欢迎访问作物学报，今天是
作物学报

作物学报

• •    

大豆不同生育时期耐盐性综合评价及耐盐种质筛选

孟然1，李赵嘉1，冯薇1，陈悦1，刘路平2，杨春燕2，鲁雪林1，王秀萍1,*   

  1. 1 河北省农林科学院滨海农业研究所，河北唐山 063299；2 河北省农林科学院粮油作物研究所 / 国家大豆改良中心石家庄分中心 / 农业农村部黄淮海大豆生物学与遗传育种重点实验室，河北石家庄 050031
  • 收稿日期:2025-02-13 修回日期:2025-04-25 接受日期:2025-04-25 网络出版日期:2025-05-07
  • 基金资助:
    本研究由唐山市科技计划项目(23150207A)和河北省农林科学院现代农业科技创新工程课题项目(2022KJCXZX-BHS-4)资助。

Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm

MENG Ran1,LI Zhao-Jia1,FENG Wei1,CHEN Yue1,LIU Lu-Ping2,YANG Chun-Yan2,LU Xue-Lin1,WANG Xiu-Ping1,*   

  1. 1 Institute of Coastal Agriculture, Hebei Academy of Agricultural and Forestry Sciences, Tangshan 063299, Hebei, China; 2 Institute of Grain and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / Shijiazhuang Branch of National Soybean Improvement Center / Key Laboratory of Soybean Biology and Genetic Breeding, Huanghuaihai, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050031, Hebei, China
  • Received:2025-02-13 Revised:2025-04-25 Accepted:2025-04-25 Published online:2025-05-07
  • Supported by:
    This study was supported by the Tangshan Science and Technology Plan Project (23150207A) and the HAAFS Science and Technology Innovation Special Project (2022KJCXZX-BHS-4).

PDF

27

摘要：

土壤盐渍化是影响大豆生长的重要非生物胁迫之一。评价与筛选耐盐性大豆种质资源对于挖掘耐盐碱基因、耐盐大豆品种选育及盐碱地高效利用具有重要意义。本研究以50份大豆种质资源为研究对象，进行了发芽期(0.6% NaCl)、苗期(1.5% NaCl)和全生育期(0.9% NaCl)的耐盐性鉴定，通过测定芽期发芽率、苗期株高、叶面积、SPAD值、地上及地下部分的鲜重和干重及丙二醛含量等10个指标和全生育期株高、底荚高度、有效分枝、单株荚数、单株粒数、单株粒重等8个指标，采用相关性分析、主成分分析、隶属函数法和聚类分析，对大豆种质耐盐性进行综合评价，并采用逐步回归分析建立了苗期和全生育期耐盐性预测回归模型，明确了各时期的耐盐评价指标。结果表明，依据盐害指数筛选出发芽期高耐盐种质8份，耐盐种质10份，高度盐敏感种质6份。利用主成分分析和隶属函数进行综合评价，鉴定出苗期耐盐材料12份；通过聚类分析方法将50份全生育期大豆材料分成5类：高耐盐种质3份、耐盐种质3份、中等耐盐种质20份、盐敏感种质19份和高敏感种质5份。通过逐步回归分析，建立大豆苗期耐盐性评价预测模型：D= – 0.223 + 0.085X1 + 0.203X2 + 0.075X3 + 0.149X6 + 0.132X7 + 0.070X9 + 0.084X10 (R2=0.969，P<0.01)，筛选出相对株高、相对叶面积、相对SPAD、相对地下部分鲜重、相对地下部分干重、相对SOD、相对PRO可作为大豆苗期耐盐性鉴定的指标。建立大豆全生育期耐盐性评价预测模型：D= – 0.153 + 0.143X1 + 0.443X6 + 0.171X7 (R2=0.962，P<0.01)，筛选出相对株高、相对单株粒数、相对单株粒重可以作为大豆全生育期耐盐性评价指标。本研究建立了一套全面精简、针对于大豆不同生育时期的耐盐性鉴定评价方法，为大豆耐盐机理研究和耐盐新种质选育提供了技术体系和基础材料。

关键词: 大豆, 发芽期, 苗期, 全生育期, 耐盐性, 隶属函数法, 综合评价

Abstract:

土壤盐渍化是影响大豆生长的重要非生物胁迫之一。评价与筛选耐盐性大豆种质资源对于挖掘耐盐碱基因、耐盐大豆品种选育及盐碱地高效利用具有重要意义。本研究以50份大豆种质资源为研究对象，进行了发芽期(0.6% NaCl)、苗期(1.5% NaCl)和全生育期(0.9% NaCl)的耐盐性鉴定，通过测定芽期发芽率、苗期株高、叶面积、SPAD值、地上及地下部分的鲜重和干重及丙二醛含量等10个指标和全生育期株高、底荚高度、有效分枝、单株荚数、单株粒数、单株粒重等8个指标，采用相关性分析、主成分分析、隶属函数法和聚类分析，对大豆种质耐盐性进行综合评价，并采用逐步回归分析建立了苗期和全生育期耐盐性预测回归模型，明确了各时期的耐盐评价指标。结果表明，依据盐害指数筛选出发芽期高耐盐种质8份，耐盐种质10份，高度盐敏感种质6份。利用主成分分析和隶属函数进行综合评价，鉴定出苗期耐盐材料12份；通过聚类分析方法将50份全生育期大豆材料分成5类：高耐盐种质3份、耐盐种质3份、中等耐盐种质20份、盐敏感种质19份和高敏感种质5份。通过逐步回归分析，建立大豆苗期耐盐性评价预测模型：D= – 0.223 + 0.085X1 + 0.203X2 + 0.075X3 + 0.149X6 + 0.132X7 + 0.070X9 + 0.084X10 (R2=0.969，P<0.01)，筛选出相对株高、相对叶面积、相对SPAD、相对地下部分鲜重、相对地下部分干重、相对SOD、相对PRO可作为大豆苗期耐盐性鉴定的指标。建立大豆全生育期耐盐性评价预测模型：D= – 0.153 + 0.143X1 + 0.443X6 + 0.171X7 (R2=0.962，P<0.01)，筛选出相对株高、相对单株粒数、相对单株粒重可以作为大豆全生育期耐盐性评价指标。本研究建立了一套全面精简、针对于大豆不同生育时期的耐盐性鉴定评价方法，为大豆耐盐机理研究和耐盐新种质选育提供了技术体系和基础材料。

Key words: soybean, germination stage, seedling stage, entire growth stage, salt tolerance, membership function method, comprehensive evaluation

[1] Maria G, Petropoulos Spyridon A, Youssef R.. Response and defence mechanisms of vegetable crops against drought, heat and salinity stress. Agriculture, 2021, 11: 463.

[2] Hopmans J W, Qureshi A S, Kisekka I, Munns R, Grattan S R, Rengasamy P, Ben-Gal A, Assouline S, Javaux M, Minhas P S, et al. Critical knowledge gaps and research priorities in global soil salinity. Adv Agron, 2021, 169: 1–191.

[3] 王炳春. 现阶段我国开展盐碱地综合利用对策研究. 中国农垦, 2024, (1): 52–54.
Wang B C. Study on countermeasures of comprehensive utilization of saline-alkali land in China at the present stage. China State Farm, 2024, (1): 52–54 (in Chinese).

[4] Yang Y Q, Guo Y. Elucidating the molecular mechanisms mediating plant salt-stress responses. New Phytol, 2018, 217: 523–539.

[5] Li W X, Pang S Y, Lu Z G, Jin B. Function and mechanism of WRKY transcription factors in abiotic stress responses of plants. Plants, 2020, 9: 1515.

[6] Zhou X Y, Tian Y M, Qu Z P, Wang J X, Han D Z, Dong S K. Comparing the salt tolerance of different spring soybean varieties at the germination stage. Plants, 2023, 12: 2789.

[7] Chen L M, Peng L H, Ouyang W Q, Yao H W, Ye Y X, Shan Z H, Cao D, Chen S L, Yang Z L, Huang Y, et al. Screening and identification of salt tolerance soybean varieties and germplasms. Oil Crop Sci, 2024, 9: 204–210.

[8] 刘谢香, 常汝镇, 关荣霞, 邱丽娟. 大豆出苗期耐盐性鉴定方法建立及耐盐种质筛选. 作物学报, 2020, 46: 1–8.
Liu X X, Chang R Z, Guan R X, Qiu L J. Establishment of screening method for salt tolerant soybean at emergence stage and screening of tolerant germplasm. Acta Agron Sin, 2020, 46: 1–8 (in Chinese with English abstract).

[9] 张兆宁, 李江辉, 赵怡宇, 范亚茹, 杜艳丽, 韩德志, 张玉先, 杜吉到. 不同程度盐胁迫下大豆萌发期耐盐性鉴定. 大豆科学, 2023, 42: 335–343.
Zhang Z N, Li J H, Zhao Y Y, Fan Y R, Du Y L, Han D Z, Zhang Y X, Du J D. Salt tolerance identification of soybean at germination stage under different salt stress degrees. Soybean Sci, 2023, 42: 335–343 (in Chinese with English abstract).

[10] 周秀文, 张晓蕊, 孙贺祥, 赵翔, 张娜, 姚兴东, 谢甫绨. 大豆种质萌发期和苗期耐盐性评价. 沈阳农业大学学报, 2022, 53(3): 257–264.
Zhou X W, Zhang X R, Sun H X, Zhao X, Zhang N, Yao X D, Xie F T. Evaluation of salt tolerance of soybean germplasms at germination and seedling stages. J Shenyang Agric Univ, 2022, 53(3): 257–264 (in Chinese with English abstract).

[11] 林峰, 赵慧艳, 史飞飞, 高鹏, 刘晨煦, 岳阳, 金昕, 张意德, 李永光, 韩英鹏. 大豆种质资源苗期耐盐鉴定及遗传多样性分析. 植物遗传资源学报, 2024, 25: 945−956.
Lin F, Zhao H Y, Shi F F, Gao P, Liu C X, Yue Y, Jin X, Zhang Y D, Li Y G, Han Y P. Identification of salt-tolerant germplasm resources in soybean seedlings and genetic diversity analysis. J Plant Genet Resour, 2024, 25: 945−956 (in Chinese with English abstract).

[12] 韩岱, 时晓磊, 丁孙磊, 张金波, 严勇亮. 60份大豆种质资源苗期耐盐性鉴定评价. 大豆科学, 2023, 42: 494–505.
Han D, Shi X L, Ding S L, Zhang J B, Yan Y L. Evaluation of salt tolerance in 60 soybean germplasm resources at seedling stage. Soybean Sci, 2023, 42: 494–505 (in Chinese with English abstract).

[13] 姜奇彦, 胡正, 张辉, 王萌萌, 唐俊源, 倪志勇, 姜锋. 大豆种质资源耐盐性鉴定与研究. 植物遗传资源学报, 2012, 13: 726–732.
Jiang Q Y, Hu Z, Zhang H, Wang M M, Tang J Y, Ni Z Y, Jiang F. Evaluation for salt tolerance in soybean cultivars (Glycine max L. Merrill). J Plant Genet Resour, 2012, 13: 726–732 (in Chinese with English abstract).

[14] 徐宗昌, 鲁雪莉, 魏云冲, 孟晨, 张梦超, 张缘杨, 王萌, 王菊英, 张成省, 李义强. 航天诱变野大豆SP1群体苗期耐盐性鉴定与评价. 草业学报, 2023, 32(11): 168–178.
Xu Z C, Lu X L, Wei Y C, Meng C, Zhang M C, Zhang Y Y, Wang M, Wang J Y, Zhang C S, Li Y Q. Salt tolerance identification and evaluation of a population of wild soybean SP1 mutants at the seedling stage. Acta Pratac Sin, 2023, 32(11): 168–178 (in Chinese with English abstract).

[15] 袁宇婷, 张晓燕, 吴谷丰, 黄璐, 袁星星, 陈新, 刘晓勇, 薛晨晨. 基于主成分和隶属函数分析的大豆种质资源耐盐性综合评价. 大豆科学, 2025, 44: 22–32.
Yuan Y T, Zhang X Y, Wu G F, Huang L, Yuan X X, Chen X, Liu X Y, Xue C C. Comprehensive evaluation of salt tolerance of soybean germplasm resources based on principal component and membership function analysis. Soybean Sci, 2025, 44: 22–32 (in Chinese with English abstract).

[16] 孙现军, 胡正, 姜雪敏, 王世佳, 陈向前, 张惠媛, 张辉, 姜奇彦. 大豆种质资源苗期耐盐性鉴定评价与筛选. 作物学报, 2024, 50: 2179–2186.
Sun X J, Hu Z, Jiang X M, Wang S J, Chen X Q, Zhang H Y, Zhang H, Jiang Q Y. Identification, evaluation and screening of salt-tolerant of soybean germplasm resources at seedling stage. Acta Agron Sin, 2024, 50: 2179–2186 (in Chinese with English abstract).

[17] Jameel J, Anwar T, Majeed S, Qureshi H, Siddiqi E H, Sana S, Zaman W, Ali H M. Effect of salinity on growth and biochemical responses of brinjal varieties: implications for salt tolerance and antioxidant mechanisms. BMC Plant Biol, 2024, 24: 128.

[18] Wei H, Movahedi A, Liu G Y, Li Y X, Liu S W, Yu C M, Chen Y H, Zhong F, Zhang J. Comprehensive analysis of carotenoid cleavage dioxygenases gene family and its expression in response to abiotic stress in poplar. Int J Mol Sci, 2022, 23: 1418.

[19] 田戈, 南丽丽, 杨丽丽, 郭佳雨, 马香香. 红豆草苗期耐盐种质鉴定及综合评价. 草地学报, 网络首发[2025-03-17], http://kns.cnki.net/kcms/detail/11.3362.S.20241230.1441.004.html.
Tian G, Nan L L, Yang L L, Guo J Y, Ma X X. Screening and evaluation of salt-tolerant germplasm of sainfoin at seedling stage. J Grassl Sci, Published online [2025-03-07], http://kns.cnki.net/kcms/detail/11.3362.S.20241230.1441.004.html (in Chinese with English abstract).

[20] Zulfiqar F, Ashraf M. Proline alleviates abiotic stress induced oxidative stress in plants. J Plant Growth Regul, 2023, 42: 4629–4651.

[21] 张海平, 张俊峰, 陈妍, 张海生, 闫凯, 穆志新. 大豆种质资源萌发期耐旱性评价. 植物遗传资源学报, 2021, 22: 130–138.
Zhang H P, Zhang J F, Chen Y, Zhang H S, Yan K, Mu Z X. Identification and evaluation of soybean germplasm resources for drought tolerance during germination stage. J Plant Genet Resour, 2021, 22: 130–138 (in Chinese with English abstract).

[22] Suo R Z, Kulbir S, You F, Conner R, Cober E, Wang M J, Hou A F. Low temperature and excess moisture affect seed germination of soybean (Glycine max L.) under controlled environments. Can J Plant Sci, 2024, 104: 375–387.

[23] Zhang J P, Song Q J, Cregan P B, Nelson R L, Wang X Z, Wu J X, Jiang G L. Genome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm. BMC Genomics, 2015, 16: 217.

[24] Jin J, Wang J F, Li K K, Wang S W, Qin J, Zhang G H, Na X F, Wang X M, Bi Y R. Integrated physiological, transcriptomic, and metabolomic analyses revealed molecular mechanism for salt resistance in soybean roots. Int J Mol Sci, 2021, 22: 12848.

[25] Rasheed A, Raza A, Jie H D, Mahmood A, Ma Y S, Zhao L, Xing H C, Li L L, Hassan M U, Qari S H, et al. Molecular tools and their applications in developing salt-tolerant soybean (Glycine max L.) cultivars. Bioengineering, 2022, 9: 495.

[26] Verma O, Sharma S, Kumar V, Singh T, Kumar R, Auji R. Salinity stress effect on staple food crops and novel mitigation strategies. Biologia, 2024, 79: 2359–2374.

[27] 宝力格, 陆平, 史梦莎, 许月, 刘敏轩. 中国高粱地方种质芽期苗期耐盐性筛选及鉴定. 作物学报, 2020, 46: 734–753.
Bao L G, Lu P, Shi M S, Xu Y, Liu M X. Screening and identification of Chinese sorghum landraces for salt tolerance at germination and seedling stages. Acta Agron Sin, 2020, 46: 734–753 (in Chinese with English abstract).

[28] Zhao L, Li S M, He X M, Liu H Y, Cheng Y R, Wang Y, Kang H Y, Zeng J. Identification of salt-tolerant cultivars and plant traits in wheat during germination and seedling emergence stages. Plant Soil Environ, 2025, 71: 123–135.

[29] 李玉骁, 汪磊, 汪魏, 李军, 邬雪瑞, 朱梓榕, 王玲, 吴佳俊, 谭美莲. 向日葵种质资源的耐盐性评价. 植物遗传资源学报, 2024, 25: 1480–1492.
Li Y X, Wang L, Wang W, Li J, Wu X R, Zhu Z R, Wang L, Wu J J, Tan M L. Salt tolerance evaluation of sunflower germplasm resources. J Plant Genet Resour, 2024, 25: 1480–1492 (in Chinese with English abstract).

[30] 都润, 张思琦, 张海文, 陈涛. 逆境胁迫下向日葵的耐受机制. 生物技术进展, 2022, 12(2): 205–212.
Du R, Zhang S Q, Zhang H W, Chen T. The tolerance mechanism of sunflower under abiotic stress. Curr Biotechnol, 2022, 12(2): 205–212 (in Chinese with English abstract).

[31] Nikolić N, Ghirardelli A, Schiavon M, Masin R. Effects of the salinity-temperature interaction on seed germination and early seedling development: a comparative study of crop and weed species. BMC Plant Biol, 2023, 23: 446.

[1] 殷丛丛, 李睿琦, 岳霈尧, 李晨, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 基于闭合哑铃介导等温扩增可视化检测大豆花叶病毒SC15方法的建立及应用[J]. 作物学报, 2025, 51(5): 1248-1260.
[2] 金欣欣, 宋亚辉, 苏俏, 杨永庆, 李玉荣, 王瑾. 冀花系列高油酸花生抗旱性鉴定与综合评价[J]. 作物学报, 2025, 51(3): 797-811.
[3] 蒋优, 马雪融, 张博, 李陈建. 苏丹草种子萌发期耐盐性评价及耐盐种质筛选[J]. 作物学报, 2025, 51(3): 835-844.
[4] 钱玉平, 宿兵兵, 高吉星, 阮粉花, 李亚伟, 茅林春. 玉米大豆间作对喀斯特区土壤理化性质及微生物碳代谢特征的影响[J]. 作物学报, 2025, 51(1): 273-284.
[5] 丁树启, 程彤, 王弼琨, 于德彬, 饶德民, 孟凡钢, 赵胤凯, 王晓慧, 张伟. 密植对不同年代大豆品种群体光合生产和产量形成的影响[J]. 作物学报, 2025, 51(1): 161-173.
[6] 聂波涛, 刘德泉, 陈健, 崔正果, 侯云龙, 陈亮, 邱红梅, 王跃强. 北方春大豆品种农艺和品质性状分析与综合评价[J]. 作物学报, 2024, 50(9): 2248-2266.
[7] 闫锋, 董扬, 李清泉, 赵富阳, 侯晓敏, 刘洋, 李青超, 赵蕾, 范国权, 刘凯. 谷子育成品种萌芽期耐冷性综合评价[J]. 作物学报, 2024, 50(9): 2207-2218.
[8] 孙现军, 胡正, 姜雪敏, 王世佳, 陈向前, 张惠媛, 张辉, 姜奇彦. 大豆种质资源苗期耐盐性鉴定评价与筛选[J]. 作物学报, 2024, 50(9): 2179-2186.
[9] 刘欣玥, 郭潇阳, 王欣茹, 辛大伟, 关荣霞, 邱丽娟. 大豆萌发期耐盐性鉴定方法建立及耐盐大豆资源筛选[J]. 作物学报, 2024, 50(8): 2122-2130.
[10] 李晓菲, 高华伟, 广慧, 石宇欣, 谷勇哲, 齐照明, 邱丽娟. 大豆种质资源萌发期耐莠去津鉴定评价及优异种质筛选[J]. 作物学报, 2024, 50(7): 1699-1709.
[11] 张红梅, 张威, 王琼, 贾倩茹, 孟珊, 熊雅文, 刘晓庆, 陈新, 陈华涛. 大豆籽粒Ve含量的全基因组关联分析[J]. 作物学报, 2024, 50(5): 1223-1235.
[12] 苗龙, 舒阔, 李娟, 黄茹, 王业杏, Soltani Muhammad YOUSOF, 许竞好, 吴传磊, 李佳佳, 王晓波, 邱丽娟. 大豆根茎过渡区弯曲突变体Mrstz的鉴定与基因定位[J]. 作物学报, 2024, 50(5): 1091-1103.
[13] 李航, 刘丽, 黄乾, 刘文豪, 司爱君, 孔宪辉, 王旭文, 赵福相, 梅拥军, 余渝. 棉花种质资源萌发期耐盐性鉴定及筛选[J]. 作物学报, 2024, 50(5): 1147-1157.
[14] 陈家婷, 白欣, 谷雨杰, 张潇文, 郭慧娟, 常利芳, 陈芳, 张树伟, 张晓军, 李欣, 冯瑞云, 畅志坚, 乔麟轶. 小麦芽期和苗期耐盐鉴定方法的适用性评价[J]. 作物学报, 2024, 50(5): 1193-1206.
[15] 王亚琪, 徐海风, 李曙光, 傅蒙蒙, 余希文, 赵志鑫, 杨加银, 赵团结. 大豆类病变皱叶突变体NT301遗传分析和2对基因定位[J]. 作物学报, 2024, 50(4): 808-819.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2