有效
分枝Relatively effective branching		 相对单株
荚数Relative number of pods per plant		 相对单株
粒数Relative number of grains per plant		 相对单株
粒重Relative grain weight per plant		 相对百粒重Relative weight per hundred grains
相对株高
Relative plant height		 1.000
相对底荚高度
Relative bottom pod height		 -0.006 1.000
相对主茎节数
Relative number of main stem nodes		 0.184 0.149 1.000
相对有效分枝
Relatively effective branching		 -0.001 0.773** 0.093 1.000
相对单株荚数
Relative number of pods per plant		 -0.018 0.902** 0.241 0.876** 1.000
相对单株粒数
Relative number of grains per plant		 0.078 0.920** 0.244 0.841** 0.958** 1.000
相对单株粒重
Relative grain weight per plant		 0.134 -0.124 -0.016 -0.099** -0.116 -0.143 1.000
相对百粒重
Relative weight per hundred grains		 0.015 0.097 -0.079 0.079 0.050 0.072 0.707** 1.000

表9

大豆全生育期各耐盐指标主成分系数、贡献率及主成分载荷矩阵"

指标
Item		 主成分因子Principal component factor
F1 F2 F3
相对株高 Relative plant height 0.029 0.136 0.788
相对底荚高度 Relative bottom pod height 0.939 0.050 -0.080
相对主茎节数 Relative number of main stem nodes 0.257 -0.191 0.717
相对有效分枝 Relatively effective branching 0.904 0.060 -0.112
相对单株荚数 Relative number of pods per plant 0.980 0.021 -0.032
相对单株粒数 Relative number of grains per plant 0.979 0.024 0.032
相对单株粒重 Relative grain weight per plant -0.158 0.898 0.165
相对百粒重 Relative weight per hundred grains 0.045 0.931 -0.115
特征值 Eigenvalue 3.711 1.736 1.198
贡献率 Contribution ratio (%) 46.392 21.699 14.971
累积贡献率 Cumulative contribution ratio (%) 46.392 68.091 83.061

表10

大豆全生育期耐盐性综合评价"

编号
Code		 综合指标值
Comprehensive index		 隶属函数值
Subordinate function value		 D值
D-value		 耐盐排序Salt
tolerance ranking		 耐盐等级
Salt resistance grade
CI(1) CI(2) CI(3) U(1) U(2) U(3)
1 -0.82 -0.54 -1.13 0.30 0.33 0.29 0.31 41 IV级不耐盐 Grade IV salt intolerance
2 -2.87 -1.13 -0.66 0.04 0.23 0.40 0.15 50 V级盐敏感 Grade V salt sensitive
3 -2.07 1.18 -0.02 0.14 0.61 0.56 0.34 39 IV级不耐盐 Grade IV salt intolerance
4 -0.29 1.33 -1.98 0.37 0.63 0.08 0.39 30 IV级不耐盐 Grade IV salt intolerance
5 -1.23 -1.35 1.21 0.25 0.19 0.86 0.34 38 IV级不耐盐 Grade IV salt intolerance
6 -1.85 -0.59 0.19 0.17 0.32 0.61 0.29 44 IV级不耐盐 Grade IV salt intolerance
7 1.14 0.02 -2.24 0.56 0.42 0.02 0.43 26 III级中间型 Grade III intermediate type
8 -1.32 -0.07 -0.79 0.24 0.40 0.37 0.31 42 IV级不耐盐 Grade IV salt intolerance
9 -1.03 0.32 -0.18 0.28 0.47 0.52 0.37 33 IV级不耐盐 Grade IV salt intolerance
10 -0.71 -0.07 -0.22 0.32 0.40 0.51 0.37 32 IV级不耐盐 Grade IV salt intolerance
11 1.10 0.52 -1.54 0.55 0.50 0.19 0.47 18 III级中间型 Grade III intermediate type
12 0.31 -2.11 0.53 0.45 0.07 0.69 0.39 28 IV级不耐盐 Grade IV salt intolerance
13 -0.96 -1.32 1.15 0.28 0.20 0.84 0.36 34 IV级不耐盐 Grade IV salt intolerance
14 2.71 1.04 0.97 0.76 0.58 0.80 0.72 4 II级耐盐 Grade II salt tolerance
15 0.42 1.25 -0.56 0.47 0.62 0.43 0.50 16 III级中间型 Grade III intermediate type
16 -3.14 0.21 -1.21 0.00 0.45 0.27 0.17 49 V级盐敏感 Grade V salt sensitive
17 -1.06 0.09 -1.48 0.27 0.43 0.21 0.30 43 IV级不耐盐 Grade IV salt intolerance
18 -1.27 1.78 0.90 0.24 0.71 0.78 0.46 21 III级中间型 Grade III intermediate type
19 1.63 0.70 -0.48 0.62 0.53 0.45 0.57 8 III级中间型Grade III intermediate type
20 2.06 3.58 0.75 0.68 1.00 0.74 0.77 3 I级高耐盐 Grade I high salt tolerance
21 0.51 0.20 0.24 0.48 0.45 0.62 0.50 17 III级中间型 Grade III intermediate type
22 3.78 0.30 1.55 0.90 0.46 0.94 0.80 2 I级高耐盐 Grade I high salt tolerance
23 -2.62 1.52 0.36 0.07 0.66 0.65 0.33 40 IV级不耐盐 Grade IV salt intolerance
24 -0.88 2.10 -0.63 0.30 0.76 0.41 0.44 25 III级中间型 Grade III intermediate type
25 1.09 -0.88 1.32 0.55 0.27 0.88 0.54 11 III级中间型 Grade III intermediate type
26 -2.05 -1.68 -0.48 0.14 0.14 0.45 0.20 47 V级盐敏感 Grade V salt sensitive
27 -0.26 -0.31 -0.74 0.38 0.36 0.38 0.37 31 IV级不耐盐 Grade IV salt intolerance
28 -0.43 1.06 0.19 0.35 0.59 0.61 0.46 22 III级中间型 Grade III intermediate type
29 0.26 -0.03 1.49 0.44 0.41 0.92 0.52 14 III级中间型 Grade III intermediate type
30 -0.81 1.93 1.79 0.30 0.73 1.00 0.54 10 III级中间型 Grade III intermediate type
31 -2.56 2.38 0.05 0.08 0.80 0.57 0.36 36 IV级不耐盐 Grade IV salt intolerance
32 -0.99 0.25 1.62 0.28 0.45 0.95 0.45 23 III级中间型 Grade III intermediate type
33 4.48 1.47 1.42 1.00 0.65 0.91 0.89 1 I级高耐盐 Grade I high salt tolerance
34 1.42 0.07 0.46 0.60 0.43 0.67 0.57 9 III级中间型Grade III intermediate type
35 2.18 -1.56 -0.21 0.70 0.16 0.51 0.52 13 III级中间型Grade III intermediate type
36 -1.47 -1.09 -0.29 0.22 0.24 0.49 0.27 45 IV级不耐盐Grade IV salt intolerance
37 -0.16 -1.33 -0.42 0.39 0.20 0.46 0.35 37 IV级不耐盐Grade IV salt intolerance
38 3.09 -0.71 -2.33 0.81 0.30 0.00 0.53 12 III级中间型Grade III intermediate type
39 -0.68 2.72 -1.47 0.32 0.86 0.21 0.44 24 III级中间型Grade III intermediate type
40 0.31 -0.79 -0.58 0.45 0.28 0.42 0.40 27 IV级不耐盐Grade IV salt intolerance
41 -3.08 -0.64 0.14 0.01 0.31 0.60 0.19 48 V级盐敏感Grade V salt sensitive
42 1.02 -1.64 0.42 0.54 0.15 0.66 0.46 20 III级中间型Grade III intermediate type
43 0.33 -0.91 1.81 0.45 0.27 1.00 0.50 15 III级中间型Grade III intermediate type
44 4.51 -0.49 -1.61 1.00 0.33 0.17 0.68 5 II级耐盐Grade II salt tolerance
45 2.23 -0.52 -0.02 0.70 0.33 0.56 0.58 7 III级中间型Grade III intermediate type
46 -2.92 -1.74 1.12 0.03 0.13 0.83 0.20 46 V级盐敏感Grade V salt sensitive
47 1.00 -2.53 -1.06 0.54 0.00 0.31 0.36 35 IV级不耐盐Grade IV salt intolerance
48 2.99 -0.82 0.47 0.80 0.28 0.68 0.64 6 II级耐盐Grade II salt tolerance
49 -1.04 -0.66 1.24 0.27 0.31 0.86 0.39 29 IV级不耐盐Grade IV salt intolerance
50 0.02 -0.52 1.13 0.41 0.33 0.84 0.47 19 III级中间型Grade III intermediate type

图2

大豆全生育期耐盐性聚类分析"

表11

各时期耐盐及以上等级的大豆品种"

耐盐等级
Salt tolerance level		 发芽期耐盐大豆
Salt tolerant soybeans during germination stage		 苗期耐盐大豆
Salt tolerant soybean during
seedling stage		 全生育期耐盐大豆
Salt tolerant soybeans throughout their entire growth stage
高耐盐
High salt tolerance		 1, 9, 22, 23, 36, 44, 48, 49
 —
 33, 22, 20
耐盐
Salt tolerance		 2, 5, 12, 15, 17, 24, 27, 30, 33, 40
 25, 36, 39, 24, 49, 8, 44, 1, 23, 50, 22, 47 14, 44, 48

图3

大豆发芽期、苗期和全生育期耐盐品种差异分析"

表12

各时期耐盐等级相关性分析"

时期
Stage		 发芽期
Germination stage		 苗期
Seedling stage		 全生育期
Entire growth stage
发芽期 Germination stage 1.000
苗期 Seedling stage 0.175 1.000
全生育期
Entire growth stage		 -0.081
 0.111
 1.000

图4

不同NaCl浓度胁迫大豆品种耐盐性四分位图 图A为发芽期大豆品种盐害指数四分位图; 图B为苗期大豆品种D值四分位图; 图C为全生育期大豆品种D值四分位图。"

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