作物学报 ›› 2024, Vol. 50 ›› Issue (9): 2187-2206.doi: 10.3724/SP.J.1006.2024.31076
禹海龙1**(), 吴文雪1**(
), 裴星旭1, 刘晓宇1, 邓跟望2, 李西臣2, 甄士聪3, 望俊森3, 赵永涛3, 许海霞1, 程西永1, 詹克慧1,*(
)
YU Hai-Long1**(), WU Wen-Xue1**(
), PEI Xing-Xu1, LIU Xiao-Yu1, DENG Gen-Wang2, LI Xi-Chen2, ZHEN Shi-Cong3, WANG Jun-Sen3, ZHAO Yong-Tao3, XU Hai-Xia1, CHENG Xi-Yong1, ZHAN Ke-Hui1,*(
)
摘要:
小麦的茎秆性状与倒伏紧密相关, 发掘其显著关联的基因位点和候选基因, 为解析小麦茎秆性状的遗传机制和分子标记辅助育种提供依据。本研究以黄淮南部地区的192份小麦品种为材料, 利用小麦660K SNP芯片对7个环境成熟期和4个环境灌浆中期的14个茎秆性状进行了全基因组关联分析(GWAS), 同时利用RNA-seq分析了基部前二节间强度和直径的差异表达基因。结果表明, 所有性状的成熟期与灌浆中期的差异均达到极显著水平。与灌浆中期相比, 成熟期的重心高度增幅较大, 基部第二和第三节间长度增加, 其他性状均降低。GWAS共检测出163个与茎秆性状相关的稳定SNP标记(MTA), 其中45个具有基因或蛋白注释, 与转录组联合分析, 共筛选到3个与小麦茎秆性状相关的候选基因。推测候选基因TraesCS5A02G522100通过合成异戊二酸类化合物调节小麦的光合作用以及通过调节固醇的生成提高细胞膜的稳定性和渗透性, 进而促进茎秆的发育, 改善茎秆性状。候选基因TraesCS1D02G390600直接参与小麦茎秆细胞分裂和器官的形成, 使茎秆变粗, 充实度增加。候选基因TraesCS7A02G481800在小麦中通过参与细胞间的信号传递过程, 调控茎秆的发育、应激响应等过程。这些候选基因通过qRT-PCR验证, 其基因的表达趋势与转录组测序所得结果基本一致。
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