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作物学报 ›› 2025, Vol. 51 ›› Issue (2): 548-556.doi: 10.3724/SP.J.1006.2025.44090

• 研究简报 • 上一篇    

花生主茎节间和侧枝节间长度的关联作图及候选基因分析

赵斐斐1,2，李少雄2，刘浩2，李海芬2，王润风2，黄璐2，余倩霞2，洪彦彬2，陈小平2，鲁清2,*，曹玉曼1,*   

  1. 1西北农林科技大学草业与草原学院, 陕西杨凌712100; 2广东省农业科学院作物研究所 / 广东省农作物遗传改良重点实验室 / 国家油料作物改良中心南方花生分中心, 广东广州510640
  • 收稿日期:2024-06-03 修回日期:2024-09-18 接受日期:2024-09-18 出版日期:2025-02-12 网络出版日期:2024-10-11
  • 通讯作者: 曹玉曼, E-mail: yumancao@nwafu.edu.cn; 鲁清, E-mail:luqing2016@126.com
  • 基金资助:
    本研究由国家重点研发计划项目(2023YFD1202800), 财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13), “十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(2022SDZG05), 广东省重点领域研发计划项目-现代种业(2022B0202060004)和广东特支计划项目(2021TX06N789)资助。

Association mapping of internode and lateral branch internode length of peanut main stem and analysis of candidate genes

ZHAO Fei-Fei1,2,LI Shao-Xiong2,LIU Hao2,LI Hai-Fen2,WANG Run-Feng2,HUANG Lu2,YU Qian-Xia2,HONG Yan-Bin2,CHEN Xiao-Ping2,LU Qing2,*,CAO Yu-Man1,*   

  1. 1College of Grassland Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China; 2 Crops Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Crop Genetic Improvement / South China Peanut Sub-Centre of National Centre of Oilseed Crops Improvement, Guangzhou 510640, Guangdong, China
  • Received:2024-06-03 Revised:2024-09-18 Accepted:2024-09-18 Published:2025-02-12 Published online:2024-10-11
  • Supported by:
    This study was supported by the National Key Research and Development Program of China (2023YFD1202800), the China Agriculture Research System of MOF and MARA (CARS-13), the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan in Guangdong Province (2022SDZG05), the Guangdong Provincial Key Research and Development Program-Modern Seed Industry (2022B0202060004), and the Special Support Program of Guangdong Province (2021TX06N789).

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摘要：

花生主茎节间和侧枝节间长度是影响单株产量的重要农艺性状。本研究以390份花生自然群体为研究材料，在花生成熟期分别测量主茎与侧枝的第一、第二、第三节间长度。选用GAPIT3.0软件中的混合线性模型(PCA+K模型)进行全基因组关联分析。结果显示，主茎、侧枝节间长度基本符合正态分布，主茎节间与侧枝节间存在显著正相关。检测到63个主茎节间、侧枝节间长度相关位点。根据关联情况，找到3个显著性关联位点和位点簇。在A04_57397319挖掘到1个与前人共定位的显著性关联位点，并在该处预测到5个候选基因。本研究结果有助于解析花生主茎节间和侧枝节间的遗传基础和调控机制，为指导株型010改良奠定基础。

关键词: 花生, 主茎节间长度, 侧枝节间长度, 全基因组关联分析

Abstract:

The internode length of the main stem and lateral branches is a key agronomic trait influencing the yield per plant in peanuts. In this study, 390 natural peanut populations were used to measure the length of the first, second, and third internodes of both the main stem and lateral branches at maturity. A genome-wide association analysis was conducted using the mixed linear model (PCA+K model) in GAPIT3.0 software. The results showed that the internode lengths of the main stem and lateral branches followed a normal distribution and were significantly positively correlated. A total of 63 loci associated with the internode length of the main stem and lateral branches were identified. Three significant association sites and site clusters were discovered, including a notable association at A04_57397319 that co-located with findings from previous research. Five candidate genes were predicted within this region. These findings provide valuable insights into the genetic basis and regulatory mechanisms of internode length in both the main stem and lateral branches of peanuts, laying a foundation for plant architecture improvement.

Key words: peanut, main stem internode length, lateral branch internode length, GWAS

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