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大豆开花时间和成熟期性状全基因组关联分析与候选基因预测

王琼1,邹丹霞1,陈兴运1,张威1,张红梅1,刘晓庆1,贾倩茹1,魏利斌2,崔晓艳1,陈新1,王学军2,* ,陈华涛1,*


  

  1. 1江苏省农业科学院经济作物研究所, 江苏南京 210014; 2江苏沿江地区农业科学研究所, 江苏南通 226541
  • 收稿日期:2024-09-29 修回日期:2025-03-26 接受日期:2025-03-26 网络出版日期:2025-04-07
  • 基金资助:
    本研究由江苏省自然科学基金项目(BK20220740), 江苏省重点研发计划项目(BE2022328)和江苏省种业振兴揭榜挂帅项目(JBGS[2021]057)资助。

Genome-wide association analysis and candidate genes prediction of flowering time and maturity date traits in soybean (Glycine max L.)

WANG Qiong1, ZOU Dan-Xia1, CHEN Xing-Yun1, ZHANG Wei1, ZHANG Hong-Mei1, LIU Xiao-Qing1, JIA Qian-Ru1,WEI Li-Bin2,CUI Xiao-Yan1,CEHN Xin1,WANG Xue-Jun2,*, CEHN Hua-Tao1,*   

  1. 1 Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China; 2 Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong 226541, Jiangsu, China
  • Received:2024-09-29 Revised:2025-03-26 Accepted:2025-03-26 Published online:2025-04-07
  • Supported by:
    This study was supported by the Natural Science Foundation of Jiangsu Province (BK20220740), the Key Research and Development Program of Jiangsu Province (BE2022328), and the Jiangsu Seed Industry Revitalization “Unveiling and Commanding” Project (JBGS[2021]057).

摘要:

大豆是典型的短日照作物,对光周期极为敏感,其栽培和产量均受到田间光周期条件的制约。本研究对264大豆种质资源的开花时间和成熟期性状进行了考察,分析了开花相关性状与蛋白质含量、含油量、百粒重和株高等农艺性状之间的关系。随后利用全基因组关联分析,鉴定出235个与开花时间和成熟期相关的位点,并预测了14个可能参与调控大豆开花时间和成熟期的候选基因,其中与开花时间相关的候选基因有10个,与成熟期相关的基因有5个,且1个候选基因同时与开花时间和成熟期相关。这些候选基因为进一步解析大豆开花相关性状的调控机制和大豆广适性高效遗传改良奠定了基础。

关键词: 大豆, 开花时间, 成熟期, 全基因组关联分析, SNP标记

Abstract:

Soybean is a typical short-day crop that is highly sensitive to photoperiod, with its cultivation and yield constrained by field photoperiodic conditions. In this study, we analyzed the flowering time and pod maturity date in 264 diverse soybean accessions. We examined the relationships between flowering-related traits and key agronomic traits, including protein content (PC), oil content (OC), 100-seed weight (HSW), and plant height (PH). A genome-wide association study (GWAS) identified 235 loci associated with flowering time and pod maturity date. Additionally, we predicted 14 candidate genes involved in the regulation of these traits, including 10 genes related to flowering time and 5 genes associated with pod maturity date. Notably, one gene exhibited pleiotropic effects on both traits. These findings provide valuable genomic insights into the regulatory pathways of flowering in soybean and offer a foundation for genetic improvement aimed at enhancing soybean adaptation across broader latitudinal regions.

Key words: soybean, flowering time, maturity date, GWAS, SNP markers

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